Contents - Volume 55 (2010)

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Important notice for authors and readership

Beginning with the first issue of volume 56 (year 2011) Folia Microbiologica will be published exclusively in an electronic form. At the same time, both the procedure for submitting the manuscripts to FM and the Instructions for Authors will be changed. The Editorial Office will from now on use the electronic Editorial Manager of Springer. For any further information please see internet pages of Folia Microbiologica (http://www.biomed.cas.cz/mbu/folia/) or the pages of the journal at SpringerLink (http://springerlink.metapress.com/content/120945).

 

If you are interested in obtaining full texts of Folia Microbiologica papers (vol. 55, 2010) in PDF format, please, go to the www page http://springerlink.metapress.com/content/120945

Folia Microbiologica 1/10  [FOMIAZ 55 (1) 1-104 (2010); ISSN 0015-5632]
3 A. Zieba, R.D. Wojtyczka, M. Kepa, D. Idzik
Azinyl Sulfides – CXVIII. Antimicrobial Activity of Novel 1-Methyl-3-thio-4-aminoquinolinium salts
10 P.W. Yang, M.G. Li, J.Y. Zhao, M.Z. Zhu, H. Shang, J.R. Li, X.L. Cui, R. Huang, M.L. Wen
Oligomycins A and C, Major Secondary Metabolites Isolated from the Newly Isolated Strain Streptomyces diastaticus
17 S. Obruca, I. Marova, Z. Svoboda, R. Mikulikova
Use of Controlled Exogenous Stress for Improvement of Poly(3-hydroxybutyrate) Production in Cupriavidus necator
23 H. Ouled-Haddar, T.I. Zaghloul, H.M. Saeed
Expression of Alkaline Proteinase Gene in Two Recombinant Bacillus cereus Feather-Degrading Strains
29 K. Fujii, T. Sugimura, K. Nakatake
Ascomycetes with Cellulolytic, Amylolytic, Pectinolytic, and Mannanolytic Activities Inhabiting Dead Beech (Fagus crenata) Trees
35 M. Yaman, O. Erturk, I. Aslan
Isolation of Some Pathogenic Bacteria from the Great Spruce Bark Beetle, Dendroctonus micans and Its Specific Predator, Rhizophagus grandis
39 M. Bonadei, C. Calvio, D. Carbonera, A. Galizzi, E. Quattrini, A. Balestrazzi
Spore-Forming Bacteria in Soil Cultivated with GM White Poplars Isolation and Characterization
47 M.H. Abd-Alla, S.R. Bashandy, S. Schnell
Occurrence of Xanthomonas axonopodis pv. phaseoli, the Causal Agent of Common Bacterial Blight Disease, on Seeds of Common Bean (Phaseolus vulgaris L.) in Upper Egypt
53 K. Chen, L. Song, B. Rao, T. Zhu, Y.T. Zhang
Nitric oxide Plays a Role as Second Messenger in the Ultraviolet-B Irradiated Green Alga Chlorella pyrenoidosa
61 M.M. El-Sheekh, Y.A.-G. Mahmoud, A.M. Abo-Shady, W. Hamza
Efficacy of Rhodotorula glutinis and Spirulina platensis Carotenoids in Immunopotentiation of Mice Infected with Candida albicans SC5314 and Pseudomonas aeruginosa 35
69 A.K. Goel, M. Jain, P. Kumar, D.V. Kamboj, L. Singh
Virulence Profile and Clonal Relationship among the Vibrio cholerae Isolates from Ground and Surface Water in a Cholera Endemic Area during Rainy Season
75 A. Hostacka, I. Ciznar, M. Stefkovicova
Temperature and pH Affect the Production of Bacterial Biofilm
79 V. Kmet, M. Kmetova
High Level of Quinolone Resistance in Escherichia coli from Healthy Chicken Broilers
83 P. Hejnar, M. Kolar, P. Sauer
Antibiotic Resistance of Stenotrophomonas maltophilia Strains Isolated from Captive Snakes
88 J. Schramlova, S. Arientova, D. Hulinska
The Role of Electron Microscopy in the Rapid Diagnosis of Viral Infections – review
102 P. Olejnikova, Z. Ondrusova, S. Krystofova, D. Hudecova
Benomyl-Resistant Mutant Strain of Trichoderma sp. with Increased Mycoparasitic Activity
 

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Folia Microbiologica 2/10  [FOMIAZ 55 (2) 105-200 (2010); ISSN 0015-5632]
107 S. Lew, M. Lew, T. Mieszczyński, J. Szarek
Selected Fluorescent Techniques for Identification of the Physiological State of Individual Water and Soil Bacterial Cells – review
119 R. Novakova, Z. Odnogova, P. Kutas, L. Feckova, J. Kormanec
Identification and Characterization of an Indigoidine-like Gene for a Blue Pigment Biosynthesis in Streptomyces aureofaciens CCM 3239
126 R. Smidak, M. Jopcik, M. Kralovicova, J. Gajdosikova, J. Kormanec, J. Timko, J. Turna
Core Promoters of the Penicillin Biosynthesis Genes and Quantitative RT-PCR Analysis of These Genes in High and Low Production Strain of Penicillium chrysogenum
133 N. Rashid, N. Ahmed, M. Saleem Haider, I. Haque
Effective Solubilization and Single-Step Purification of Bacillus licheniformis α-Amylase from Insoluble Aggregates
137 M.Y. Shukor, M.F. Rahman, Z. Suhaili, N.A. Shamaan, M.A. Syed
Hexavalent Molybdenum Reduction to Mo-blue by Acinetobacter calcoaceticus
145 R. Jankowiak, M. Kolarik
Diversity and Pathogenicity of Ophiostomatoid Fungi Associated with Tetropium Species Colonizing Picea abies in Poland
155 L. Alves Scheid, D.A. Nunes Mario, I.H. Soares, E. Silva Loreto, J.M. Santurio, S. Hartz Alves
DRBC Agar: a New Tool for Candida dubliniensis Identification
159 Zhu-Mei He, Jing Chen, Hong-Zhou Li, Di-Qing Luo, Wei Yang
Mutants of Candida albicans Hypersensitive to Calcofluor White Display Susceptibility to Antifungal Drugs
167 A. Krutkiewicz, D. Klimuszko
Genotyping and PCR Detection of Potential Virulence Genes in Campylobacter jejuni and Campylobacter coli Isolates from Different Sources in Poland
176 O.A. Aiyegoro, A.J. Afolayan, A.I. Okoh
Interactions of Antibiotics and Extracts of Helichrysum pedunculatum against Bacteria Implicated in Wound Infections
181 Z. Zidek, E. Kmonickova, P. Kostecka, H. Tlaskalova-Hogenova
Decisive Role of Lipopolysaccharide in Activating Nitric Oxide and Cytokine Production by the Probiotic Escherichia coli strain Nissle 1917
191 M. Holicka, J. Novosad, M. Kudlova, M. Loudova, C. Andrys, J. Krejsek
J774 Macrophage-like Cell Line Cytokine and Chemokine Patterns are Modulated by Francisella tularensis LVS Strain Infection
 

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Folia Microbiologica 3/10  [FOMIAZ 55 (3) 201-304 (2010); ISSN 0015-5632]
203 M. Holub, S. Bezouskova, D. Petrackova, L. Kalachova, O. Kofronova, O. Benada, J. Weiser
Comparative Study of the Life Cycle Dependent Post-Translation Modifications of Protein Synthesis Elongation Factor Tu Present in the Membrane Proteome of Streptomycetes and Mycobacteria
211 N.O. Musa, K. Eltom, F. Gessler, H. Bohnel, A. Babiker, S.M. El Sanousi
The Catalase Gene Differentiates between Some Strains of Staphylococcus aureus ssp. anaerobius
215 Z. Molatova, E. Skrivanova, B. Macias, N.R. McEwan, P. Brezina, M. Marounek
Susceptibility of Campylobacter jejuni to Organic Acids and Monoacylglycerols
221 S. Kumari, K. Harjai, S. Chhibber
Isolation and Characterization of Klebsiella pneumoniae Specific Bacteriophages from Sewage Samples
228 M.A.S.C. Chellegatti, P.D. Yuvamoto, S. Said
Role of Phospholipase C and Protein Kinase C in Aspergillus nidulans during Growth on Pectin or Glucose: Effects on Germination and Duplication Cycle
233 Kanika, Tripti Dogra, Lata
Biochemical and Molecular Characterization of a Rhizobitoxine-Producing Bradyrhizobium from Pigeon Pea Plants
239 B. Kokoskova, I. Mraz, J. Fousek
Comparison of Specificity and Sensitivity of Immunochemical and Molecular Techniques for Determination of Clavibacter michiganensis ssp. michiganensis
245 Qingguo Meng, Wei Gu, Keran Bi, Huyan Ji, Wen Wang
Spiralin-like Protein Slp31 from Spiroplasma eriocheiris as a Potential Antigen for Immunodiagnostics of Tremor Disease in Chinese Mitten Crab Eriocheir sinensis
251 F. Quaglino, P. Casati, P.A. Bianco
Distinct rpsC Single Nucleotide Polymorphism Lineages of Flavescence Dorée Subgroup 16SrV-D Phytoplasma Co-Infect Vitis vinifera L.
258 L. Gao, W.Q. Chen, T.G. Liu
Development of a SCAR Marker by Inter-Simple Sequence Repeat for Diagnosis of Dwarf Bunt of Wheat and Detection of Tilletia controversa Kühn
265 T. Rezanka, M. Petrankova, V. Cepak, P. Pribyl, K. Sigler, T. Cajthaml
Trachydiscus minutus, a New Biotechnological Source of Eicosapentaenoic acid
270 Z.H. Sun, W.J. Liu, J.H. Zhang, J. Yu, W. Gao, M. Jiri, B. Menghe, T.S. Sun, H.P. Zhang
Identification and Characterization of the Dominant Lactic acid Bacteria Isolated from Traditional Fermented Milk in Mongolia
277 B. Cukrowska, I. Rosiak, E. Klewicka, I. Motyl, M. Schwarzer, Z. Libudzisz, H. Kozakova
Impact of Heat-Inactivated Lactobacillus casei and Lactobacillus paracasei Strains on Cytokine Responses in Whole Blood Cell Cultures of Children with Atopic Dermatitis
281 M. Grmanova, E. Vlkova, V. Rada, I. Homutov
Survival of Bifidobacteria in Adult Intestinal Tract
286 O. Nyc, J. Matejkova
Stenotrophomonas maltophilia: Significant Contemporary Hospital Pathogen – review
295 I. Trebichavsky, I. Splichal, A. Splichalova
Innate Immune Response in the Gut against Salmonellareview
301 T.-H. Kim, M.-K. Lee
Evaluation of the V404I and V509M Amino Acid Substitutions of ERG11 Gene in Candida albicans Isolates by Pyrosequencing
 

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Folia Microbiologica 4/10  [FOMIAZ 55 (4) 305-408 (2010); ISSN 0015-5632]
307 6th International Symposium of Anaerobic Microbiology (ISAM 2009)
309 R.C. Anderson, M.D. Flythe, N.A. Krueger, T.R. Callaway, T.S. Edrington, R.B. Harvey, D.J. Nisbet
Decreased Competiveness of the Foodborne Pathogen Campylobacter jejuni during Co-Culture with the Hyper-Ammonia Producing Anaerobe Clostridium aminophilum
312 G. Belzecki, R. Miltko, E. Kwiatkowska, T. Michalowski
Mureinolytic Ability of the Rumen Ciliate Diploplastron affine
315 L. Fecskeova, M. Piknova, P. Javorsky, P. Pristas
Lactate Dehydrogenase Gene Variability among Predominant Lactate Utilizing Ruminal Bacteria
319 K. Fliegerova, J. Mrazek, K. Hoffmann, J. Zabranska, K. Voigt
Diversity of Anaerobic Fungi within Cow Manure Determined by ITS1 Analysis
326 M. Grmanova, V. Rada, K. Sirotek, E. Vlkova
Naturally Occurring Prebiotic Oligosaccharides in Poultry Feed Mixtures
329 A. Kasperowicz, K. Stan-Glasek, W. Guczynska, M. Piknova, P. Pristas, K. Nigutova, P. Javorsky, T. Michalowski
Fructanolytic and Saccharolytic Enzymes of the Rumen Bacterium Pseudobutyrivibrio ruminis Strain 3 – preliminary study
332 V. Kmet, E. Piatnicova
Antibiotic Resistance in Commensal Intestinal Microflora
336 J. Kopecny, J. Mrazek, J. Killer
The Presence of Bifidobacteria in Social Insects, Fish and Reptiles
340 M. Linhova, P. Patakova, J. Lipovsky, P. Fribert, L. Paulova, M. Rychtera, K. Melzoch
Development of Flow Cytometry Technique for Detection of Thinning of Peptidoglycan Layer as a Result of Solvent Production by Clostridium pasteurianum
345 F. Lukas, J. Simunek, J. Mrazek, J. Kopecny
PCR-DGGE Analysis of Bacterial Population Attached to the Bovine Rumen Wall
349 R. Miltko, G. Belzecki, E. Kwiatkowska, T. Michalowski
The Ability of the Rumen Protozoan Eudiplodinium maggii to Utilize Chitin
352 J. Mrazek, I. Koppova, J. Kopecny, J. Simunek, K. Fliegerova
PCR-DGGE-Based Study of Fecal Microbial Stability during the Long-Term Chitosan Supplementation of Humans
359 Z. Novakova, J. Blasko, I. Hapala, P. Smigan
Effects of 3-Hydroxy-3 methylglutaryl-coenzyme A Reductase Inhibitor Pravastatin on Membrane Lipids and Membrane Associated Functions of Methanothermobacter thermautotrophicus
363 Z. Novotna, J. Prochazka, J. Simunek, K. Fliegerova
Xylanases of Anaerobic Fungus Anaeromyces mucronatus
368 M. Popova, C. Martin, D.P. Morgavi
Improved Protocol for High-Quality Co-Extraction of DNA and RNA from Rumen Digesta
373 Z. Sefcikova, V. Kmet, D. Bujnakova, L. Racek, S. Mozes
Development of Gut Microflora in Obese and Lean Rats
376 L. Sikorova, M. Piknova, P. Javorsky, W. Guczynska, A. Kasperowicz, T. Michalowski, P. Pristas
Variability of Treponemes in the Rumen of Ruminants
379 J. Simunek, I. Koppova, L. Filip, G. Tishchenko, G. Belzecki
The Antimicrobial Action of Low-Molar-Mass Chitosan, Chitosan Derivatives and Chitooligosaccharides on Bifidobacteria
383 K. Stan-Glasek, A. Kasperowicz, W. Guczynska, M. Piknova, P. Pristas, K. Nigutova, P. Javorsky, T. Michalowski
Phosphorolytic Cleavage of Sucrose by Sucrose-Grown Ruminal Bacterium Pseudobutyrivibrio ruminis Strain k3
386 G. Tishchenko, I. Koppova, J. Simunek, J. Dohnalek
Extracellular Complex of Chitinolytic Enzymes of Clostridium paraputrificum Strain J4 Separated by Membrane Ultrafiltration
390 E. Vlkova, M. Grmanova, J. Killer, J. Mrazek, J. Kopecny, V. Bunesova, V. Rada
Survival of Bifidobacteria Administered to Calves

393 Z. Lorincz, E. Preininger, A. Kosa, T. Ponyi, P. Nyitrai, L. Sarkadi, G.M. Kovacs, B. Boddi, I. Gyurjan
Artificial Tripartite Symbiosis Involving a Green Alga (Chlamydomonas), a Bacterium (Azotobacter) and a Fungus (Alternaria): Morphological and Physiological Characterization
401 J.J. Malago, E. Nemeth, J.F.J.G. Koninkx, P.C.J. Tooten, S. Fajdiga, J.E. van Dijk
Microbial Products from Probiotic Bacteria Inhibit Salmonella enteritidis 857-Induced IL 8 Synthesis in Caco-2 Cells
 

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Folia Microbiologica 5/10  [FOMIAZ 55 (5) 409-532 (2010); ISSN 0015-5632]
411 J. Trogl, J. Halova, G. Kuncova, P. Parik
Automatic Formation of Hypotheses on the Relationships between Structure of Naphthalene Analogs and Bioluminescence Response of Bioreporter Pseudomonas fluorescens HK44
418 E. Petrlikova, K. Waisser, P. Jilek, I. Dufkova
Antibacterial Activity of N-Benzylsalicylthioamides
422 O.A. Oluduro, B.I. Aderiye, J.D. Connolly, E.T. Akintayo, O. Famurewa
Characterization and Antimicrobial Activity of 4-(β-d-Glucopyranosyl-1→4-α-l-rhamnopyranosyloxy)-benzyl thiocarboxamide; a Novel Bioactive Compound from Moringa oleifera Seed Extract
427 D. Petrackova, L. Semberova, P. Halada, P. Svoboda, J. Svobodova
Stress Proteins in the Cytoplasmic Membrane Fraction of Bacillus subtilis
435 Y. Krauke, H. Sychrova
Chimeras between C. glabrata Cnh1 and S. cerevisiae Nha1 Na+/H+-Antiporters Are Functional Proteins Increasing the Salt Tolerance of Yeast Cells
442 J. Raj, S. Prasad, N.N. Sharma, T.C. Bhalla
Bioconversion of Acrylonitrile to Acrylamide using Polyacrylamide Entrapped Cells of Rhodococcus rhodochrous PA-34
447 H. Lee, Y.-S. Choi, M.-J. Kim, N.-Y. Huh, G.-H. Kim, Y. W. Lim, S.-M. Kang, S.-T. Cho, J.-J. Kim
Degrading Ability of Oligocyclic Aromates by Phanerochaete sordida Selected via Screening of White Rot Fungi
454 M. Gryndler, L. Soukupova, H. Gryndlerova, P. Baldrian, H. Hrselova
Local Distribution of Ectomycorrhizae-Associated Basidiomycetes in Forest Soil Correlates with the Degree of Soil Organic Matter Humification and Available Electrolytes
461 A.K. Borsodi, R.I. Kiss, G. Cech, B. Vajna, E.M. Toth, K. Marialigeti
Diversity and Activity of Cultivable Aerobic Planktonic Bacteria of a Saline Lake Located in Sovata, Romania
467 K. Hrckova, M. Simek, P. Hrouzek, A. Lukesova
Biological Dinitrogen Fixation by Selected Soil Cyanobacteria as Affected by Strain Origin, Morphotype, and Light Conditions
474 R. Prasanna, K. Madhan, R.N. Singh, A.K. Chauhan, L. Nain
Developing Biochemical and Molecular Markers for Cyanobacterial Inoculants
481 L.H. Markiewicz, E. Biedrzycka, E. Wasilewska, M. Bielecka
Rapid Molecular Identification and Characteristics of Lactobacillus Strains
489 B.A. Knapp, J. Seeber, A. Rief, E. Meyer, H. Insam
Bacterial Community Composition of the Gut Microbiota of Cylindroiulus fulviceps (Diplopoda) as Revealed by Molecular Fingerprinting and Cloning
497 B. Sadowska, E. Walencka, M. Wieckowska-Szakiel, B. Rozalska
Bacteria Competing with the Adhesion and Biofilm Formation by Staphylococcus aureus
502 V. Seputiene, A. Vilkoicaite, J. Armalyte, A. Pavilonis, E. Suziedeliene
Detection of Methicillin-Resistant Staphylococcus aureus using Double Duplex Real-Time PCR and Dye Syto 9
508 M. Palusinska-Szysz, M. Janczarek
Innate Immunity to Legionella and Toll-like Receptors – review
515 J. Hrdy, P. Zanvit, O. Novotna, I. Kocourkova, J. zizka, L. Prokesova
Cytokine Expression in Cord Blood Cells of Children of Healthy and Allergic Mothers
520 S. Kumar, S. Kapil, V. Gautam, S.K. Verma, P. Ray
Peptides Representing the Specific Variable Regions but not the Full Porin Proteins Can Be Useful for Antibody Based Diagnosis of Typhoid Fever
528 K. Hulikova, V. Grobarova, R. Krivohlava, A. Fiserova
Antitumor Activity of N-Acetyl-d-glucosamine-Substituted Glycoconjugates and Combined Therapy with Keyhole Limpet Hemocyanin in B16F10 Mouse Melanoma Model
 

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Folia Microbiologica 6/10  [FOMIAZ 55 (6) 533-672 (2010); ISSN 0015-5632]
535 M. Duran, A. Faljoni-Alario, N. Duran
Chromobacterium violaceum and Its Important Metabolites – review
548 O. Melter, B. Radojevic
Small Colony Variants of Staphylococcus aureus – review
559 E. Lencova, Z. Broukal, J. Spizek
Point-of-Care Salivary Microbial Tests for Detection of Cariogenic Species – Clinical Relevance Thereof – review
569 J. Trogl, G. Kuncova, P. Kuran
Bioluminescence of Pseudomonas fluorescens HK44 in the Course of Encapsulation into Silica Gel. Effect of Methanol
576 A. Poli, G. Anzelmo, G. Tommonaro, K. Pavlova, A. Casaburi, B. Nicolaus
Production and Chemical Characterization of an Exopolysaccharide Synthesized by Psychrophilic Yeast Strain Sporobolomyces salmonicolor AL1 Isolated from Livingston Island, Antarctica
582 K.Y. Wu, S.H. Huang, S. Ding, Y.K. Zhang, G.G. Chen, Z.Q. Liang
Expression, Purification and Characterization of Recombinant α-Glucosidase in Pichia pastoris
588 S.M.A. Abdel Malek, Y.R. Badran
Pseudomonas aeruginosa PAO1 Adapted to 2 Phenoxyethanol Shows Cross-Resistance to Dissimilar Biocides and Increased Susceptibility to Antibiotics
593 E. Casalone, E. Bonelli, M. Polsinelli
Effects of Mancozeb and Other Dithiocarbamate Fungicides on Saccharomyces cerevisiae: the Role of Mitochondrial petite Mutants in Dithiocarbamate Tolerance
598 V. Chebenova, G. Berta, T. Kuchta, B. Brezna, D. Pangallo
Randomly-Amplified Microsatellite Polymorphism for Preliminary Typing of Lactic Acid Bacteria from Bryndza Cheese
603 W. Zhang, Z. Sun, T. Sun, H. Zhang
PCR Screening and Sequence Analysis of iol Clusters in Lactobacillus casei Strains Isolated from Koumiss
607 J. Cao, J. Xu, Q. Zheng, P. Yan
Rapid Detection of Vibrio metschnikovii in Aquatic Products by Real-Time PCR
614 D. Jain, S.K. Mishra, A. Shrivastav, M. Rathod, B.D. Shethia, S. Mishra, B. Jha
Poly-Extremotolerant Bacterium Isolated from Reverse Osmosis Reject: an Implication toward Waste Water Management
621 G. Roeselers, M.C.M. van Loosdrecht
Microbial Phytase-Induced Calcium-phosphate Precipitation – a Potential Soil Stabilization Method
625 Z. Zizka, T. Vetrovský, J. Gabriel
Enhancement of Autofluorescence of the Brown-Rot Fungus Piptoporus betulinus by Metal Ions
629 S.C. Dubey, A. Tripathi, S.R. Singh
ITS-RFLP Fingerprinting and Molecular Marker for Detection of Fusarium oxysporum f.sp. ciceris
635 M. Hricinová, E. Holoda, D. Mudronova, S. Ondrasovicova
Multiplex PCR Assay for Detection of Actinobacillus pleuropneumoniae, Pasteurella multocida and Haemophilus parasuis in Lungs of Pigs from a Slaughterhouse
641 A. Salerno, I. Ciznar, K. Krovacek, M. Conte, S. Dumontet, C. Gonzalez-Rey, V. Pasquale
Phenotypic Characterization and Putative Virulence Factors of Human, Animal and Environmental Isolates of Plesiomonas shigelloides
649 M. Hybenova, P. Hrda, B. Potuznikova, E. Pavlik, V. Stejskal, J. Dosedel, I. Sterzl
Lymphocyte Proliferative Response to Helicobacter pylori Antigens in H. pylori-Infected Patients
657 M. Kapral, L. Weglarz, B. Parfiniewicz, J. Lodowska, M. Jaworska-Kik
Quantitative Evaluation of Transcriptional Activation of NF-κB p65 and p50 Subunits and IκBα Encoding Genes in Colon Cancer Cells by Desulfovibrio desulfuricans Endotoxin
662 Z. Zizka
Newly Designed, Simple Relief Phase Contrast for Microscopy of Microorganisms
666 M. Hijazin, H. Ulbegi-Mohyla, J. Alber, C. Lammler, A.A. Hassan, E. Prenger-Berninghoff, R. Weiss, M. Zschock
Identification of Arcanobacterium haemolyticum Isolated from Postcastrational Complications of a Horse
669 R. Kolinska, M. Drevinek, E. Aldova, H. Zemlickova
Identification of Plesiomonas spp.: Serological and MALDI-TOF MS Methods

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