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Improving determinations tools in Aspergillus
Improving determinations tools in Aspergillus
Aspergillus (Ascomycota: Eurotiales) is genus of filamentous fungi comprising biotechnologically and toxicologically important fungi. More than 60 species have been isolated from clinical material and several species are serious human pathogens. One of the most widely used determinations genetic markers is ß-tubulin gene, which is known in two isomorphs (benA and tubC). Whereas benA is widely used, tubC distribution within the genus and its structure is poorly known. There is no reliable tool to distinguish both isomorphs, what results in illegitimate using of tubC instead of benA sequences. We are focused in determination of benA and tubC structure in all Aspergillus linages. The aim is to provide tool for distinguishing of both isophorms and find specific primers which should be used for routing taxonomic determinations. The revision of Aspergillus isolates held in
Czech culture collection is conducted in order to obtain reliable determined material for comparisons. Our results substantially increased knowledge about identity of isolates from clinical material including finding of very rare or already undescribed species.
A diagram showing variability and divergence of benA and tubC structure in Aspergillus. The divergence between both isomorphs could be relatively low and their structure in the same species similar, what results in difficulties in their distinguishing.
