Institute of Microbiology of Czech Acad Sci
Vídeňská 1083
142 20 Prague 4
Czech Republic
PHONE: +420 241 062 503
FAX: +420 241 062 501

oCellaris - usefull SW to analyse pH and stress granules in yeast


Jiří Hašek, PhD. (head) hasek [at] +420 241 062 503
Pavla Binarová, PhD., binarova [at] +420 241 062 130
Libuše Váchová, PhD., vachova [at] +420 325 873 929
Jana Vojtová, PhD. jana.vojtova [at] +420 241 062 504
Otakar Hlaváček, PhD. hlavacek [at] +420 325 873 601
Stanislava Rešetárová, PhD. resetarova [at] +420 241 062 504
Lucie Trögelová (Kohoutová), PhD. troglova [at] (maternity leave) +420 241 062 130
Jana Chumová, PhD. chumova [at] +420 241 062 130
Hana Kourová, PhD. kourova [at] +420 241 062 130
Pavla Novotná (PhD student) pavla.novotna [at] +420 325 873 601
Michaela Humpálová, MSc. (asistent) martina.pavlickova [at] +420 325 873 601
Michaela Zázvorková, MSc. (asistent) michaela.zazvorkova [at] +420 325 873 601
Lenka Nováková, MSc. (asistent) lenkan [at] +420 241 062 590
Danuša Hašková, MSc. (asistent) danusa.haskova [at] +420 241 062 590
Alexandra Pokorná(technician) pokorna [at] +420 325 873 601


i) Stress response
ii) Cytoskeleton
iii) Cell communities.


The laboratory is focused on the analysis of the spatial control and the dynamics of gene expression and proteostasis in eukaryotic cells, specifically in response to stress and aging.

The scientific topics of the laboratory are the following:

1.Protein quality control, autophagy, cell vitality, cell aging, and apoptosis as affected by genetic factors, environmental and intracellular stressors like heat, toxins, radiation, chemicals, reactive oxygen, and nitrogen species. Studies of the dynamics of the stress response, onset and course of apoptosis. Studies of the effect of different stresses on membrane structure. Studies are performed in liquid cultures of wild-type or mutant cells, and also in the context of multicellular cell communities (giant colonies).

2.Regulatory functions of cytoskeleton under physiological and stress conditions and in aging. Study of gamma-tubulin functions in connecting nuclei organization with cytoskeleton dynamics in dividing and differentiated cells. Study of actin dynamics in cell division and differentiation. To analyze the expression of specific proteins of interest and changes in their behavior and distribution we construct various wild-type or mutant versions of protein fusions with GFP, mRFP, mCherry, yTagRFP-T and ymTagBFP from their chromosomal sites or from plasmids in Saccharomyces cerevisiae and Arabidopsis thaliana. The yeast cells and plant cell lines are used in live-cell imaging, biochemical and proteomics studies.  

2017 - Prague - 28th International Conference on Yeast Genetics and Molecular Biology


The projects are financially supported by the following institutions:
Czech Science Foundation: 16-05497S (JH), P501 15-11657S (PB)


NICHD NIH, Bethesda, USA (Dr. A. Hinnebusch)
University of Salzburg, Austria (Prof. M. Breitenbach, Dr. M. Rinnerthaler)
St John's University, New York, USA (Dr. Ales Vancura)
University of New Hampshire, Durham, USA (Dr. Clyde L.Denis)
Swedish University of Agricultural Sciences, Uppsala, Sweden (G. Daniel)
University of London, UK (L. Bögre)
Semmelweis University, Budapest, Hungary (T. Mészáros)


Vojtova J., Hasek J. (2020)Mmi1, the Yeast Ortholog of Mammalian Translationally Controlled Tumor Protein (TCTP), Negatively Affects Rapamycin-Induced Autophagy in Post-Diauxic Growth Phase. Cells (2020) 9(1):138. doi: 10.3390/cells9010138. PMID: 31936125; PMCID: PMC7017036.

Maršíková J., Pavlíčková M. , Wilkinson D.,Váchová L., Hlaváček O. , Hatáková L., Palková Z. (2020)The Whi2p-Psr1p/Psr2p complex regulates interference competition and expansion of cells with competitive advantage in yeast colonies.Proc Natl Acad Sci USA (2020)117:15123-15131

Kállai B.M., Kourová H., Chumová J., Papdi C., Trögelová L., Kofroňová O., Hozák P., Filimonenko V., Mészáros T., Magyar Z., Bögre L., Binarová P. (2020)γ-Tubulin interacts with E2FA, E2FB and E2FC transcription factors, regulates proliferation and endocycle in Arabidopsis. Journal of Experimental Botany (2020) 71:1265–1277

Li J., Rinnerthaler M., Hartl J.,Weber M., Karl T., Breitenbach-Koller H., Mülleder M., Vowinckel J., Marx H., Sauer M., Mattanovich D., Ata O., De S., Greslehner G.P., Geltinger F., Burhans B., Grant Ch., Doronina V., Ralser M., Streubel M.K., Grabner Ch., Jarolim S., Moßhammer C., Gourlay C.W., Hasek J., Cullen P.J., Litti J., Ralser M. and Breitenbach M. (2020)Slow Growth and Increased Spontaneous Mutation Frequency in Respiratory Deficient afo1 Yeast Suppressed by a Dominant Mutation in ATP3 G3: Genes, Genomes, Genetics (2020)10:4637-4648

Van Nguyen P., Plocek V., Váchová L. , Palková Z. (2020)Glucose, Cyc8p and Tup1p regulate biofilm formation and dispersal in wild Saccharomyces cerevisiae. NPJ Biofilms Microbiomes (2020)6(1):7

Plocek V., Váchová L., Šťovíček V., Palková Z. (2020)Cell Distribution within Yeast Colonies and Colony Biofilms: How Structure Develops. Int J Mol Sci. (2020)21:3873

Váchová L, Palková Z. (2019)Diverse roles of Tup1p and Cyc8p transcription regulators in the development of distinct types of yeast populations. Curr Genet. (2019)65:147–151

Senohrabkova L., Malcova I, Hasek J. (2019)An aggregation-prone mutant of eIF3a forms reversible assemblies escaping spatial control in exponentially growing yeast cells. Curr Genet. (2019) 65:919–940

Binarová P., Tuszynski J. (2019)Tubulin: Structure, Functions and Roles in Disease Cells (2019) 8:1294

Chumová J., Kourová H., Trögelová L., Halada P., Binarová P. (2018)Microtubular and Nuclear Functions of γ-Tubulin: Are They LINCed? Cells (2019) 8:259

Breitenbach M., Rinnerthaler M., Weber M., Breitenbach-Koller H., Karl T.,Cullen P., Basu S., Haskova D., Hasek J. (2018)The defense and signaling role of NADPH oxidases in eukaryotic cells. Wien Med Wochenschr (2018) 168:286–299

Chumová J., Trögelová L., Kourová H., Volc J., Sulimenko V., Halada P., Kučera O., Benada O., Kuchařová A., Klebanovych A., Dráber P., Daniel G., Binarová P. (2018) γ-Tubulin has a conserved intrinsic property of self-polymerization into double stranded filaments and fibrillar networks. Biochim Biophys Acta (2018) 2018 May;1865(5):734-748. doi: 10.1016/j.bbamcr.2018.02.009. Epub 2018 Feb 27.

Bischof J., Salzmann M., Streubel M.K., Hasek J., Geltinger F., Duschl J., Bresgen N., Briza P., Haskova D., Lejskova R., Sopjani M., Richter K., Rinnerthaler M. (2017) Clearing the outer mitochondrial membrane from harmful proteins via lipid droplets. Cell Death Discovery (2017) 3, 17016; doi:10.1038/cddiscovery.2017.16.

Zhang T., Galdieri L.,Hasek J., Vancura A. (2017)Yeast phospholipase C is required for stability of casein kinase I Yck2p and expression of hexose transporters. FEMS Microbiol Lett. 364(22). doi: 10.1093/femsle/fnx227

Horvath, B., Kourova, H., Nagy S., Nemeth S., Magyar Z., Papdi C., Ahmad Z., Sanchez-Perez G., Perilli S., Blilou I., Meszaros T., Binarova P., Bogre L., Scheres B. (2017) Arabidopsis RETINOBLASTOMA RELATED regulates DNA damage response independently of the cell-cycle.EMBO Journal 2017 May 2;36(9):1261-1278. doi: 10.15252/embj.201694561. Epub 2017 Mar 20.

Vasicova P., Rinnerthaler M., Haskova D., Novakova L., Malcova I., Breitenbach M., Hasek J. (2016) Formaldehyde fixation is detrimental to actin cables in glucose-depleted S.cerevisiae cells. Microbial Cell 2016 Apr 12;3(5):206-214. doi: 10.15698/mic2016.05.499.

Malcova I., Farkasovsky M.,Senohrabkova L., Vasicova P., Hasek J. (2016) New integrative modules for multicolor-protein labeling and live-cell imaging in Saccharomyces cerevisiae. FEMS Yeast Research, 16, doi: 10.1093/femsyr/fow027

Wang X., Xi W., Toomey S., Chiang Y-C.,Hasek J., Laue T.M., et al. 2016) Stoichiometry and Change of the mRNA Closed-Loop Factors as Translating Ribosomes Transit from Initiation to Elongation. PLoS ONE 11(3): e0150616. doi:10.1371/

Vasicova P., Lejskova R., Malcova I., Hasek J. (2015) The stationary phase cells of S. cerevisiae display dynamic actin filaments required for processes extending chronological life span . Mol. Cell. Biol. 35, 3892-3908.

Grousl T., Opekarova M., Stradalova V., Hasek J., Malinsky J.(2015) Evolutionarily conserved 5'-3' exoribonuclease Xrn1 accumulates at plasma membrane-associated eisosomes in post-diauxic yeast. PLoS ONE 10, e0122770.

Kohoutová, L., Kourová, H., Nagy S., Volc, J., Halada,P., Mészáros, T., Meskiene, I., Bögre, L., Binarová, P. (2015). The Arabidopsis mitogen-activated protein kinase 6 is associated with γ-tubulin on microtubules, phosphorylates EB1c and maintains spindle orientation under nitric oxide stress. New Phytol. 207(4), 1061-74.

Rinnerthaler M., Lejskova R., Grousl T.,Stradalova V., Heeren G., Richter K., Breitenbach-Koller L., Malinsky J., Hasek J., Breitenbach M. (2013) Mmi1, the yeast homologue of mammalian TCTP, associates with stress granules in heat-shocked cells and modulates proteasome activity.PLoS ONE 8, e77791.

Malinsky J., Opekarova M., Grossmann G., Tanner W. (2013) Membrane Microdomains Rafts, and Detergent-Resistant Membranes in Plants and Fungi. Annu. Rev. Plant. Biol. 64, 501-529. doi:10.1146/annurev-arplant-050312-120103

Grousl T., Ivanov P., Malcova I., Pompach P., Frydlova I., Slaba R., Senohrabkova L., Novakova L., Hasek J. (2013) Heat shock-induced accumulation of translation elongation and termination factors precedes assembly of stress granules in S. cerevisiae. PLoS ONE 8, e57083.

Gaur N.A., Hasek J., Brickner D.G., Qiu H., Zhang F., Wong C.M., Malcova I., Vasicova P., Brickner J.H., Hinnebusch A.G. (2013)Vps factors are required for efficient transcription elongation in budding yeast. Genetics 193, 829–851

Petrovská,B. Jeřábková, H., Kohoutová, L.,Cenklová, V., Pochylová, Z., Gelová, Z., Kočárová, G., Váchová, L., Kurejová, M., Tomaštíková, E., Binarová. P. (2013). Overexpressed TPX2 causes ectopic formation of microtubular arrays in the nuclei of acentrosomal plant cells. J. Exp. Bot. 64 (14), 4575–4587.

Doskočilová, A., Kohoutová L., Volc, J., Kourová, H., Benada O., Chumová, J., Plíhal O., Petrovská, B., Halada P., Bogre L., Binarova P. (2013). Nitrilase 1 regulates the exit from proliferation, genome stability and plant development. New Phytol.198, 685-698.

Vasicova P., Stradalova V., Halada P., Hasek J., Malcova I. (2013) Nuclear import of chromatin remodeler Isw1 is mediated by atypical bipartite cNLS and classical import pathway. Traffic 14,176-93. doi: 10.1111/tra.12025. Epub 2012 Nov 26.

Rinnerthaler M., Büttner S., Laun P., Heeren G., Felder T.K., Klinger H., Weinberger M., Stolze K., Grousl T., Hasek J., Benada O., Frydlova I., Klocker A., Simon-Nobbe B., Jansko B., Breitenbach-Koller H., Eisenberg T., Gourlay C.W., Madeo F., Burhans W.C., Breitenbach M. (2012) Yno1p/Aim14p, a NADPH-oxidase ortholog, controls extramitochondrial reactive oxygen species generation, apoptosis, and actin cable formation in yeast Proc. Natl. Acad. Sci. USA 29, 8658-8663