Valášek, L.S. (2012) Ribozoomin’ – Translation Initiation from the Perspective of the ribosome-bound Eukaryotic Initiation Factors (eIFs). Curr Protein Pept Sci., 9, in press. LINK

Kouba, T., Rutkai, E., Karaskova, M., and Valášek, L.S. (2012) The eIF3c/NIP1 PCI domain interacts with RNA and RACK1/ASC1 and promotes assembly of the pre-initiation complexes. Nucleic Acid Res., 40, in press. LINK

Herrmannová, A., Daujotyte, D., Ji-Chun, Y., Cuchalová, L., Gorrec, F., Wagner, S., Dányi, I., Lukavsky, P.J., and Valášek, L.S. (2012) Structural analysis of an eIF3 subcomplex reveals conserved interactions required for a stable and proper translation pre-initiation complex assembly. Nucleic Acid Res., 40, 2294-311. LINK

Munzarová, V., Pánek, J., Gunišová, S., Dányi, I., Szamecz, B., and Valášek, L.S. (2011) Translation reinitiation relies on the interaction between eIF3a/TIF32 and progressively folded cis-acting mRNA elements preceding short uORFs. PLoS Genet., 7(7), e1002137. LINK

Masek, T., Valášek, L., and Pospisek, M. (2011) Polysome Analysis and RNA Purification from Sucrose Gradients. Methods Mol Biol., 703, 293-309. LINK

Nemoto, N., Singh, C.R., Udagawa, T., Wang, S., Thorson, E., Winter, Z., Ohira, T., Ii, M., Valášek, L., Brown, S.J., and Asano, K. (2010) Yeast 18S rRNA is directly involved in the ribosomal response to stringent AUG selection during translation initiation. J Biol Chem., 285, 32200-12. LINK

Cuchalová, L., Kouba, T., Herrmannová, A., Dányi, I., Chiu, W.L., and Valášek, L. (2010) The RNA recognition motif of eukaryotic translation initiation factor 3g (eIF3g) is required for resupmtion of scanning of post-termination ribosomes for reinitiation on GCN4 and together with eIF3i stimulates linear scanning. Mol. Cell Biol., 19, 4671-86. LINK

Chiu, W.L., Wagner, S., Herrmannová, A., Burela, L., Zhang, F., Saini, A.K., Valášek, L., and Hinnebusch A.G. (2010) The C-terminal region of eukaryotic translation initiation factor 3a (eIF3a) promotes mRNA recruitment, scanning, and, together with eIF3j and the eIF3b RNA recognition motif, selection of AUG start codons. Mol. Cell Biol., 18, 4415-34. LINK

ElAntak, L., Wagner, S., Herrmannová, A., Karásková, M., Rutkai, E., Lukavsky, P.J., and Valášek, L. (2010) The indespensable N-terminal half of eIF3j co-operates with its structurally conserved binding partner eIF3b-RRM in strigent AUG selection. J. Mol. Biol., 396, 1097-116. LINK

Grousl, T., Ivanov, P., Frydlova, I. Vasicova, P., Janda, F., Vojtova, J., Malinska, K., Malcova, I., Novakova, L., Janoskova, D., Valášek, L., and Hasek, J. (2009) Robust heat shock induces eIF2a-phosphorylation-independent assembly of stress granules containing eIF3 and 40S ribosomal subunits in budding yeast S. cerevisiae. J. Cell Sci., 122, 2078-88. LINK

Szamecz, B., Rutkai, E., Cuchalová, L., Munzarová, V., Herrmannová, A., Nielsen, K.H., Burela, L., Hinnebusch, A.G., and Valášek, L. (2008) eIF3a cooperates with sequences 5' of uORF1 to promote resupmtion of scanning by post-termination ribosomes for reinitiation on GCN4 mRNA. Genes & Dev., 22, 2414-2425. LINK

Valášek, L., Szamecz, B., Hinnebusch, A.G., and Nielsen, K.H. (2007) In vivo stabilization of pre-initiation complexes by formaldehyde cross-linking. Methods Enzymol., 429, 163-183. LINK

Nielsen, K.H. and Valášek, L. (2007) In vivo deletion analysis of the architecture of a multi-protein complex of transaltion initiation factors. Methods Enzymol., 431, 15-32. LINK

Nielsen, K.H., Valášek, L., Sykes, C., Jivotovskaya, A.V., and Hinnebusch, A.G. (2006) Interaction of the RNP1 motif in PRT1 with HCR1 promotes 40S binding of eukaryotic initiation factor 3 in yeast. Mol. Cell Biol., 26, 2984-98. LINK

Jivotovskaya, A.V., Valášek, L., Hinnebusch, A.G., and Nielsen, K.H. (2006) Eukaryotic translation initiation factor 3 (eIF3) and eIF2 can promote mRNA binding to 40S subunits independently of eIF4G in yeast. Mol. Cell Biol., 26, 1355-72. LINK

Hašek, J., Peřinka, L., and Valášek, L. (2005) Specification of the monoclonal antibody PK1 reactivity in Chinese hamster ovary cells. Folia Biol., 51, 50-1. LINK

Hinnebusch, A.G., Asano, K., Olsen, D.S., Phan, L., Nielsen, K.H., and Valášek, L. (2004) Study of Translational Control of Eukaryotic Gene Expression Using Yeast. Ann N Y Acad Sci. 1038, 60-74. LINK

Valášek, L., Nielsen, K.H., Zhang, F., Hamilton, A.C., and Hinnebusch, A.G. (2004)  Interactions of eIF3 subunit NIP1/c with eIF1 and eIF5 promote pre-initiation complex assembly and regulate start codon selection. Mol. Cell Biol., 24, 9437-9455. LINK

Valášek, L., Mathew, A.A., Shin, B-S., Nielsen, K.H., Szamecz, B., and Hinnebusch, A.G. (2003) The Yeast eIF3 Subunits TIF32/a and NIP1/c and eIF5 Make Critical Connections with the 40S Ribosome in vivo. Genes & Dev 14, 2534-2546. LINK

Valášek, L., Nielsen, K.H., and Hinnebusch, A.G. (2002) Direct eIF2-eIF3 Contact in the Multifactor Complex is Important for Translation Initiation in vivo. EMBO J., 21, 5886-98. LINK

Valášek, L., Hašek, J., Nielsen, K.H., and Hinnebusch, A.G. (2001) Dual function of eIF3j/Hcr1p in processing 20S pre-rRNA and translation initiation. J. Biol. Chem., 276, 43351-43360. LINK

Valášek, L., Phan, L., Schoenfeld, L.W., Valášková, V., and Hinnebusch, A.G. (2001) Related eIF3 subunits TIF32 and HCR1 interact with an RNA recognition motif in PRT1 required for eIF3 integrity and ribosome binding. EMBO J., 20, 891-904. LINK

Valášek, L., Hašek, J., Trachsel, H., Imre, E.I., and Ruis, H. (1999) The Saccharomyces cerevisiae HCR1 gene encoding a homologue of the p35 subunit of human translation initiation factor 3 (eIF3) is a high copy suppressor of a temperature-sensitive mutation in the Rpg1p subunit of yeast eIF3. J. Biol. Chem., 274, 27567-27572. LINK

Valášek, L., Trachsel, H., Hašek, J., and Ruis, H., (1998) Rpg1, the Saccharomyces cerevisiae homologue of the largest subunit of mammalian translation initiation factor 3, is required for translational activity. J. Biol. Chem., 273, 21253-21260. LINK

Shalev, A., Valášek, L., Pise-Masison, C.A., Radonovich, M., Phan, L., Clayton, J., Brady, J.N., Hinnebusch, A.G, and Asano, K. (2001) Saccharomyces cerevisiae protein PCI8 and human protein eIF3e/Int-6 interact with eIF3 core complex by binding to cognate eIF3b subunits. J Biol Chem., 276, 34948-57. LINK

Nielsen, K.H., Szamecz, B., Valášek, L., Jivotovskaya, A., Shin, B-S., and Hinnebusch, G.H. (2004) Yeast eIF3 has critical functions downstream of 48S assembly that impact AUG recognition and GCN4 translational control. EMBO J., 23, 1166-77. LINK

Phan, L., Shoenfeld, L.W., Valášek, L., Nielsen, K.H., Olsen, D., and Hinnebusch, G.H. (2001) A subcomplex of three eIF3 subunits binds eIF1 and eIF5 and stimulates ribosome binding of mRNA and tRNA(i)Met. EMBO J., 20, 2954-65. LINK

Asano K., Phan, L., Valášek, L., Schoenfeld, L.W., Shalev, A., Clayton, J., Nielsen, K., Donahue, T.F., and Hinnebusch A.G. A Multifactor Complex of eIF1, eIF2, eIF3, eIF5, and tRNAiMet Promotes Initiation Complex Assembly and Couples GTP Hydrolysis to AUG Recognition (2001) Cold Spring Harbor Symposia on Quantitative Bilology, Vol. LXVI, 403-415, CSHL Press. LINK

Hašek, J., Kovarik, P., Valášek, L., Malínská, K., Schneider J., Kohlwein S.D., and Ruis, H., (2000) Rpg1p, the subunit of the Saccharomyces cerevisiae eIF3 core complex, is a microtubule-interacting protein. Cell Motil. Cytoskel., 45, 235-46. LINK

Sabelli, P.A., Burgess, S.R., Valášek, L., and Shewry, P.R. (1998) Molecular cloning and characterization of a maize cDNA for a homologue of the large subunit of the eukaryotic initiation factor 3 (eIF3). Mol Gen Genet., 261, 820-830. LINK

Kovarik, P., Hašek, J., Valášek, L., and Ruis, H., (1998) RPG1: an essential gene of  Saccharomyces cerevisiae encoding a 110 kDa protein required for passage through the G1 phase. Curr. Genetics, 33, 100-109. LINK

Asano, K., Shalev, A., Phan, L., Nielsen, K., Clayton, J., Valášek, L., Donahue, T., and Hinnebusch A.G. (2001) Multiple roles for the C-terminal domain of eIF5 in translation initiation complex assembly and GTPase activation. EMBO J., 20, 2326-37. LINK

Vondrejs, V., Janderova, B, Valášek, L. (1996) Yeast killer toxin K1 and its exploitation in genetic manipulations. Folia Microbiol., 41, 379-93.

Vondrejs, V., Valášek, L. (1994) Comparison of different variants of the rhodamine test in terms of sensitivity of detection of the effect of zymocins in different strains of Saccharomyces cerevisiae. Folia Microbiol., 39, 4, 1, 0015-5632.

Vondrejs, V., Valášek, L. (1994) Comparison of the effect of zymocine K1 on protoplasts and cells of selected yeast species. Folia Microbiol., 39, 4, 1, 0015-5632.