SHORT COMMUNICATION
The Effect of Goldthioglucose
on Peroxidative Processes in Mice
D. KOŠŤOVÁ, E. MICHNOVÁ
Department of Pathophysiology, University of
Veterinary Medicine, Košice, Slovak Republic
Received May 28, 1998
Accepted October 27, 1998
Summary
Experiments were conducted to study the effect of
goldthioglucose (GTG) upon the processes associated with lipid
peroxidation. The glucose-6-phosphate dehydrogenase activity
(G6PD; E.C.1.1.1.49) in red blood cells (RBC) and the amount of
malonaldehyde precursors (MDA) per gram of brain, liver and
kidney were determined. Adult mice received i.p. injections for
three consecutive days of either saline (controls) or GTG
dissolved in saline, in a dose of 0.10 mg.g-1 or 0.15 mg.g-1
b.w. In mice receiving higher dose of GTG the G6PD activity was
significantly increased (349.38± 17.46 mU.10-9 RBC compared to
258.2± 14.46 mU.10-9 RBC in control animals). The content of MDA
precursors rose significantly from 4.8± 0.81 m mol.g-1 of the
liver in controls to 8.12± 1.41 m mol.g -1 and 7.88± 0.51 m
mol.g-1 and from 18.71± 1.01 m mol.g-1 of the kidneys in
controls to 24.25± 1.25 m mol.g-1 and 24.88± 1.7 m mol.g-1
respectively. The GTG-induced higher levels of MDA precursors
and increased G6PD activity in RBC corresponds to the rise in
lipid peroxidation and its participation in producing the
lesions after experimental and therapeutic use of
gold-containing substances seems possible.
Key words
Goldthioglucose - Glucose-6-phosphate dehydrogenase -
Malonaldehyde precursors - Mice
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