Effects of Histamine H1
Antagonist Dithiaden on Acetic Acid-Induced Colitis in Rats
V. NOSÁĽOVÁ, O. ONDREJIČKOVÁ, J. PEČIVOVÁ
Institute of Experimental Pharmacology, Slovak Academy of
Sciences, Bratislava, Slovak Republic
Received June 24, 1998
Accepted October 4, 1998
Summary
To assess the possible involvement of mast cells and/or their
mediators in inflammatory bowel diseases, the effect of the
histamine H1 antagonist Dithiaden was studied on a model of
acetic acid-induced colitis in rats. Dithiaden pretreatment by
intracolonic administration was found to reduce the extent of
acute inflammatory colonic injury. This was manifested by a
decrease in the score of gross mucosal injury, by lowered
colonic wet weight and by diminished myeloperoxidase activity
reflecting reduced leukocyte infiltration. Vascular permeability
and gamma-glutamyl transpeptidase activity, elevated by acetic
acid exposure, were decreased after Dithiaden pretreatment. The
results indicate that locally administered Dithiaden may protect
the colonic mucosa against an acute inflammatory attack by
interfering with the action of the major mast cell mediator
histamine.
Key words
Dithiaden – Acetic acid-induced colitis – Histamine – Mast cells
– Rats
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