Selective Antioxidant Enzymes during Ischemia/Reperfusion in Myocardial
Infarction
V. MUŽÁKOVÁ, R. KANĎÁR, P. VOJTÍŠEK1,
J. SKALICKÝ2, Z. ČERVINKOVÁ3
Department of Biological and Biochemical Sciences, Faculty of Chemical
Technology, University of Pardubice, 1Department of Cardiology,
Hospital of Pardubice, 2Department of Clinical Biochemistry, Hospital
of Pardubice, 3Department of Physiology, Faculty of Medicine, Charles
University, Hradec Králové, Czech Republic
Received September 9, 1999
Accepted December 21, 1999
Summary
The study of ischemia/reperfusion injury included 25 patients
in the acute phase of myocardial infarction (19 perfused, 6 remained non-reperfused
as evaluated according to the time course of creatine kinase and CK-MB isoenzyme
activity) and a control group (21 blood donors). Plasma level of malondialdehyde
was followed as a marker of oxidative stress. Shortly after reperfusion (within
90 min), a transient increase of malondialdehyde concentration was detected. The
return to the baseline level was achieved 6 h after the onset of therapy. The
activity of a free radical scavenger enzyme, plasma glutathione peroxidase (GPx),
reached its maximum 90 min after the onset of treatment and returned to the
initial value after 18 h. The specificity of the GPx response was confirmed by
comparing with both non-reperfused patients and the control group, where no
significant increase was detected. The erythrocyte Cu,Zn-superoxide dismutase
(SOD) did not exhibit significant changes during the interval studied in perfused patients, probably due to the stability of erythrocyte metabolism. In
non-reperfused patients, a decrease of SOD was found during prolonged hypoxia.
These results help to elucidate the mechanisms of fast activation of plasma
antioxidant system during the reperfusion after myocardial infarction.
Key words
Superoxide dismutase ·
Glutathione peroxidase · Malondialdehyde ·
Myocardial infarction · Oxidative stress
Reprint requests
V. Mužáková, M.D.,
Department of Biological and Biochemical Sciences, Faculty of Chemical
Technology, University of Pardubice, Štrossova 239, 530 03 Pardubice, Czech
Republic.
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