Physiol. Res. 49: 733-736, 2000 |
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SHORT
COMMUNICATION
Ozone-Induced
Micronuclei Frequency in Rat Alveolar Type II
Cells
D.
CHORVATOVIČOVÁ, P. H. M. HOET1, E. TÁTRAI2,
Z. KOVÁČIKOVÁ3
Institute
of Experimental Pharmacology, Slovak Academy of
Sciences, Bratislava, Slovak Republic,1Katholieke
Universiteit, Leuven, Belgium, 2National
Institute of Occupational Health, Budapest,
Hungary and 3Institute
of Preventive and Clinical Medicine, Bratislava,
Slovak Republic
Received
November 16, 1999
Accepted March 10, 2000
Summary
The
effect of ozone, a ubiquitous air pollutant, was
tested on cultured pulmonary epithelial type II
cells isolated from rats. After 40-hour culture,
the cells were exposed for 6 h to 400 ppb of
ozone or air. The number of micronucleated cells
was counted after the exposure. In each group, 17
000 cells were evaluated. The number of micronucleated cells was significantly increased
in the ozone-exposed group (12.24 per 1000 cells)
compared to the control group (5.00 per 1000
cells). The results showed the mutagenic effect
of ozone exposure on alveolar type II cells,
manifested in the increased frequency of their
micronuclei.
Key words
Micronuclei
· Alveolar epithelial type II cells · Ozone ·
In vitro
Reprint
requests
Z. Kováčiková, Institute of Preventive and
Clinical Medicine, Limbová 14, 833 01
Bratislava, Slovak Republic. Fax:
+421-7-54773906, e-mail: kovacik@upkm.sk
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