Physiol. Res. 49: 673-678, 2000

Somatotropin has no Effect on the Quantity of Guanine Nucleotide Binding Proteins Gqalpha/G11alpha in Goat Adipose Tissue in vivo


Institute of Animal Physiology and Genetics, Academy of Sciences of the Czech Republic, 1Research Psychiatric Unit, First Faculty of Medicine, Charles University, 2Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic and 3Department of Veterinary Services, National Institute of Public Health, Prague, Czech Republic

Received October 12, 1998
Accepted April 18, 2000

The decapeptide QLNLKEYNLV corresponding to the C-terminus of Gq/G11alpha guanine nucleotide-binding proteins (G-proteins) was synthesized by the solid-phase method and conjugated to keyhole limpet hemocyanin. The rabbits were immunized with these conjugates and an antiserum that reacted specifically with the alpha subunit of Gq/G11 proteins was used in this study. The antiserum exhibited no cross-reactivity with the alpha subunits of stimulatory (Gs) or inhibitory (Gi) G-proteins associated with adenylate cyclase. Immunoblots with the antiserum showed that it specifically recognized the Gq/G11 alpha-proteins in cholate extracts of adipose tissue membranes of goats. Treatment of young castrated male goats with bST had no effect on the quantity of Gq/G11 alpha-subunits in adipose tissue and the results thus obtained did not support the idea that the bST signal in adipose tissue is transmitted via Gq/G11 alpha-proteins.

Key words
Adipose tissue · Goat · Somatotropin · G-proteins

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© 2000 by the Institute of Physiology, Czech Academy of Sciences