Somatotropin has
no Effect on the Quantity of Guanine Nucleotide
Binding Proteins Gqalpha/G11alpha
in Goat Adipose Tissue in vivo
V. KRBEČEK,
H. KOVÁŘŮ1, J. ŠKARDA, T. BARTH2,
J. VELEK2, V. ŽIŽKOVSKÝ3
Institute
of Animal Physiology and Genetics, Academy of
Sciences of the Czech Republic, 1Research
Psychiatric Unit, First Faculty of Medicine,
Charles University, 2Institute
of Organic Chemistry and Biochemistry, Academy of
Sciences of the Czech Republic and 3Department
of Veterinary Services, National Institute of
Public Health, Prague, Czech Republic
Received
October 12, 1998
Accepted April 18, 2000
Summary
The
decapeptide QLNLKEYNLV corresponding to the
C-terminus of Gq/G11alpha guanine
nucleotide-binding proteins (G-proteins) was
synthesized by the solid-phase method and
conjugated to keyhole limpet hemocyanin. The
rabbits were immunized with these conjugates and
an antiserum that reacted specifically with the
alpha subunit of Gq/G11 proteins was used in this
study. The antiserum exhibited no
cross-reactivity with the alpha subunits of
stimulatory (Gs) or inhibitory (Gi) G-proteins
associated with adenylate cyclase. Immunoblots
with the antiserum showed that it specifically
recognized the Gq/G11 alpha-proteins in cholate
extracts of adipose tissue membranes of goats.
Treatment of young castrated male goats with bST
had no effect on the quantity of Gq/G11 alpha-subunits
in adipose tissue and the results thus obtained
did not support the idea that the bST signal in
adipose tissue is transmitted via Gq/G11 alpha-proteins.
Key words
Adipose
tissue · Goat · Somatotropin · G-proteins
Reprint
requests
J.
Škarda, Institute of Animal Physiology and
Genetics, Academy of Sciences of the Czech
Republic, 104 00 Prague 10 - Uhříněves, Czech
Republic. E-mail: skarda@iapg.cas.cz
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