Ontogenetic
Profile of Ecto-ATPase Activity in Rat
Hippocampal and Caudate Nucleus Synaptic Plasma
Membrane Fractions
A. BANJAC, N.
NEDELJKOVIĆ, A. HORVAT, D. KANAZIR, G. NIKEZIĆ
Department
of Molecular Biology and Endocrinology, Vinča
Institute of Nuclear Sciences, Belgrade,
Yugoslavia
Received July 10, 2000
Accepted January 4, 2001
Summary
An
ontogenetic study of ecto-ATPase activity and the
content of enzyme proteins was assessed in the
caudate nucleus and hippocampal synaptic plasma
membranes isolated from rats at various ages (15,
30, 90, 180 and 365 days). The ontogenetic
profile revealed that the enzyme activities in
both brain areas were the highest on day 30 and
365, while the ecto-ATPase protein abundance was
the highest on day 15 after birth. Possible
explanation for obtained ontogenetic profile and
the discrepancy between activity and abundance
may reside in the fact that ecto-ATPase during
development could exert additional roles other
than those related to metabolism of ATP. It is
likely that ecto-ATPase, regulating the
concentration of ATP and adenosine in synaptic
cleft, has important role in the processes of
brain development and aging.
Key
words
Ecto-ATPase
· Extracellular purines · Development · Aging
· Brain
Reprint
requests
A.
Horvat, Department of Molecular Biology and
Endocrinology, Vinča Institute of Nuclear
Sciences, P.O. Box 522, 11001 Belgrade,
Yugoslavia. Phone 381 11 344 36 19. E-mail: ahorvat@rt270.vin.bg.ac.yu
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