Physiol. Res. 51: 49-58, 2002

Multinuclear NMR Studies of an Actively Dividing Artificial Tumor

E. G. SHANKLAND1, J. C. LIVESEY4, R. W. WISEMAN1,3, K. A. KROHN1,2

1Department of Radiology and 2Department of Radiation Oncology, 3Department of Biochemistry, University of Washington, Seattle, Washington, 4Department of Physiology and Pharmacology, University of the Pacific, Stockton, California, USA

Received November 10, 2000
Accepted June 6, 2001


Summary
Growth of the A549 cell line in a perfusion system suitable for use in a magnetic resonance study has been characterized and shown to be stable physiologically and hence appropriate for serial observations. Several methods of monitoring cell growth were compared to assess the behavior of the cells in this system. Comparison between NMR metabolite data and cell growth via cell counting showed that 31P NMR signals accurately reported cell doubling time. In contrast to most NMR cell culture systems, viable cells can be recovered from the perfusion system after the NMR measurements for further biochemical studies. These data further suggest that this system will be useful for studying the physiology and biochemistry of exponentially growing cells for at least two days in NMR tube culture.


Key words
A549 Phophorous NMR Cell culture

Reprint requests
E. Shankland, Dept. of Radiology, box 357115, University of Washington, Seattle, Washington 98195, USA, e-mail: shanklan@u.washington.edu


2002 by the Institute of Physiology, Czech Academy of Sciences