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Multinuclear NMR Studies of an
Actively Dividing Artificial Tumor
E. G. SHANKLAND1, J.
C. LIVESEY4, R. W. WISEMAN1,3, K. A. KROHN1,2
1Department of Radiology and 2Department
of Radiation Oncology, 3Department of Biochemistry,
University of Washington, Seattle, Washington, 4Department
of Physiology and Pharmacology, University of the Pacific,
Stockton, California, USA
Received November 10, 2000
Accepted June 6, 2001
Summary
Growth of the A549 cell line in a perfusion system suitable for use in a
magnetic resonance study has been characterized and shown to be stable
physiologically and hence appropriate for serial observations. Several methods
of monitoring cell growth were compared to assess the behavior of the cells in
this system. Comparison between NMR metabolite data and cell growth via cell
counting showed that 31P NMR signals accurately reported cell
doubling time. In contrast to most NMR cell culture systems, viable cells can be
recovered from the perfusion system after the NMR measurements for further
biochemical studies. These data further suggest that this system will be useful
for studying the physiology and biochemistry of exponentially growing cells for
at least two days in NMR tube culture.
Key words
A549 · Phophorous · NMR · Cell culture
Reprint requests
E. Shankland, Dept. of Radiology, box 357115, University of
Washington, Seattle, Washington 98195, USA, e-mail:
shanklan@u.washington.edu
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