SHORT
COMMUNICATION
Cytosolic
Free Ca2+
Concentration in Canine Aortic Endothelial Cells
Lining the Polyester Arterial Prosthesis
P.
ONDRU1, R. ALBERTY2, P.
POTHIER3, V. ECHAVÉ4, J. POISSON4, G. BKAILY3
1Department of
Cardiac Surgery, F. D. Roosevelt Hospital, 2Department
of Biology, Faculty of Science, Matthias Belius
University, Banská Bystrica, Slovakia, 3Department
of Physiology and Biophysics, Faculty of
Medicine, University of Sherbrooke and 4Department
of Surgery, Sherbrooke University Medical Center,
Sherbrooke, Quebec, Canada
Received March 12, 2001
Accepted August 2, 2001
Summary
A rise in baseline cytosolic free Ca2+
in canine vascular endothelial-like cells (VEC)
lining the luminal surface of the polyester
arterial prosthesis is described. In one, three
and six month implantation experiments we
employed six adult mongrel dogs, polyester
arterial prostheses Arteknit Ra K, fluorescent
Ca2+ indicator Fura-2 and digital imaging
microscopy to study cytosolic free Ca2+ in
cultured VEC. The electron microscopy scanning of
the luminal surface in different regions of the
graft were also performed. A rise in cytosolic
free Ca2+ in the VEC lining the luminal surface
of the prosthesis is probably the result of the
immunologic reaction and mechanical stress which
stimulate the proliferation activity of the
endothelial cells. It seems that the baseline
cytosolic free Ca2+ reflects the course of the
endothelization process on the polyester arterial
prosthesis..
Key
words
Ionized
calcium ˇ Endothelial cells ˇ Arterial
prosthesis ˇ Canine model
Reprint
requests
Dr. Roman Alberty, Department of Biology, Faculty
of Science, Matthias Belius University,
Tajovského 40, 974 01 Banská Bystrica,
Slovakia. phone: + 00421 48 413 98 19. e-mail: alberty@fpv.umb.sk
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