Volume 51: 217-220, 2002


SHORT COMMUNICATION


Cytosolic Free Ca2+ Concentration in Canine Aortic Endothelial Cells Lining the Polyester Arterial Prosthesis


P. ONDRUŠ1, R. ALBERTY2, P. POTHIER3, V. ECHAVÉ4, J. POISSON4, G. BKAILY3

1Department of Cardiac Surgery, F. D. Roosevelt Hospital, 2Department of Biology, Faculty of Science, Matthias Belius University, Banská Bystrica, Slovakia, 3Department of Physiology and Biophysics, Faculty of Medicine, University of Sherbrooke and 4Department of Surgery, Sherbrooke University Medical Center, Sherbrooke, Quebec, Canada


Received March 12, 2001
Accepted August 2, 2001


Summary
A rise in baseline cytosolic free Ca2+ in canine vascular endothelial-like cells (VEC) lining the luminal surface of the polyester arterial prosthesis is described. In one, three and six month implantation experiments we employed six adult mongrel dogs, polyester arterial prostheses Arteknit Ra K, fluorescent Ca2+ indicator Fura-2 and digital imaging microscopy to study cytosolic free Ca2+ in cultured VEC. The electron microscopy scanning of the luminal surface in different regions of the graft were also performed. A rise in cytosolic free Ca2+ in the VEC lining the luminal surface of the prosthesis is probably the result of the immunologic reaction and mechanical stress which stimulate the proliferation activity of the endothelial cells. It seems that the baseline cytosolic free Ca2+ reflects the course of the endothelization process on the polyester arterial prosthesis..


Key words
Ionized calcium ˇ Endothelial cells ˇ Arterial prosthesis ˇ Canine model

Reprint requests
Dr. Roman Alberty, Department of Biology, Faculty of Science, Matthias Belius University, Tajovského 40, 974 01 Banská Bystrica, Slovakia. phone: + 00421 48 413 98 19. e-mail:
alberty@fpv.umb.sk


PHYSIOLOGICAL RESEARCH
Š 2002 by the Institute of Physiology, Czech Academy of Sciences

ISSN 0862 - 8408

Issue 2