Cardiac Troponin T Following Repeated Administration of Pyridoxal Isonicotinoyl Hydrazone in Rabbits

M. ADAMCOVÁ, J. MACHÁČKOVÁ, V. GERŠL, V. PELOUCH¹, T. ŠIMŮNEK², I. KLIMTOVÁ², R. HRDINA², P. POŇKA³
 

Charles University in Prague, Faculty of Medicine in Hradec Králové, ²Faculty of Pharmacy in Hradec Králové, ¹Second Faculty of Medicine in Prague, Czech Republic, ³McGill University, Montreal, Canada
 

Received April 6, 2001
Accepted April 5, 2002
 

Summary
Pyridoxal isonicotinoyl hydrazone (PIH) is a new tridentate Fe-chelating agent that should be very promising in many pathological states resulting from both an iron-overload and formation of free radicals. The aim of our study was to investigate the effect of PIH on the cardiovascular system focusing to the regulatory protein - cardiac troponin T (cTnT). The study was carried out in two groups of Chinchilla male rabbits: 1) PIH (50 mg/kg dissolved in 10 % Cremophor i.p., once a week, 10 administrations, n=8) and 2) Cremophor (2 ml/kg i.p. in the same schedule, n=7). Plasma concentrations of cTnT (as a marker of myocardial damage) were measured using a commercial kit (Roche). cTnT was within the physiological range (i.e. < 0.1 mg/l) during the whole experiment in the Cremophor group. In the PIH group, the cTnT levels were not significantly increased when compared with the control group or with the initial values (except with those before the 5th administration). Furthermore, we analyzed the cytosolic and myofibrillar fraction of cTnT in the left ventricular myocardium. Using SDS-PAGE and Western blot we resolved three isoforms. The profiling of TnT did not differ significantly between the PIH-treated group and the Cremophor-treated group. Our data concerning cTnT support the opinion that the possible cardiotoxicity of PIH is very low.
 

Key words
Iron chelators · Cardiotoxicity · Cardiac troponin T · Protein profile · Rabbits
 

Reprint requests
MUDr. M. Adamcová, CSc., Department of Physiology, Faculty of Medicine of Charles University, Šimkova 870, CZ-50001 Hradec Králové. E-mail: adamcova@lfhk.cuni.cz
 

ISSN 0862 - 8408