Physiol. Res. 51: 493-499, 2002


Lidocaine-Containing Euro-Colins Solution Prevents Renal Injury in the Isolated Perfused Canine Kidney Exposed to Prolonged Cold Ischemia
 

N. ERKASAP, E. ATES1, S. ERKASAP1, Z. KAYGISIZ
 

Department of Physiology and 1Department of General Surgery, Osmangazi University, Medical Faculty, Eskisehir, Turkey
 

Received October, 19, 2001
Accepted January 21, 2002
 


Summary
Previous studies have reported a decreased incidence of delayed graft function after cadaveric transplantation with the use of lidocaine pretreatment of the donor. We evaluated the effects of lidocaine on prolonged cold ischemia and reperfusion injury in a canine model of isolated kidney perfusion (IPK). The purpose of this study was to evaluate the renal function of isolated perfused canine kidneys after 48 h of cold storage with Euro-Collins (EC) solution or EC solution plus lidocaine. Isolated perfused canine kidneys were randomized into four groups which contained six kidneys: I) cold flush with EC solution and immediately reperfused, II) cold flush with EC solution plus lidocaine and immediately reperfused, III) 48 h of cold storage with EC and reperfusion, IV) 48 h of cold storage with EC solution plus lidocaine and reperfusion. The measured renal functions were glomerular filtration rate, urine production, perfusate flow, urinary lactic dehydrogenase (ULDH), Na reabsorptive capacity, and tissue MDA levels. Histological examination was performed after reperfusion. The tubular functions of kidneys preserved with EC solution containing lidocaine were better when compared with the kidneys preserved with EC alone. Tubular injury marker levels (ULDH) in group IV were significantly lower than in group III and lidocaine also reduced lipid peroxidation during reperfusion. This is in agreement with the histological results. The results of the present study can be taken as evidence of the cytoprotective effect of lidocaine, which may therefore be accepted as a useful agent for kidney preservation.
 


Key words
Cold-storage · Lidocaine · Kidney · Isolated kidney perfusion


Reprint requests
Dr. Serdar Erkasap, Hasan Polatkan Bulvari, Akin Sitesi, No: 122/13, Eskisehir, Turkey, fax : +90 222 2393772, e-mail: serkasap@ogu.edu.tr
 


PHYSIOLOGICAL RESEARCH
© 2002 by the Institute of Physiology, Czech Academy of Sciences

ISSN 0862 - 8408

Issue 5