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Advanced Glycation End-Products and Advanced
Oxidation Protein Products in Patients with Diabetes Mellitus
M. KALOUSOVÁ1, 3, J. ŠKRHA2,
T. ZIMA3
1First Institute of Medical Chemistry and
Biochemistry, 2Third Department of Medicine and
3Institute of Clinical Chemistry, First Faculty of
Medicine, Charles University, Prague, Czech Republic
Received October 22, 2001
Accepted April 2, 2002
Summary
Accelerated glycoxidation takes part in the development of
diabetic complications. We determined advanced glycation
end-products (AGEs) and advanced oxidation protein products
(AOPP) in the sera of 52 patients with diabetes mellitus (DM) -
18 with DM Type 1 and 34 with DM Type 2 and examined their
relationship to the compensation of the disease. AGEs were
estimated spectrofluorimetrically (350 nm/440 nm) whereas AOPP
were determined spectro-photometrically (340 nm). AGEs were
elevated only in DM Type 2 (DM2 5.11±1.15x103 AU/g vs controls
4.08±0.71x103 AU/g, p<0.001, vs DM1 4.14±0.86x103 AU/g, p<0.005,
DM1 vs controls were not significant). AOPP were elevated
significantly in both types of DM with higher levels in DM Type
2 (DM2 157.50±75.15 mmol/l vs healthy subjects 79.80±23.72
mmol/l, p<0.001, vs DM1 97.50±30.91 mmol/l, p<0.005, DM1 vs
controls p<0.05). There was a tight correlation between AGEs and
AOPP in both types of DM (DM1 r=0.75, DM2 r=0.47 (p<0.05)) and
both AGEs and AOPP correlated with triglycerides. In DM Type 1
only, AGEs correlated with HbA1c r=0.47 (p<0.05) and with blood
glucose. Slight but not significant differences in AGEs and AOPP
levels were observed in patients with or without diabetic
complications. Oxidative stress is increased in both types of
DM, more in Type 2 where it contributes to the formation of
glycoxidation products.
Key
words
Advanced glycation end-products · Advanced oxidation protein
products · Diabetes mellitus · Oxidative stress
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Marta Kalousová, M.D., First Institute of Medical Chemistry and
Biochemistry, First Faculty of Medicine, Charles University,
Kateřinská 32, 121 08 Prague 2, Czech Republic. E-mail:
mkalousova@hotmail.com
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