Physiol. Res. 51: 597-604, 2002


Advanced Glycation End-Products and Advanced Oxidation Protein Products in Patients with Diabetes Mellitus


1First Institute of Medical Chemistry and Biochemistry, 2Third Department of Medicine and 3Institute of Clinical Chemistry, First Faculty of Medicine, Charles University, Prague, Czech Republic

Received October 22, 2001
Accepted April 2, 2002

Accelerated glycoxidation takes part in the development of diabetic complications. We determined advanced glycation end-products (AGEs) and advanced oxidation protein products (AOPP) in the sera of 52 patients with diabetes mellitus (DM) - 18 with DM Type 1 and 34 with DM Type 2 and examined their relationship to the compensation of the disease. AGEs were estimated spectrofluorimetrically (350 nm/440 nm) whereas AOPP were determined spectro-photometrically (340 nm). AGEs were elevated only in DM Type 2 (DM2 5.11±1.15x103 AU/g vs controls 4.08±0.71x103 AU/g, p<0.001, vs DM1 4.14±0.86x103 AU/g, p<0.005, DM1 vs controls were not significant). AOPP were elevated significantly in both types of DM with higher levels in DM Type 2 (DM2 157.50±75.15 mmol/l vs healthy subjects 79.80±23.72 mmol/l, p<0.001, vs DM1 97.50±30.91 mmol/l, p<0.005, DM1 vs controls p<0.05). There was a tight correlation between AGEs and AOPP in both types of DM (DM1 r=0.75, DM2 r=0.47 (p<0.05)) and both AGEs and AOPP correlated with triglycerides. In DM Type 1 only, AGEs correlated with HbA1c r=0.47 (p<0.05) and with blood glucose. Slight but not significant differences in AGEs and AOPP levels were observed in patients with or without diabetic complications. Oxidative stress is increased in both types of DM, more in Type 2 where it contributes to the formation of glycoxidation products.

Key words
Advanced glycation end-products · Advanced oxidation protein products · Diabetes mellitus · Oxidative stress

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Marta Kalousová, M.D., First Institute of Medical Chemistry and Biochemistry, First Faculty of Medicine, Charles University, Kateřinská 32, 121 08 Prague 2, Czech Republic. E-mail:

© 2002 by the Institute of Physiology, Czech Academy of Sciences

ISSN 0862 - 8408

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