Physiol. Res. 52: 73-78, 2003

Hepatotoxic Effect of D-Galactosamine and Protective Role of Lipid Emulsion


Department of Physiology, Medical Faculty, Charles University, Hradec Králové and 1Institute of Physiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic

Received February 6, 2002
Accepted May 27, 2002

D-galactosamine is a hepatotoxic agent, which induces diffuse injury of liver tissue followed by the regeneration process. Our data showed a high increase of serum aminotransferases after D-galactosamine administration, which indicates a high extent of liver injury. When lipid emulsion was applied immediately after D-galactosamine, the increase of serum aminotransferases was greatly reduced. In addition, the decrease of the cytochrome c oxidase activity induced by D-galactosamine was not observed after lipid emulsion administration and the increase of total liver oxidative capacity in the regeneration period due to activated mitochondrial biogenesis was accelerated. All these findings indicate a protective effect of lipid emulsion administration against D-galactosamine toxicity.

Key words
D-galactosamine hepatotoxicity • Liver regeneration • Cytochrome c oxidase • Lipid emulsion

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Doc.MUDr. Zuzana Červinková, CSc., Department of Physiology, Medical Faculty, Charles University in Hradec Králové, Šimkova 870, 500 01 Hradec Králové, Czech Republic, e-mail:

© 2003 by the Institute of Physiology, Czech Academy of Sciences