Physiol. Res. 52: 367-372, 2003

Quaternary Benzo[c]phenanthridine Alkaloids as Inhibitors of Dipeptidyl Peptidase IV-Like Activity Bearing Enzymes in Human Blood Plasma and Glioma Cell Lines


1Laboratory of Cancer Cell Biology, Second Institute of Medical Chemistry and Biochemistry, Charles University, Prague, Czech Republic, 2Institute of Medical Chemistry and Biochemistry, Palacký University, Olomouc, Czech Republic

Received  May 13, 2002
Accepted August 12, 2002

Quaternary benzo[c]phenanthridine alkaloids (QBA), fagaronine (FA), sanguinarine (SA), chelerythrine (CHE) and the QBA extract from Macleya cordata (EX) exerted differential inhibitory effect on the hydrolytic activity of particular dipeptidyl peptidase (DPP)-like enzyme isolated from human blood plasma and from human and rat glioma cell lines. The low-MW form of DPP-IV-like enzyme activity, corresponding most probably to DPP-8, observed only in glioma cells but not in human plasma, was inhibited preferentially by SA, CHE and EX, and only slightly by FA. The alkaloid inhibitory effect was concentration-dependent in the range 25-150 M and directly pH-related. In addition, a subtle but consistent inhibition of the intermediate-MW form of DPP-IV-like enzyme activity, ascribed to DPP-IV/CD26, observed only in human plasma and of the attractin (high-MW form of DPP-IV-like enzyme activity, expressed in U87 glioma cells) by the studied alkaloids was observed. We conclude that some of the QBA biological effects could be determined by tissue and cell type specific dipeptidyl peptidase IV-like molecules expression pattern.

Key words
Fagaronine • Sanguinarine • Chelerythrine • Macleya cordata • Dipeptidyl peptidase IV • Attracti

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© 2003 by the Institute of Physiology, Czech Academy of Sciences