Physiol. Res. 52: 291-297, 2003

Office Blood Pressure, Heart Rate and A(-596)G Interleukin-6 Gene Polymorphism in Apparently Healthy Czech Middle-Aged Population


Institute of Pathological Physiology, Faculty of Medicine, Masaryk University and 1First Clinic of Internal Medicine, St. Ann´s Faculty Hospital, Masaryk University, Brno, Czech Republic

Received February 2, 2002
Accepted June 24, 2002

The aim of the study was to assess the association between promoter polymorphism [A(-596)G] in interleukin-6 gene and office systolic and diastolic blood pressures, and the heart rate (HR) in apparently healthy Czech subjects. Furthermore, we evaluated the possible influence of gender, BMI and smoking on these supposed associations. An age-matched (40-50 years) and gender-matched (F/M=81/89) sample of apparently healthy Czech subjects (n=170, F/M=81/89) without hypertension, other cardiovascular diseases or diabetes was examined. The A(-596)G Il-6 gene polymorphism was detected by the PCR method. No differences in genotype distribution and/or allelic frequency was found between groups with lower systolic blood pressure (£ 122 mm Hg) and higher systolic blood pressure (> 122 mm Hg). Similarly, no differences in the IL-6 polymorphism were found between lower (£ 86 mm Hg) and higher (> 86 mm Hg) diastolic blood pressure groups. However, we proved a significant increase of genotypes AG+GG as well as the allele (‑596)G in higher (>78 beats/min) heart rate group. The genotypes AG+GG represent significantly higher relative risk for higher HR frequency, especially in women. Among lean persons with a low heart rate frequency, fewer AG+GG genotypes were determined than among any other subjects. The genotypes AG+GG are more frequent in non-smoking persons with higher HR compared to non-smoking subjects with lower HR, especially in women. Gender, BMI and smoking substantially modify the distribution of A(-596)G Il-6 gene polymorphism in apparently healthy persons with lower or higher heart rate.

Key words
Blood pressure • Heart rate • Interleukin 6 • Gene polymorphism • Healthy subjects • BMI • Smoking

Reprint requests
Anna Vašků, M.D., PhD., Institute of Pathological Physiology, Faculty of Medicine, Masaryk University, Komenského nám. 2, 662 43 Brno, Czech Republic, e-mail:

© 2003 by the Institute of Physiology, Czech Academy of Sciences