Time Course of Leukocyte Response and Free Radical Release
in an Early Reperfusion Injury of the Superior Mesenteric Artery
J. HAMAR1, I.
RÁCZ1, M. ČÍŽ2, A. LOJEK2,
É. PÁLLINGER3, J. FŰRÉSZ3
1National Institute of Traumatology,
Budapest, Hungary, 2Institute of Biophysics,
Academy of Sciences of the Czech Republic, Brno, Czech
Republic and 3Central Army Research Institute,
Budapest, Hungary
Received May 2, 2002
Accepted August 1, 2002
Summary
The sequence of changes in circulating immune cells and in free
radical production was studied during the small intestine
reperfusion. Rat small intestine ischemia/reperfusion was
induced by a 45 min superior mesenteric artery occlusion
followed by a 4-hour reperfusion. Samples of peripheral blood
were collected every 20 min during reperfusion. While the number
of polymorphonuclear leukocytes increased significantly both in
the sham-operated controls and the experimental group (about 400
% at the end of reperfusion), a decrease in lymphocyte counts to
60 % was observed in the experimental group only. Although there
were no changes in the counts of total T lymphocytes, a
significant reduction in B cell counts was observed. Flow-cytometrical
measurements showed no changes in the Tc subpopulation, while
the Th subpopulation increased in the experimental group only.
Free radical generation in blood (luminometric measurements)
increased gradually and reached an eight-fold level by the end
of reperfusion in both groups. Thus, it has been shown that the
increase in free radical production is mainly due to the
increased number of polymorphonuclear leukocytes mobilized
already at the initial stages of reperfusion. The reduction in B
lymphocyte population is probably due to homing mechanisms.
Key
words
Rat • Polymorphonuclear leukocytes • Lymphocytes •
Chemiluminescence
Reprint
requests
János Hamar MD, National
Institute of Traumatology, H-1081 Budapest, Fiumei ut. 17,
Hungary. E-mail:
hamar.otri@freemail.hu
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