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Effect of Resveratrol on Some
Activities of Isolated and in Whole Blood Human Neutrophils
A. CAVALLARO1, T.
AINIS1, C. BOTTARI2, V. FIMIANI
Department of Experimental Pathology and Microbiology,
1Department of Environmental Sanitary Social and Industrial
Protectionistic and 2Department of Surgical Specialities,
Medical School, University of Messina, Messina, Italy
Received July 19, 2002
Accepted November 12, 2002
Summary
Resveratrol, which is a polyphenol present in red wines and
vegetables included in human diets, exerts many biological
effects. The aim of the present study was to investigate its
effect on some activities of polymorphonuclear leukocytes,
particularly the generation of superoxide anion (O2-) in whole
blood, hypochlorous acid (HOCl) and nitric oxide (NO) production
by isolated cells, and chemotaxis. Resveratrol showed
significant dose-dependent inhibitory effect on all these
activities. In particular, it inhibited O2- generation in
stimulated but not in resting neutrophils, decreased HOCl much
more than O2- production indicating an effect on myeloperoxidase
secretion since HOCl production is directly and proportionally
dependent on O2- generation and reduced cell motility. The small
dose of resveratrol (4.38 nM) used is attainable with a diet
including red wine and vegetables confirming its protective role
against some pathological processes such as inflammation,
coronary heart disease, and cancer.
Key words
Resveratrol • Neutrophils • Reactive oxygen species • Chemotaxis
Reprint requests
Vincenzo Fimiani, Department of Experimental Pathology and
Microbiology, Pathology General Section, Policlinico
Universitario, Torre Biologica, Via Consolare Valeria, I-98125
Messina, Italy, e-mail :
Vincenzo.Fimiani@unime.it
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