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Dynamics of Cytokine
Production in Human Peripheral Blood Mononuclear Cells
Stimulated by LPS or Infected by Borrelia
L. JANSKÝ1, P. REYMANOVÁ1,
J. KOPECKÝ2
1Faculty of Biology, University of South Bohemia and
2Institute of Parasitology, Academy of Sciences of
the Czech Republic, České Budějovice, Czech Republic
Received August 20, 2002
Accepted May 7, 2003
Summary
To specify the role
of individual cytokines in the immune response to pyrogens,
isolated and cultivated human peripheral blood mononuclear cells
(PBMC) were used for the experiments. Different pyrogens
(lipopolysaccharide from Escherichia coli - LPS and live
Borrelia afzelii) were applied and the time course of
changes in concentrations of different cytokines in the medium
was followed using the ELISA method. It was found that
nonstimulated human PBMC proliferate under in vitro
conditions and produce IL-6, TNF-a,
IL-10 and finally also IL-1b.
Productions of IL-12 and INF-g
are not changed. Proliferation of PBMC is potentiated after
incubation with LPS or live Borrelia. PBMC stimulated by
LPS increase the net production (stimulated minus unstimulated)
of IL-1b
and TNF-a
significantly, while production of IL-6 was smaller. A delayed
increase in the production of IL-10 was also observed.
Productions of IL-12 and INF-g
were not influenced. In contrast to LPS, stimulation of PBMC
with live Borrelia, increases also the production of
IL-12 and IFN-g,
besides IL-1b,
TNF-a,
IL-6 and IL-10. Productions of IL-1b,
IL-6 and TNFa
increased immediately after incubation with both LPS and
Borrelia, while productions of IL-12 and INF-g
begin to increase 8 hours and production of IL-10 12 hours after
stimulation. Data indicate that stimulation with different
pyrogens may activate the cells of the immune cascade in a
different way. Stimulation of BPMC by LPS seems to activate the
initial steps of the immune response (macrophages and
granulocytes) only, while infection with live Borrelia
also stimulates the later phase of the immune response, probably
due to effect of initially produced cytokines.
Key words
Fever • Cytokines • Lipopolysaccharide • Borrelia
Reprint requests
F. Vyskočil, Institute of Physiology, Academy of Sciences of the
Czech Republic, Vídeňská 1083, 142 20 Prague 4, Czech Republic,
e-mail:
vyskocil@biomed.cas.cz
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