Altered Blood-Brain Barrier
Permeability and Its Effect on the Distribution of Evans Blue
and Sodium Fluorescein in the Rat Brain Applied by Intracarotid
Injection
P. KOZLER, J. POKORNÝ1
Department of Neurosurgery, Central Military Hospital, Prague
and 1Institute of Physiology, First Faculty of
Medicine, Charles University, Prague, Czech Republic
Received February 25, 2002
Accepted October 30, 2002
Summary
The aim was to study the blood-brain permeability according to
the distribution in the rat brain of Evans blue (EB) and sodium
fluorescein (NaFl) administered by an intracarotid injection.
Eighteen animals were divided into six groups according to the
state of the blood-brain barrier (BBB) at the moment when the
dyes were being applied. In the first two groups, the BBB was
intact, in groups 3 and 4 the barrier had been opened
osmotically prior to the application of the dyes, and in groups
5 and 6 a cellular edema was induced by hyperhydration before
administration of the dyes. The intracellular and extracellular
distribution of the dyes was studied by fluorescence microscopy.
The histological picture thus represented the morphological
correlate of the way BBB permeability had been changed before
the application of the dyes.
Key words
Blood-brain barrier • Cellular edema • Intravital dyes •
Fluorescence microscopy
Reprint requests
MUDr Petr Kozler, Neurosurgical Clinic, Central Military
Hospital, U vojenské nemocnice 1200, 169 02 Prague 6, Czech
Republic. e-mail: nchr@uvn.cz
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