Physiol. Res. 53: 97-102, 2004

Effect of Stobadine on Opsonized Zymosan Stimulated Generation of Reactive Oxygen Species in Human Blood Cells


Institute of Experimental Pharmacology Slovak Academy of Sciences, Bratislava, Slovak Republic,
1Biophysical Institute, Czech Academy of Sciences, Brno, Czech Republic

Received November 19, 2002
Accepted March 20, 2003

To predict more precisely the effect of stobadine, a pyridoindole antioxidant agent, in the whole organism, we studied its effect on opsonized zymosan-stimulated free radical generation in whole blood, on superoxide generation in the mixture of PMNL : platelets (1:50), as well as on superoxide generation and myeloperoxidase release in isolated PMNL. Without stimulation, stobadine had no effect on reactive oxygen species (ROS) generation and myeloperoxidase release. Stobadine in a concentration of 10 or 100 µmol/l significantly decreased luminol-enhanced chemiluminescence in opsonized zymosan-stimulated whole blood. In concentrations of 10 and 100 µmol/l, it reduced myeloperoxidase release from isolated neutrophils. Stobadine significantly decreased superoxide generation in isolated neutrophils in 100 µmol/l concentration. Its effect was much less pronounced in the mixture of neutrophils and platelets in the ratio close to physiological conditions (1:50). Our results suggest that stobadine might exert a beneficial effect in diseases or states where superfluous ROS generation could be deleterious.

Key words
Human blood cells • Opsonized zymosan • Stobadine • Reactive oxygen species

© 2004 by the Institute of Physiology, Czech Academy of Sciences