IL-10 Does Not Affect Oxidative Burst and
Expression of Selected Surface Antigen on Human Blood Phagocytes in vitro
L. GALLOVÁ1,2, L. KUBALA1, M. ČÍŽ1, A.
LOJEK1
1Institute of Biophysics, Academy of Sciences of the Czech
Republic and 2Department of Comparative Animal Physiology and
General Zoology, Faculty of Science, Masaryk University, Brno, Czech
Republic
Received February 25, 2003
Accepted April 15 2003
Summary
Cytokines play a major role in the control of inflammatory responses,
participate in the regulation of blood phagocyte activities and as such
are used for immunomodulatory therapy. In the present study, the influence
of IL-10 on human blood phagocyte activity in the presence/absence of
IL-6, IL-8 and TNF-a was tested
in vitro. Our research analyzed the effects of cytokines on the production
of reactive oxygen species measured by chemiluminescence and flow
cytometry, and on the expression of surface molecules (CD11b, CD15, CD62L,
CD31) measured by flow cytometry. IL-10 had no inhibitory effect on
reactive oxygen species production and the expression of any examined
adhesion molecule by resting or stimulated blood phagocytes within 3 h of
incubation. Conversely, TNF-, IL-6, and IL-8 increased reactive oxygen
species production and the expression of CD11b and CD15 on both
neutrophils and monocytes and decreased the expression of CD62L. These
priming effects of the tested pro-inflammatory cytokines were not affected
by IL-10. The obtained results suggest that IL-10 does not directly
control blood phagocyte activation. These results also provide better
information about the contribution of IL-6, IL-8 and TNF- to the
regulation of blood phagocyte-mediated inflammatory processes.
Key words
Interleukins • TNF-a • Leukocytes
• Oxidative burst • Surface molecules
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