Physiol. Res. 53: 199-208, 2004

IL-10 Does Not Affect Oxidative Burst and Expression of Selected Surface Antigen on Human Blood Phagocytes in vitro


1Institute of Biophysics, Academy of Sciences of the Czech Republic and 2Department of Comparative Animal Physiology and General Zoology, Faculty of Science, Masaryk University, Brno, Czech Republic

Received February 25, 2003
Accepted April 15 2003

Cytokines play a major role in the control of inflammatory responses, participate in the regulation of blood phagocyte activities and as such are used for immunomodulatory therapy. In the present study, the influence of IL-10 on human blood phagocyte activity in the presence/absence of IL-6, IL-8 and TNF-a was tested in vitro. Our research analyzed the effects of cytokines on the production of reactive oxygen species measured by chemiluminescence and flow cytometry, and on the expression of surface molecules (CD11b, CD15, CD62L, CD31) measured by flow cytometry. IL-10 had no inhibitory effect on reactive oxygen species production and the expression of any examined adhesion molecule by resting or stimulated blood phagocytes within 3 h of incubation. Conversely, TNF-, IL-6, and IL-8 increased reactive oxygen species production and the expression of CD11b and CD15 on both neutrophils and monocytes and decreased the expression of CD62L. These priming effects of the tested pro-inflammatory cytokines were not affected by IL-10. The obtained results suggest that IL-10 does not directly control blood phagocyte activation. These results also provide better information about the contribution of IL-6, IL-8 and TNF- to the regulation of blood phagocyte-mediated inflammatory processes.

Key words
Interleukins • TNF-a • Leukocytes • Oxidative burst • Surface molecules

© 2004 by the Institute of Physiology, Czech Academy of Sciences