Does Cd36 Gene Play a Key Role in
Disturbed Glucose and Fatty Acid Metabolism in Prague Hypertensive
Hypertriglyceridemic Rats?
M. KADLECOVÁ, J. ČEJKA, J. ZICHA, J. KUNEŠ
Institute of Physiology, Academy of Sciences of the Czech Republic and
Center for Experimental Research of Cardiovascular Diseases, Prague, Czech
Republic
Received September 30, 2003
Accepted December 20, 2003
Summary
Close links between hypertension, hypertriglyceridemia, insulin resistance
and other symptoms of metabolic syndrome was demonstrated in humans and
experimental animals. Quantitative trait loci for defects in glucose and
fatty acid metabolism, hypertriglyceridemia and hypertension were mapped
in spontaneously hypertensive rats (SHR) on chromosome 4 and defective
Cd36 gene was identified in this region. Here we investigated the
polymorphism of Cd36 gene in Prague hereditary hypertriglyceridemic
(HTG) rats, which represent another model of genetic hypertension and
metabolic syndrome. These animals were compared with NIH-derived SHR and
two different normotensive control strains (WKY, LEW). In spite of the
fact that HTG and SHR rats had similar metabolic disturbances, genotype
analysis of PCR products has shown that Cd36 mutation was not
present in HTG rats. In conclusion, we have revealed that defective
Cd36 is probably a candidate gene for disorded fatty-acid metabolism,
glucose intolerance and insulin resistance in NIH-derived SHR, but other
genes might play a role in pathogenesis of metabolic syndrome in Prague
hereditary hypertriglyceridemic rats. This is in accordance with the
absence of defective Cd36 gene in original SHR from Japan.
Key words
Hypertension • Insulin resistance • Fatty acid • Oral glucose test •
Metabolic syndrome
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