Wine Polyphenols Stimulate
Superoxide Anion Production to Promote Calcium Signaling and
Endothelial-Dependent Vasodilatation
J. DUARTE1, E. ANDRIAMBELOSON, M. DIEBOLT, R.
ANDRIANTSITOHAINA
Laboratoire de Pharmacologie et Physico-Chimie des Interactions
Cellulaires et Moléculaires, Université Louis Pasteur de
Strasbourg, CNRS, Faculté de Pharmacie, Illkirch, France and
1Department of Pharmacology, Faculty of Pharmacy,
University of Granada, Granada, Spain
Received December 4, 2003
Accepted April 29, 2004
Summary
The present study was aimed to evaluate the mechanisms involved
in the vasorelaxant effects of red wine polyphenol compounds
(RWPC) in small mesenteric rat arteries. RWPC produce relaxation
in small mesenteric arteries. This relaxant effect was abolished
by endothelial denudation, NO-synthase blockade with L-NAME and
partial depolarization with KCl or L-NAME plus KCl. Incubation
with the reactive oxygen species scavenger, superoxide dismutase
(SOD) plus catalase, or inhibition of NAD(P)H-dependent
oxidoreductases with diphenyleneiodonium also inhibited RWPC
induced vascular relaxation. Application of RWPC elicited a
transient increase in intracellular calcium concentration
([Ca2+]i) in bovine aortic endothelial cells (BAEC), which was
attenuated by a mixture of SOD and catalase. Incubation of BAEC
with RWPC increased the SOD inhibitable production of O2-. These
results suggest the involvement of O2- in the [Ca2+]i increase
evoked by RWPC, leading to the activation of enzymes involved in
the release of endothelial relaxant factors and subsequent
vasodilatation of resistance arteries.
Key words
Red wine polyphenol compounds • Nitric oxide • Calcium •
Superoxide anions • Endothelium • Mesenteric artery
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