The Effects of Parenteral
Lipid Emulsions on Cancer and Normal Human Colon Epithelial
Cells in vitro
J. HOFMANOVÁ1, Z. ZADÁK2,
R. HYŠPLER2, J. MIKESKA3, P. ŽĎÁNSKÝ2,
A. VACULOVÁ1, J. NETÍKOVÁ1, A. KOZUBÍK1
1Laboratory of Cytokinetics, Institute of Biophysics,
Academy of Sciences of the Czech Republic, Brno, 2Department
of Metabolic Care and Gerontology, Charles University, Teaching
Hospital, Hradec Králové and 3Infusia Hořátev, a. s.,
Czech Republic
Received June 14, 2004
Accepted October 19, 2004
On-line available December 9, 2004
Summary
Differences in lipid metabolism of tumor and normal tissues
suggest a distinct response to available lipid compounds. In
this study, the in vitro effects of five types of commercial
parenteral lipid emulsions were investigated on human cell lines
derived from normal fetal colon (FHC) or colon adenocarcinoma
(HT-29). Changes of the cellular lipid fatty acid content, cell
oxidative response, and the cell growth and death rates were
evaluated after 48 h. No effects of any type of emulsions were
detected on cell proliferation and viability. Compared to the
controls, supplementation with lipid emulsions resulted in a
multiple increase of linoleic and linolenic acids in total cell
lipids, but the content of arachidonic, eicosapentaenoic, and
docosahexaenoic acids decreased particularly in HT-29 cells. The
concentration of emulsions which did not affected HT-29 cells
increased the percentage of floating and subG0/G1 FHC cells
probably due to their higher reactive oxygen species production
and lipid peroxidation. Co-treatment of cells with antioxidant
Trolox reduced the observed effects. Our results imply that
lipid emulsions can differently affect the response of colon
cells of distinct origin.
Key words
Fat emulsion • Reactive oxygen • Lipid peroxidation • Apoptosis
• Tumor cells
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