Physiol. Res. 54: 585-591, 2005

Biochemical Changes of Enzymatic Defense System after Phenanthrene Exposure in Olive Flounder, Paralichthys olivaceus

J.-H. JEE1, J.-C. KANG2

1Institute of Fisheries Sciences and 2Department of Aquatic Life Medicine, Pukyong National University, Busan, Korea

Received August 17, 2004
Accepted October 21, 2004
On-line available February 16, 2005

The objective of this study was to investigate the early biological response in the olive flounder exposed to sub-lethal concentrations of waterborne phenanthrene (0.5, 1 or 2 μM). The fish were exposed for 4 weeks and we analyzed their enzymatic defense system, antioxidant and phase II enzyme activities, to evaluate the chronic exposure toxicity of phenanthrene. Waterborne phenanthrene affected antioxidant enzymes and glutathione-mediated detoxification as an enzyme defense system. Hepatic, gill and kidney glutathione reductase as well as glutathione S-transferase, and catalase activities were markedly elevated after two or four weeks of exposure. These enzyme activities of the olive flounder, Paralichthys olivaceus, seem to be a convenient approach for monitoring pollution in coastal areas against polycyclic aromatic hydrocarbon pollution including phenanthrene.

Key words
Antioxidant enzymes Detoxification Olive flounder Phase II enzyme Phenanthrene

2005 by the Institute of Physiology, Czech Academy of Sciences