Physiol. Res. 55: 151-156, 2006


The Effects of Short-Term Training on Platelet Functions and Total Antioxidant Capacity in Rats

H. FICICILAR1, A.M. ZERGEROGLU2, G. ERSOZ1, A. ERDOGAN2,
S. OZDEMIR3, D. TEKIN1


Ankara University, School of Medicine, Departments of 1Physiology, 2Sports Medicine and 3Biophysics, Ankara, Turkey

Received January 28, 2005
Accepted May 3, 2005
On-line available May 24, 2005


Summary
The purpose of the present study was to investigate the effect of short-term endurance training on plasma total antioxidant status (TAS) and on in vitro platelet aggregation and ATP release. Blood samples were collected from the abdominal aorta of rats following short-term treadmill exercise (25 m/min, 0 % grade, 30 min) for three consecutive days, as well as in non-exercised control group. Platelet aggregation and platelet ATP release were evaluated by impedance and bioluminescence techniques, respectively. Plasma TAS was measured spectrophotometrically. Plasma TAS was higher and ADP-induced platelet ATP release was lower in the short-term training group with respect to the control group (p<0.001). Significant negative correlation (r = -0.56, p<0.05) was found between plasma TAS and ADP-induced platelet ATP release. Neither ADP- and collagen-induced maximum aggregation rate nor collagen-induced platelet ATP release were significantly different between the groups. According to these results, short-term training caused an alteration in platelet functions limited to the secretion response, which may be related to the oxidant/antioxidant balance changes favoring the antioxidants. The improved plasma total antioxidant capacity was possibly sufficient to prevent exercise-induced oxidative damage, and the adaptive response of platelets might be associated with enhanced antioxidant status.


Key words
Exercise Antioxidant capacity Platelet Aggregation Secretion
 


2006 by the Institute of Physiology, Czech Academy of Sciences