The Effects of Short-Term
Training on Platelet Functions and Total Antioxidant Capacity in
Rats
H. FICICILAR1, A.M. ZERGEROGLU2,
G. ERSOZ1, A. ERDOGAN2,
S. OZDEMIR3, D. TEKIN1
Ankara University, School of Medicine, Departments of 1Physiology,
2Sports Medicine and 3Biophysics, Ankara, Turkey
Received January 28, 2005
Accepted May 3, 2005
On-line available May 24, 2005
Summary
The purpose of the present study was to investigate the effect
of short-term endurance training on plasma total antioxidant
status (TAS) and on in vitro platelet aggregation and ATP
release. Blood samples were collected from the abdominal aorta
of rats following short-term treadmill exercise (25 m/min, 0 %
grade, 30 min) for three consecutive days, as well as in
non-exercised control group. Platelet aggregation and platelet
ATP release were evaluated by impedance and bioluminescence
techniques, respectively. Plasma TAS was measured
spectrophotometrically. Plasma TAS was higher and ADP-induced
platelet ATP release was lower in the short-term training group
with respect to the control group (p<0.001). Significant
negative correlation (r = -0.56, p<0.05) was found between
plasma TAS and ADP-induced platelet ATP release. Neither ADP-
and collagen-induced maximum aggregation rate nor
collagen-induced platelet ATP release were significantly
different between the groups. According to these results,
short-term training caused an alteration in platelet functions
limited to the secretion response, which may be related to the
oxidant/antioxidant balance changes favoring the antioxidants.
The improved plasma total antioxidant capacity was possibly
sufficient to prevent exercise-induced oxidative damage, and the
adaptive response of platelets might be associated with enhanced
antioxidant status.
Key words
Exercise • Antioxidant capacity • Platelet • Aggregation •
Secretion
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