Physiol. Res. 55: 189-194, 2006

The Response of Na+/K+-ATPase of Human Erythrocytes to Green Laser Light Treatment


Division of Biomedical Physics, Faculty of Mathematics, Physics, and Computer Science, Comenius University, Bratislava, Slovak Republic and 1Department of General Biophysics, University of Lodz, Łódź, Poland

Received September 30, 2004
Accepted April 4, 2005
On-line available May 24, 2005

The objective of this study was to investigate the response of Na+/K+-ATPase of human erythrocytes to green laser irradiation. Effects of green laser light of fluences 9.5-63.3 and merocyanine 540-mediated laser light treatment were studied. Isolated erythrocyte membranes (protein concentration of 1 mg/ml) were irradiated by Nd:YAG laser (532 nm, 30 mW) and then incubated in a medium with 2 mM ATP for 30 min. Activity of ATPase was determined colorimetrically by measuring the colored reaction product of liberated inorganic phosphate and malachite green at 640 nm. Contribution of Na+/K+-ATPase to overall phosphate production was determined using ouabain. A positive effect of green laser light on Na+/K+-ATPase activity was observed. The dependence of enzymatically liberated inorganic phosphate on light fluence showed a linear correlation (R2=0.96, P=0.0005) for all fluences applied (9.5-63.3 On the other hand, MC 540-mediated phototreatment caused a suppression of enzyme activity.

Key words
Na+/K+-ATPase activity • Biostimulation • Erythrocyte membrane • Merocyanine 540 • Photodynamic processes

© 2006 by the Institute of Physiology, Czech Academy of Sciences