Cytogenetic and Immunological
Changes after Dermal Exposure to Polycyclic Aromatic
Hydrocarbons and UV Radiation
L. BORSKÁ1, Z. FIALA2, J.
KREJSEK3, K. HAMÁKOVÁ4, C. ANDRÝS3,
J. ŠMEJKALOVÁ2, D. VOKURKOVÁ3, J.
KREMLÁČEK1
1Institute of Pathological Physiology, 2Institute of Hygiene and
Preventive Medicine, Charles University in Prague, Faculty of
Medicine in Hradec Králové, 3Institute of Clinical Immunology
and Allergology, 4Clinic of Dermal and Venereal Diseases,
University Hospital in Hradec Králové, Czech Republic
Received March 23, 2005
Accepted June 30, 2005
On-line available August 5, 2005
Summary
Goeckerman’s therapy (GT), which combines exposure to coal tar
(polycyclic aromatic hydrocarbons – PAHs) and UV radiation (UV)
is often used as the first option for treatment of psoriasis.
However, PAHs and UV represent mutagenic, carcinogenic and
immunotoxic agents. Therefore GT can represent a health risk for
the patients. The group under observation consisted of thirty
patients undergoing GT. Before and after the treatment, blood
samples were collected and chromosomal aberrations and selected
immunological markers were determined. The relationships between
chromosomal aberrations and immunological markers and the extent
(duration) of exposure to GT were evaluated. The Psoriasis Area
and Severity Index (PASI) score confirmed the high efficacy of
GT. However, significantly elevated levels of chromosomal
aberrations of peripheral lymphocytes were also found after the
therapy (p<0.001). The levels of chromosomal abnormalities
correlated to the extent and the total duration of exposure to
PAHs (r = 0.682, p<0.01 and r = 0.605, p<0.05). After the
therapy, significantly decreased levels of IgE, IgM isotypes of
immunoglobulin,
α2-macroglobulin and transferrin together with β2-microglobulin
were found. From the immunological markers listed above only the
decreased level of α2-macroglobulin correlated to the
extent of exposure to PAHs (r = -0.568, p<0.05). No correlation
was found between chromosomal aberrations, significantly changed
immunological markers and the duration of UV exposure. Our study
revealed that GT has a significant impact on both genetic and
immunological parameters of psoriatic patients. The results
indicate that GT could increase genotoxic risk and modulates
immunity of treated patients.
Key words
Psoriasis • UV-radiation • Polycyclic aromatic hydrocarbons •
Genotoxicity • Immunotoxicity
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