Apolipoprotein A5 and
Hypertriglyceridemia in Prague Hypertriglyceridemic Rats
M. KADLECOVÁ, S. HOJNÁ, R. BOHUSLAVOVÁ1, J. A.
HUBÁČEK1, J. ZICHA, J. KUNEŠ
Institute of Physiology AS CR and Cardiovascular Research
Center, 1Institute for Clinical and Experimental Medicine,
Prague, Czech Republic
Received September 5, 2005
Accepted October 3, 2005
On-line October 17, 2005
Summary
High plasma triglyceride (TG) level is a major independent risk
factor of coronary heart disease. A newly identified
Apolipoprotein A5 (Apoa5) gene has been shown to play an
important role in determining plasma TG concentrations in humans
and mice. Prague hereditary hypertriglyceridemic (HTG) rats are
a useful model of human hypertriglyceridemia and other symptoms
of metabolic syndrome. Thus, the variation of Apoa5 gene and its
expression were studied in this strain under normal conditions
and after chronic fructose loading. Lewis and Wistar rats served
as normotriglyceridemic controls. Plasma TG were significantly
higher in HTG rats in comparison with both control strains.
Sequence analysis of the rat Apoa5 gene revealed the existence
of two introns. However, screening of the coding regions and
intron-exon boundaries of Apoa5 gene did not indicate any
mutation of this gene in HTG rats in comparison with Lewis and
Wistar ones. Under the basal conditions the expression of Apoa5
was lower in all age groups of HTG rats compared to Wistar
animals. Furthermore, during chronic fructose loading there were
no significant changes of Apoa5 expression in HTG rats, although
plasma TG levels rose 3-4 times within first two days of
fructose loading and were increased during the whole period of
fructose treatment. In conclusion, Apoa5 does not seem to be a
genetic determinant of hypertriglyceridemia in HTG rats. The
absence of significant changes in Apoa5 gene expression during
chronic fructose-induced TG elevation excludes its major role in
mechanisms compensating severe hypertriglyceridemia.
Key words
Hypertriglyceridemia • Metabolic syndrome • Apolipoprotein A5 •
Genetic analysis • Gene expression • Chronic fructose loading •
Rat
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