Side Specific Effect of
5-Hydroxytryptamine on NaCl Transport in the Apical and
Basolateral Membrane of Rat Tracheal Epithelia
B. D. NAM, H. J. PARK
Department of Pediatrics, College of Medicine, Pusan National
University, Pusan, Korea
Received January 14, 2005
Accepted August 23, 2005
On-line available October 17, 2005
Summary
5-Hydroxytryptamine (5-HT) can be released from mast cells and
platelets through an IgE-dependent mechanism and may play a role
in the pathogenesis of allergic bronchoconstriction. However,
the effect of 5-HT on ion transport by the airway epithelium is
still controversial. The objective of this study was to
determine whether 5-hydroxytryptamine (5-HT) regulates NaCl
transport by different mechanisms in the apical and basolateral
membrane of tracheal epithelia. We studied the rat tracheal
epithelium under short-circuit conditions in vitro.
Short-circuit current (Isc) was measured in rat tracheal
epithelial monolayers cultured on porous filters. 5-HT inhibited
Na+ absorption [measured via Na+
short-circuit current (INasc)] in the apical membrane and
stimulated Cl- secretion [measured via Cl-
short-circuit current (IClsc)] in the basolateral membrane.
Functional localization using selective 5-HT agonists and
antagonists suggest that IClsc is stimulated by the basolateral
membrane-resident 5-HT receptors, whereas INasc is inhibited by
the apical membrane-resident 5-HT2 receptors. The basolateral
addition of 5-HT increases intracellular cAMP content, but its
apical addition does not. The addition of BAPTA/AM blocked the
decrease of INasc which was induced by the apical addition of
5-HT, and 5-HT increased intracellular Ca concentrations. These
results indicate that 5-HT differentially affects INasc and
IClsc across rat tracheal monolayers through interactions with
distinct receptors in the apical and the basolateral membrane.
These effects may result in an increase of water movement
towards the airway lumen.
Key words
5-hydroxytryptamine • Tracheal epithelia • NaCl transport
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