Protective Effect of
S-Adenosylmethionine against Galactosamine-Induced Injury of Rat
Hepatocytes in Primary Culture
O. KUČERA1, Z. ČERVINKOVÁ1,
H. LOTKOVÁ1, P. KŘIVÁKOVÁ1,
T. ROUŠAR1,3, V. MUŽÁKOVÁ3, R. HÉŽOVÁ3,
R. KANĎÁR3, E. RUDOLF2
1Department of Physiology and 2Department
of Medical Biology and Genetics, Faculty of Medicine in Hradec
Králové, Charles University in Prague, Czech Republic, 3Department
of Biological and Biochemical Sciences, Faculty of Chemical
Technology, University of Pardubice, Czech Republic
Received date October 3, 2005
Accepted date December 6, 2005
On-line available December 12, 2005
Summary
The protective effect of S-adenosylmethionine (SAMe) on
D-galactosamine (GalN)-induced damage to rat hepatocytes was
tested in primary cultures. SAMe at concentrations of 50 and
1000 mg/l significantly reduced lactate dehydrogenase release
from cells injured by 40 mM GalN after 24 h of incubation. There
were no significant changes in urea production after 24 h among
tested groups, including control hepatocytes. Exposure of
hepatocytes to GalN leads to 3.5-fold decrease in urea synthesis
after 48 h in comparison with control cell cultures. Addition of
the highest dose of SAMe (1000 mg/l) into the culture media
attenuated this decrease by 180 %. None of the tested doses of
SAMe (5, 25, 50 and 1000 mg/l) affected considerably the reduced
activity of mitochondrial dehydrogenases. The content of reduced
and oxidized glutathione in GalN-exposed cells was diminished to
1.5 % and 16 %, respectively, of the control values after 24 h.
Using only the highest concentration SAMe increased
significantly these contents. SAMe had no effect on dramatically
decreased albumin synthesis. These findings indicate beneficial
effect of SAMe, especially of the highest concentration, on
GalN-induced toxicity to rat hepatocytes in primary culture.
This action of SAMe seems to be associated with reduction of
plasma membrane damage and increased synthesis of glutathione.
Key words
S-adenosylmethionine • Galactosamine • Hepatocytes in vitro
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