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X-ORIGINAL-URL:https://www.biomed.cas.cz
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TZID:Europe/Prague
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DTSTART:20250330T010000
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DTSTART;TZID=Europe/Prague:20250212T150000
DTEND;TZID=Europe/Prague:20250212T160000
DTSTAMP:20260423T232352
CREATED:20250203T151725Z
LAST-MODIFIED:20250203T151725Z
UID:2294-1739372400-1739376000@www.biomed.cas.cz
SUMMARY:Seminář Ludovica Antiga\, M.Sc.
DESCRIPTION:“PIP2-RNA-protein interactions in phase separation of Pol II transcription” \nThe RNA Polymerase II transcription is a multifaceted process crucial for gene expression in eukaryotic cells. Over the last decade\, liquid-liquid phase separation (LLPS) was shown to be crucial in regulation of RNA pol II transcription. We have shown that nuclear phosphoinositides are pivotal for regulating biological processes and PIP2 has been shown to localize with RNA Pol II machinery and nascent RNA\, linking it to the initiation step of transcription. Here\, by using lipid-interacting RNA sequencing (LIPRNAseq) and confocal microscopy\, we found a PIP2-binding AU-rich RNA motif and identified the long non-coding RNA HANR (lncHANR) in complex with PIP2 as a part of oncogenic super-enhancers in perinucleolar region of the nucleus.Furthermore\, we used PIP2-pull down coupled with mass spectrometry to identify RNA-dependent PIP2 interactome associated with nuclear processes emphasizing the role of PIP2 in liquid-liquid phase separation of RNA Pol II transcription machinery. \n 
URL:https://www.biomed.cas.cz/event/seminar-ludovica-antiga-m-sc/
LOCATION:Posluchárna Milana Haška / Milan Hašek Auditorium
ORGANIZER;CN="%C3%9AMG":MAILTO:leona.krausova@img.cas.cz
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