BEGIN:VCALENDAR VERSION:2.0 PRODID:-// - ECPv6.6.3//NONSGML v1.0//EN CALSCALE:GREGORIAN METHOD:PUBLISH X-ORIGINAL-URL:https://www.biomed.cas.cz X-WR-CALDESC:Akce na REFRESH-INTERVAL;VALUE=DURATION:PT1H X-Robots-Tag:noindex X-PUBLISHED-TTL:PT1H BEGIN:VTIMEZONE TZID:Europe/Prague BEGIN:DAYLIGHT TZOFFSETFROM:+0100 TZOFFSETTO:+0200 TZNAME:CEST DTSTART:20250330T010000 END:DAYLIGHT BEGIN:STANDARD TZOFFSETFROM:+0200 TZOFFSETTO:+0100 TZNAME:CET DTSTART:20251026T010000 END:STANDARD BEGIN:DAYLIGHT TZOFFSETFROM:+0100 TZOFFSETTO:+0200 TZNAME:CEST DTSTART:20260329T010000 END:DAYLIGHT BEGIN:STANDARD TZOFFSETFROM:+0200 TZOFFSETTO:+0100 TZNAME:CET DTSTART:20261025T010000 END:STANDARD END:VTIMEZONE BEGIN:VEVENT DTSTART;TZID=Europe/Prague:20260415T150000 DTEND;TZID=Europe/Prague:20260415T160000 DTSTAMP:20260418T213029 CREATED:20260408T101152Z LAST-MODIFIED:20260408T101152Z UID:2516-1776265200-1776268800@www.biomed.cas.cz SUMMARY:Seminář Despoina Giamaki DESCRIPTION:“A hidden layer of chromatin regulation in neurological disease” \nGenome stability and DNA repair are essential for human health. ADP-ribosylation is a key post-translational modification in the DNA damage response\, regulated by PARP enzymes. Our research focuses on ARH3\, an enzyme that removes mono-ADP-ribose from proteins\, as its deficiency is linked to rare neurological disease. Using patient-derived cells\, 3D brain organoids and mouse models\, we show that loss of ARH3 leads to accumulation of mono-ADP-ribose on chromatin. Genome-wide analyses (CUT&Tag\, spatial transcriptomic and RNA-seq) reveal that these changes alter gene expression and modulate other histone modifications. We further explore how these chromatin alterations affect responses to stress\, including viral infections\, potentially contributing to neuropathology. Our findings provide new insights into disease mechanisms and highlight ADP-ribosylation as a potential therapeutic target in neurological disorders. URL:https://www.biomed.cas.cz/event/seminar-despoina-giamaki/ LOCATION:Posluchárna Milana Haška / Milan Hašek Auditorium ORGANIZER;CN="%C3%9AMG":MAILTO:leona.krausova@img.cas.cz END:VEVENT BEGIN:VEVENT DTSTART;TZID=Europe/Prague:20260408T150000 DTEND;TZID=Europe/Prague:20260408T160000 DTSTAMP:20260418T213029 CREATED:20260331T120116Z LAST-MODIFIED:20260408T101210Z UID:2512-1775660400-1775664000@www.biomed.cas.cz SUMMARY:Seminář Lumír Krejčí DESCRIPTION:“Homologous Recombination – From repair of DSBs to processing stalled replication forks and human diseases” \nHomologous recombination (HR) is a central genome maintenance pathway that safeguards DNA integrity during both double-strand break (DSB) repair and replication stress. A key intermediate of HR is the nucleoprotein filament formed by RAD51 on single-stranded DNA\, which mediates homology search and strand invasion. During DSB repair RAD51 filament assembly is tightly regulated by mediator proteins\, including BRCA2\, and RAD51 paralogs\, ensuring accurate repair using a homologous template and preventing genome instability. Beyond canonical DSB repair\, HR plays a crucial role at stalled replication forks\, where RAD51 stabilises nascent DNA\, protects forks from nucleolytic degradation\, and promotes fork reversal and restart. The dynamic regulation of RAD51 filament formation and disassembly at replication forks is essential for balancing protection and timely recovery of DNA synthesis. Dysregulation of these processes leads to pathological outcomes\, including chromosomal instability and tumourigenesis. Defects in HR factors\, particularly those controlling RAD51 filament dynamics\, are strongly linked to human diseases such as cancer predisposition syndromes. Understanding how RAD51 orchestrates HR across different DNA damage contexts provides critical insights into genome stability mechanisms and offers therapeutic opportunities\, including synthetic lethality-based cancer treatments. URL:https://www.biomed.cas.cz/event/seminar-lumir-krejci/ LOCATION:Posluchárna Milana Haška / Milan Hašek Auditorium ORGANIZER;CN="%C3%9AMG":MAILTO:leona.krausova@img.cas.cz END:VEVENT BEGIN:VEVENT DTSTART;TZID=Europe/Prague:20260401T150000 DTEND;TZID=Europe/Prague:20260401T160000 DTSTAMP:20260418T213029 CREATED:20260324T121133Z LAST-MODIFIED:20260408T101222Z UID:2510-1775055600-1775059200@www.biomed.cas.cz SUMMARY:Seminář Anna Zitová DESCRIPTION:“Amphioxus point of view” \nLight\, a crucial environmental signal\, that drives lives of myriad creatures upon the Earth\, regulating processes such as circadian rhythms\, reproductive cycles\, and visually guided behaviors. We all perceive light through specialized cells called photoreceptors. Behold Amphioxus\, who possesses several distinct types of photoreceptors. It is also considered one of the closest living likenesses to the last common ancestor of all vertebrates. Although it lacks many vertebrate-specific innovations\, it has a simple body plan and homologous structures shared with vertebrates. In this discourse\, we shall cast our gaze more narrowly upon these photoreceptors of Amphioxus\, to unravel their function and inquire whether they may reveal some glimmer of the origins whence the vertebrate visual systems first took its light. URL:https://www.biomed.cas.cz/event/seminar-anna-zitova/ LOCATION:Posluchárna Milana Haška / Milan Hašek Auditorium ORGANIZER;CN="%C3%9AMG":MAILTO:leona.krausova@img.cas.cz END:VEVENT BEGIN:VEVENT DTSTART;TZID=Europe/Prague:20260325T150000 DTEND;TZID=Europe/Prague:20260325T160000 DTSTAMP:20260418T213029 CREATED:20260316T072314Z LAST-MODIFIED:20260408T101231Z UID:2501-1774450800-1774454400@www.biomed.cas.cz SUMMARY:Seminář Michael Sixt DESCRIPTION:“Mechanic and energetic principles of leukocyte locomotion” \nDuring metazoan development\, immune surveillance and cancer dissemination\, cells migrate in complex three-dimensional (3D) microenvironments. These are crowded by cells and extracellular matrix\, generating mazes of differently sized spaces typically smaller than the diameter of the migrating cell. Most mesenchymal and epithelial cells actively generate their migratory path using pericellular tissue proteolysis and transmit traction forces via specific adhesion receptors. On the contrary\, amoeboid cells such as leukocytes employ non-destructive strategies of locomotion and do not hold on to extracellular substrates. This raises the question how these extremely fast cells negotiate dense tissues. We discovered that leukocytes are able to migrate in the total absence of transmembrane force coupling. Instead\, active deformations of the cell body can impose normal forces on the substrate and thereby generate propulsion. We are actively investigating how these normal forces are triggered and generated by the collective activity of the actin and microtuble cytoskeleton and develop new approaches to measure the energetic demands of the process. URL:https://www.biomed.cas.cz/event/seminar-michael-sixt/ LOCATION:Posluchárna Milana Haška / Milan Hašek Auditorium ORGANIZER;CN="%C3%9AMG":MAILTO:leona.krausova@img.cas.cz END:VEVENT BEGIN:VEVENT DTSTART;TZID=Europe/Prague:20260318T150000 DTEND;TZID=Europe/Prague:20260318T160000 DTSTAMP:20260418T213029 CREATED:20260305T070841Z LAST-MODIFIED:20260408T101238Z UID:2499-1773846000-1773849600@www.biomed.cas.cz SUMMARY:Seminář Srikant Ojha DESCRIPTION:“Plectin Loss Disrupts Mechanotransduction and Attenuates\nHepatic Stellate Cell Activation” \nLiver fibrosis\, a hallmark of fibroproliferative disorders that contribute to nearly 45% of global mortality\, is primarily driven by the activation of hepatic stellate cells (HSCs). In response to injury-induced tissue stiffening\, HSCs undergo mechanosignaling-dependent activation and deposit excessive extracellular matrix (ECM)\, perpetuating a self-sustaining fibrotic loop. We sought to disrupt this process by targeting plectin\, a cytolinker protein central to mechanotransduction. Using an HSC-specific plectin knockout (KO) mouse model\, we observed significantly reduced ECM accumulation following carbon tetrachloride–induced injury. In vitro\, plectin-deficient HSCs displayed impaired proliferation\, migration\, and focal adhesion formation. Single-cell transcriptomics further revealed attenuated activation signatures in KO HSCs. Together\, our findings establish plectin as a key regulator of HSC mechanosignaling and highlight its potential as a therapeutic target to alleviate liver fibrosis. URL:https://www.biomed.cas.cz/event/seminar-srikant-ojha/ LOCATION:Posluchárna Milana Haška / Milan Hašek Auditorium ORGANIZER;CN="%C3%9AMG":MAILTO:leona.krausova@img.cas.cz END:VEVENT BEGIN:VEVENT DTSTART;TZID=Europe/Prague:20260311T150000 DTEND;TZID=Europe/Prague:20260311T160000 DTSTAMP:20260418T213029 CREATED:20260302T081531Z LAST-MODIFIED:20260302T081531Z UID:2496-1773241200-1773244800@www.biomed.cas.cz SUMMARY:Seminář Diego André Florian Joseph DESCRIPTION:“The Fall of the Helicase of Dicer” \nDicer is an RNase III endoribonuclease that processes pre-miRNAs and dsRNAs into small RNAs of ~21–28 nt\, serving the miRNA and RNAi pathways. In mammals\, Dicer’s ability to process long dsRNA is constrained by its helicase domain. Consequently\, the miRNA pathway predominates\, and RNAi is considered vestigial. This constraint comes from a crucial interaction between the HEL1 subdomain of Dicer and its catalytic domain\, which maintains Dicer in a “closed” conformation\, making it highly selective. In the absence of HEL1\, Dicer adopts an “open” conformation that reduces substrate selectivity and activates RNAi. Until now\, this was the only interaction known to confer substrate selectivity and restrict RNAi activity. Here\, we show that Dicer contains an additional region within its helicase that contributes to substrate selectivity. Analysis of Dicer’s AlphaFold structure revealed four major intrinsically disordered regions (IDRs) adopting loop-like conformations. IDR1 is enriched in negatively charged residues and is predicted to interact with the positively charged RNA substrate channel of Dicer. Removal of IDR1 recapitulates the phenotype observed in the absence of HEL1\, suggesting that HEL1 subdomain is not the only region in Dicer responsible for substrate selectivity. URL:https://www.biomed.cas.cz/event/seminar-diego-andre-florian-joseph/ LOCATION:Posluchárna Milana Haška / Milan Hašek Auditorium ORGANIZER;CN="%C3%9AMG":MAILTO:leona.krausova@img.cas.cz END:VEVENT BEGIN:VEVENT DTSTART;TZID=Europe/Prague:20260304T150000 DTEND;TZID=Europe/Prague:20260304T160000 DTSTAMP:20260418T213029 CREATED:20260225T130603Z LAST-MODIFIED:20260225T130603Z UID:2494-1772636400-1772640000@www.biomed.cas.cz SUMMARY:Seminář Vladimír Varga DESCRIPTION:“Towards understanding life cycle of Trypanosoma brucei” \nTrypanosoma brucei is a unicellular eukaryotic parasite of mammals causing sleeping sickness in humans. T. brucei is transmitted by the tsetse fly. In the fly trypanosomes migrate from the gut to salivary glands\, which is associated with a defined sequence of changes to cells of the parasite. We have established an in vitro approach enabling us for the first time to study these life cycle transitions during initial stages of trypanosome migration. Hence\, we were able to describe associated changes to trypanosome morphology\, cell cycle\, motility as well as to its proteome and metabolome. This revealed that the initial life cycle transitions represent a continuous plastic process rather than a sequence of irreversible decisions. Furthermore\, it let to identification of a molecule in the trypanosome environment\, which is critical for triggering cell cycle arrest during these transitions. Thus\, our work opens new possibilities to understand complex biology of this important human parasite. URL:https://www.biomed.cas.cz/event/seminar-vladimir-varga/ LOCATION:Posluchárna Milana Haška / Milan Hašek Auditorium ORGANIZER;CN="%C3%9AMG":MAILTO:leona.krausova@img.cas.cz END:VEVENT BEGIN:VEVENT DTSTART;VALUE=DATE:20260303 DTEND;VALUE=DATE:20260304 DTSTAMP:20260418T213029 CREATED:20260223T091618Z LAST-MODIFIED:20260223T091618Z UID:2491-1772496000-1772582399@www.biomed.cas.cz SUMMARY:BD Biosciences – Lunch and Learn Seminar DESCRIPTION:Dear colleagues\, \nWe would like to invite you to join Lunch & Learn seminar focused on Panel Design Strategy in Flow Cytometry. This session is designed to support anyone involved in planning\, optimizing\, or performing multicolor flow cytometry experiments by providing both conceptual guidance and practical demonstrations. \n  \nMore information and registration. \nFlyer \n  \nRegistration is free. \n  \nBest regards \n  \n— \n\n\n\n\n\n\nMatyáš Šíma\, Ph.D. URL:https://www.biomed.cas.cz/event/bd-biosciences-lunch-and-learn-seminar/ END:VEVENT BEGIN:VEVENT DTSTART;TZID=Europe/Prague:20260225T150000 DTEND;TZID=Europe/Prague:20260225T160000 DTSTAMP:20260418T213029 CREATED:20260216T074449Z LAST-MODIFIED:20260216T074449Z UID:2486-1772031600-1772035200@www.biomed.cas.cz SUMMARY:Seminář Poulami Banik DESCRIPTION:“Characterization and targeted rescue of a novel PRPF31 mutation in retinitis pigmentosa” \nRetinitis pigmentosa (RP) is an inherited retinal disease characterized by photoreceptor loss. Mutations in the PRPF31 gene cause approximately 10% of cases of autosomal dominant RP. In this project\, we characterize a novel intronic pathogenic variant in the PRPF31 gene. We provide evidence that the novel RP mutation activates a cryptic splice site\, inducing the expression of an abnormal transcript. The resulting protein is unstable\, and patient cells exhibit lower expression of PRPF31 and other splicing factors. To correct aberrant RNA splicing\, we applied modified antisense oligonucleotides (ASOs) to improve PRPF31 splicing in patient cells. We differentiated the patients‘ induced pluripotent stem cells (iPSCs) into retinal pigment epithelium (RPE) cells and demonstrated that ASO treatment improves PRPF31 splicing and increases PRPF31 protein levels in patient’s RPE cells. These findings suggest that ASO-based splicing correction is a promising therapy for this currently untreatable disease. URL:https://www.biomed.cas.cz/event/seminar-poulami-banik/ LOCATION:Posluchárna Milana Haška / Milan Hašek Auditorium ORGANIZER;CN="%C3%9AMG":MAILTO:leona.krausova@img.cas.cz END:VEVENT BEGIN:VEVENT DTSTART;TZID=Europe/Prague:20260218T150000 DTEND;TZID=Europe/Prague:20260218T160000 DTSTAMP:20260418T213029 CREATED:20260209T151442Z LAST-MODIFIED:20260209T151442Z UID:2484-1771426800-1771430400@www.biomed.cas.cz SUMMARY:Seminář Pranav Gulati DESCRIPTION:“Initiating Mechanisms of Autoinflammatory Osteomyelitis: dysregulated neutrophil recruitment and tyrosine kinase signaling” \nAutoinflammatory diseases result from inappropriate activation of the innate immune system in the absence of infection. Loss of PSTPIP2\, a membrane adaptor protein\, leads to the development of one such disease Chronic Multifocal Osteomyelitis (CMO)\, an autoinflammatory disease in mice which serves as a model for Chronic Recurrent Multifocal Osteomyelitis (CRMO) in humans. Neutrophils are essential for murine CMO initiation and contribute to tissue damage\, but the early events that trigger their pathogenic activity remain poorly defined. Our work shows that early alterations in neutrophil signaling and migration precede both classical inflammatory cytokine responses and the appearance of overt disease symptoms. Pharmacological inhibition of some of these early pathways reduces disease severity and neutrophil activation. Together\, these findings identify the initiating mechanisms of sterile bone inflammation in the mouse model of autoinflammatory osteomyelitis and provide potential targets for therapeutic intervention. URL:https://www.biomed.cas.cz/event/seminar-pranav-gulati/ LOCATION:Posluchárna Milana Haška / Milan Hašek Auditorium ORGANIZER;CN="%C3%9AMG":MAILTO:leona.krausova@img.cas.cz END:VEVENT BEGIN:VEVENT DTSTART;TZID=Europe/Prague:20260211T150000 DTEND;TZID=Europe/Prague:20260211T160000 DTSTAMP:20260418T213029 CREATED:20260129T104523Z LAST-MODIFIED:20260129T104523Z UID:2482-1770822000-1770825600@www.biomed.cas.cz SUMMARY:Seminář Lucie Janečková DESCRIPTION:“NG2-lineage cell plasticity in ischemic brain repair: Roles of Wnt signaling” \nFocal cerebral ischemia (FCI) leads to neuronal loss followed by extensive tissue remodeling\, in which glial and vascular cell populations play indispensable roles. Among these\, NG2 glia and perivascular NG2⁺ cells represent highly plastic cell types that actively participate in post-ischemic responses\, yet their functional heterogeneity and regulatory mechanisms remain poorly understood. In this lecture\, I will present our work investigating how these cell populations respond to ischemic injury and how modulation of Wnt/β-catenin signaling reshapes their cellular states and functions. By combining lineage tracing\, transcriptomic analyses\, and imaging approaches\, we uncover changes in oligodendrocyte differentiation\, perivascular cell behavior\, and their contributions to tissue repair and regeneration. Finally\, I will introduce an ongoing spatial transcriptomics study aimed at mapping cellular organization in the ischemic periphery and glial scar\, highlighting emerging spatial patterns and open questions in brain regeneration. URL:https://www.biomed.cas.cz/event/seminar-lucie-janeckova/ LOCATION:Posluchárna Milana Haška / Milan Hašek Auditorium ORGANIZER;CN="%C3%9AMG":MAILTO:leona.krausova@img.cas.cz END:VEVENT BEGIN:VEVENT DTSTART;TZID=Europe/Prague:20260204T150000 DTEND;TZID=Europe/Prague:20260204T160000 DTSTAMP:20260418T213029 CREATED:20260115T104013Z LAST-MODIFIED:20260115T104013Z UID:2480-1770217200-1770220800@www.biomed.cas.cz SUMMARY:Seminář Susana Minguet DESCRIPTION:“Harnessing TCR Discoveries for the Next Generation of Immunotherapies” \nRecent advances in synthetic immunology and CAR T cell engineering have highlighted the critical importance of signaling diversity for optimizing anti-tumor responses. Our work outlines the design of innovative cell-based cancer immunotherapies rooted in a molecular understanding of T cell activation via the T cell receptor complex (TCR–CD3). I will discuss how modulating receptor signaling interfaces and precisely recruiting kinases and adaptors can advance both fundamental immunology and translational therapies. Key noncanonical interactions between specific components of the TCR–CD3 complex and the Src-kinase Lck fine-tune T cell activation; when implemented in chimeric antigen receptors (CARs)\, these mechanisms improved in vivo tumor control and reduced T cell exhaustion. Supporting our rational design of next-generation CAR T cells\, we demonstrated that harnessing CD3 chain diversity\, rather than simply increasing signaling strength\, enabled the generation of CAR T cells with enhanced anti-tumor efficacy and safety profiles. URL:https://www.biomed.cas.cz/event/seminar-susana-minguet/ LOCATION:Posluchárna Milana Haška / Milan Hašek Auditorium ORGANIZER;CN="%C3%9AMG":MAILTO:leona.krausova@img.cas.cz END:VEVENT BEGIN:VEVENT DTSTART;TZID=Europe/Prague:20260128T150000 DTEND;TZID=Europe/Prague:20260128T160000 DTSTAMP:20260418T213029 CREATED:20260112T073717Z LAST-MODIFIED:20260112T073717Z UID:2474-1769612400-1769616000@www.biomed.cas.cz SUMMARY:Seminář Lorena Arranz DESCRIPTION:“Therapeutic targeting of stem cells and their niches in myeloid malignancies” \nOur research group “Stem Cells\, Ageing and Cancer” aims at providing insights into mechanisms of hematopoietic stem cell malignant transformation\, including interactions of this cell with its surrounding microenvironment\, and a focus on inflammation\, signaling oncometabolites and various cellular components of the stem cell niche. Our goal is the identification of novel therapeutic targets of potential clinical interest for myeloid malignancies. \nWe will give an overview of our research and present unpublished observations accepted in principle in Nature Communications\, where we have uncovered an important role for the metabolite succinate and its membrane receptor Succinate receptor (Sucnr1) in the regulation of hematopoiesis in health and malignancy. Our work suggests that Sucnr1 signaling restricts hematopoiesis at least partially via the hematopoietic stem and progenitor cell compartment and control of S100a9. The dysregulation of Sucnr1 emerges as contributor to malignancy\, accounting for SUCNR1 expression as a predictor of prognosis in acute myeloid leukemia (AML) patients and opening new potential therapeutic avenues for these patients. URL:https://www.biomed.cas.cz/event/seminar-lorena-arranz/ LOCATION:Posluchárna Milana Haška / Milan Hašek Auditorium ORGANIZER;CN="%C3%9AMG":MAILTO:leona.krausova@img.cas.cz END:VEVENT BEGIN:VEVENT DTSTART;TZID=Europe/Prague:20260127T150000 DTEND;TZID=Europe/Prague:20260127T160000 DTSTAMP:20260418T213029 CREATED:20260113T133922Z LAST-MODIFIED:20260113T133922Z UID:2478-1769526000-1769529600@www.biomed.cas.cz SUMMARY:Seminář Hanna-Mari Baldauf DESCRIPTION:“From virus-host interactions to a novel protein delivery tool” \nThe VIIRAL lab has advanced the understanding of species-specific virus-host interactions against retroviruses. This advancement facilitates now the development of a rabbit model against HIV\, which is a long standing interest in the lab. We use virological\, cellular\, biochemical\, and evolutionary approaches to decipher motifs/pathways that might be important for virus-host interactions. Furthermore\, we have developed VICTORI\, a novel protein delivery platform that can be tailored for the delivery of therapeutic proteins for different purposes. \n  URL:https://www.biomed.cas.cz/event/seminar-hanna-mari-baldauf/ LOCATION:Posluchárna 0.195 / Lecture room 0.195 ORGANIZER;CN="%C3%9AMG":MAILTO:leona.krausova@img.cas.cz END:VEVENT BEGIN:VEVENT DTSTART;TZID=Europe/Prague:20260121T150000 DTEND;TZID=Europe/Prague:20260121T160000 DTSTAMP:20260418T213029 CREATED:20260112T143738Z LAST-MODIFIED:20260112T143738Z UID:2476-1769007600-1769011200@www.biomed.cas.cz SUMMARY:Seminář Anna Koslová DESCRIPTION:“Integration and reactivation of endogenous DNA viruses in unicellular eukaryotes” \nViruses infecting protists play important ecological and evolutionary roles by modulating microbial population dynamics and contributing to genome evolution through endogenization. Protist genomes contain tens to thousands of endogenous viral elements (EVEs)\, many of which appear intact and may represent functional viruses. Some EVEs are related to virophages—viruses that parasitize on larger “giant viruses” infecting eukaryotic hosts. Using an isolated virophage system\, we aim to uncover the molecular mechanisms underlying viral DNA integration and reactivation. URL:https://www.biomed.cas.cz/event/seminar-anna-koslova/ LOCATION:Posluchárna Milana Haška / Milan Hašek Auditorium ORGANIZER;CN="%C3%9AMG":MAILTO:leona.krausova@img.cas.cz END:VEVENT BEGIN:VEVENT DTSTART;TZID=Europe/Prague:20260114T150000 DTEND;TZID=Europe/Prague:20260114T160000 DTSTAMP:20260418T213029 CREATED:20260105T115716Z LAST-MODIFIED:20260105T121039Z UID:2471-1768402800-1768406400@www.biomed.cas.cz SUMMARY:Seminář Viktor Bugajev DESCRIPTION:“Multiple Layers of Control in Early Sphingolipid Biosynthesis” \nSphingolipids are fundamental components of cellular membranes and key mediators of intracellular signaling. Their de novo synthesis is initiated in the endoplasmic reticulum (ER) and is tightly regulated at the level of the serine palmitoyltransferase (SPT) complex. ORMDL proteins are established canonical negative regulators of SPT activity\, thereby maintaining sphingolipid homeostasis. In this seminar\, we demonstrate that ceramides act as major regulators of ORMDL expression levels and identify a previously unrecognized ORMDL interactor that establishes a novel relationship between ORMDL expression and sphingolipid abundance\, reminiscent of dysregulated sphingolipid metabolism observed in pathological conditions. These findings uncover an additional layer of control over the sphingolipid pathway and highlight unexpected flexibility in ORMDL-dependent regulation of de novo sphingolipid biosynthesis. URL:https://www.biomed.cas.cz/event/seminar-viktor-bugajev/ LOCATION:Posluchárna Milana Haška / Milan Hašek Auditorium ORGANIZER;CN="%C3%9AMG":MAILTO:leona.krausova@img.cas.cz END:VEVENT BEGIN:VEVENT DTSTART;TZID=Europe/Prague:20260107T150000 DTEND;TZID=Europe/Prague:20260107T160000 DTSTAMP:20260418T213029 CREATED:20251216T124552Z LAST-MODIFIED:20251216T124552Z UID:2469-1767798000-1767801600@www.biomed.cas.cz SUMMARY:Seminář Veronika Niederlová DESCRIPTION:“CD8+ T cells in infection: Orchestration of the effector and memory fates” \nIn infection\, naive CD8+ T cells differentiate into short-lived effector cells\, which provide immediate protection against the pathogen\, and long-lived memory cells\, which retain immunological information long after pathogen clearance. Using single-cell multiomics techniques and in vivo tracking of individual clonal families\, we show which factors drive the effector versus memory cell fate decision. \n  URL:https://www.biomed.cas.cz/event/seminar-veronika-niederlova/ LOCATION:Posluchárna Milana Haška / Milan Hašek Auditorium ORGANIZER;CN="%C3%9AMG":MAILTO:leona.krausova@img.cas.cz END:VEVENT BEGIN:VEVENT DTSTART;TZID=Europe/Prague:20251217T150000 DTEND;TZID=Europe/Prague:20251217T160000 DTSTAMP:20260418T213029 CREATED:20251202T073428Z LAST-MODIFIED:20251202T073428Z UID:2467-1765983600-1765987200@www.biomed.cas.cz SUMMARY:Seminář Mehak Nihal Shaikh DESCRIPTION:“ALDH1A1 inhibition as a therapeutic target in Acute Myeloid Leukemia” \nAcute Myeloid Leukemia (AML) is a hematological malignancy leading to differentiation arrest of the myeloid compartment in the bone marrow. It is reported that high levels of ALDH1A1 inhibition promote chemoresistance in AML. Therefore\, in my project we are studying the role of ALDH1A1 inhibition in AML using AML cell lines\, mouse models\, and AML patient samples. And lastly\, we will show the effect of ALDH1A1 inhibition in combination with standard of care chemotherapeutic drugs as a potential treatment strategy for AML. \n  URL:https://www.biomed.cas.cz/event/seminar-mehak-nihal-shaikh/ LOCATION:Posluchárna Milana Haška / Milan Hašek Auditorium ORGANIZER;CN="%C3%9AMG":MAILTO:leona.krausova@img.cas.cz END:VEVENT BEGIN:VEVENT DTSTART;TZID=Europe/Prague:20251210T150000 DTEND;TZID=Europe/Prague:20251210T160000 DTSTAMP:20260418T213029 CREATED:20251202T071927Z LAST-MODIFIED:20251202T071927Z UID:2465-1765378800-1765382400@www.biomed.cas.cz SUMMARY:Seminář Jakub Rídl DESCRIPTION:“Enigmatic germline-restricted chromosome of songbirds has different centromere compared to regular chromosomes” \nCentromeres are an important part of chromosomes which direct chromosome segregation during cell division. Their modifications can therefore explain the unusual mitotic and meiotic behaviour of certain chromosomes\, such as the germline-restricted chromosome (GRC) of songbirds. This chromosome is eliminated from somatic cells during early embryogenesis and later also from male germ cells during spermatogenesis. We used a combination of cytogenetic and genomic approaches to identify the centromeric sequences of two closely related songbird species\, the common nightingale (Luscinia megarhynchos) and the thrush nightingale (L. luscinia). We found a 436-bp satellite repeat present in the centromeric regions of all regular chromosomes (i.e.\, autosomes and sex chromosomes). Interestingly\, hybridization of the probe to this repeat on meiotic spreads suggested that this repeat is missing on the GRC. Our results indicate that the change of the centromeric sequence may underlie the unusual inheritance and programmed DNA elimination of the GRC in songbirds. URL:https://www.biomed.cas.cz/event/seminar-jakub-ridl/ LOCATION:Posluchárna Milana Haška / Milan Hašek Auditorium ORGANIZER;CN="%C3%9AMG":MAILTO:leona.krausova@img.cas.cz END:VEVENT BEGIN:VEVENT DTSTART;TZID=Europe/Prague:20251203T150000 DTEND;TZID=Europe/Prague:20251203T160000 DTSTAMP:20260418T213029 CREATED:20251124T135541Z LAST-MODIFIED:20251124T135541Z UID:2463-1764774000-1764777600@www.biomed.cas.cz SUMMARY:Seminář Tomáš Venit DESCRIPTION:“Nuclear myosin 1 – from gene to function” \nMitochondria play a vital role in cellular metabolism by generating energy through oxidative phosphorylation (OXPHOS) in most somatic cells. However\, highly proliferative\, undifferentiated pluripotent stem cells and cancer cells mainly rely on aerobic glycolysis for energy production. Recently\, we reported that nuclear myosin 1 (NM1) functions as a tumor suppressor and a key regulator of cellular metabolism\, directly controlling the expression of the mitochondrial transcription factors TFAM and Pgc1α. Its deletion alters mitochondrial structure\, reduces OXPHOS gene expression\, induces a metabolic shift toward aerobic glycolysis\, and promotes tumor formation in mice. In this talk\, I will move from tumor development to somatic tissues and share our latest findings from phenotyping NM1 knockout mice. URL:https://www.biomed.cas.cz/event/seminar-tomas-venit/ LOCATION:Posluchárna Milana Haška / Milan Hašek Auditorium ORGANIZER;CN="%C3%9AMG":MAILTO:leona.krausova@img.cas.cz END:VEVENT BEGIN:VEVENT DTSTART;TZID=Europe/Prague:20251201T150000 DTEND;TZID=Europe/Prague:20251201T150000 DTSTAMP:20260418T213029 CREATED:20251020T111027Z LAST-MODIFIED:20251020T111314Z UID:2429-1764601200-1764601200@www.biomed.cas.cz SUMMARY:Metabolomics and lipidomics: platforms\, pitfalls\, and practical tips DESCRIPTION:Doc. Ing. Tomáš Čajka Ph.D.\, Fyziologický ústav AV ČR\, bude zajišťovat  21 th Metodologický seminář Krč – Biocev na téma: \n„Metabolomics and lipidomics: platforms\, pitfalls\, and practical tips“\, který se bude konat 1.12.2025 od 15\,00 hodin v Kinosále FGÚ. \nAnotace: \nMetabolomics and lipidomics are rapidly advancing fields that have transformed our understanding of biological processes at the molecular level. Yet\, designing an appropriate workflow and selecting from the many available analytical options can be challenging [1]. Since 2018\, the Metabolomics Core Facility at the Institute of Physiology CAS (https://metabolomics.fgu.cas.cz) has offered fee-based services for analyzing polar metabolites\, complex lipids\, and exposome compounds (including drugs) in biological materials [2]. The scope of these services has steadily expanded\, with approximately 5\,000 samples analyzed annually using a multiplatform liquid chromatography–mass spectrometry (LC–MS) approach. This lecture will present the current untargeted LC–MS platforms (LIMeX workflow) and introduce upcoming platforms. We will also discuss common pitfalls related to study design\, metabolome coverage\, and statistical analysis. \n[1] Rakusanova & Cajka\, Trends Anal Chem 180 (2024) 117940 (doi: 10.1016/j.trac.2024.117940) \n[2] Cajka et al.\, Int J Mol Sci 324 (2023) 1987 (doi: 10.3390/ijms24031987) URL:https://www.biomed.cas.cz/event/metabolomics-and-lipidomics-platforms-pitfalls-and-practical-tips/ LOCATION:Kinosal\, Vídeňská 1083\, Praha ORGANIZER;CN="FGU":MAILTO:Olga.Zimmermannova@fgu.cas.cz END:VEVENT BEGIN:VEVENT DTSTART;VALUE=DATE:20251201 DTEND;VALUE=DATE:20251204 DTSTAMP:20260418T213029 CREATED:20251107T103704Z LAST-MODIFIED:20251107T103704Z UID:2438-1764547200-1764806399@www.biomed.cas.cz SUMMARY:Course on scRNA-seq Data Analysis - IMG DESCRIPTION:Single-cell RNA sequencing (scRNA-seq) allows researchers to study gene expression at the level of individual cells. This approach can\, for example\, help to identify different cell populations in a complex sample and describe their expression patterns. To generate and analyse scRNA-seq data\, several methods are available\, all with their strengths and weaknesses depending on the researchers’ needs. This 3-day course will cover the main technologies as well as the main aspects to consider while designing an scRNA-seq experiment. In particular\, it will combine the theoretical background of analytical methods with hands-on data analysis sessions focused on data generated by droplet-based platforms.\n\nBy the end of the course\, participants will possess the following abilities:\n • Distinguish advantages and pitfalls of scRNA-seq.\n • Design their own scRNA-seq experiment\, using common technologies like 10× Genomics.\n • Apply quality control (QC) measures and utilise analysis tools to preprocess scRNA-seq data.\n • Apply normalisation\, scaling\, dimensionality reduction\, integration and clustering on scRNA-seq data using R.\n • Differentiate between cell annotation techniques to identify and characterise cell populations.\n • Use differential gene expression analysis methods on scRNA-seq data to gain biological insights.\n • Select enrichment analysis methods appropriate to the biological question and data.\n • Develop an scRNA-seq data analysis workflow from raw count matrix to differential gene expression with peer support and light guidance.\n\nMore information and registration at https://www.elixir-czech.cz/events/course-on-scrna-seq-data-analysis-2025\n\nOn behalf of the organisers\,\n\nMichal Kolář\n\n—\nLaboratory of Genomics and Bioinformatics\nInstitute of Molecular Genetics of the Czech Academy of Sciences\nVidenska 1083\n142 20 Prague 4\nCzech Republic\n\nPhone +420 241 063 412\nEmail kolarmi@img.cas.cz URL:https://www.biomed.cas.cz/event/course-on-scrna-seq-data-analysis-img/ LOCATION:Posluchárna 0.195 / Lecture room 0.195 ORGANIZER;CN="%C3%9AMG":MAILTO:leona.krausova@img.cas.cz END:VEVENT BEGIN:VEVENT DTSTART;TZID=Europe/Prague:20251126T150000 DTEND;TZID=Europe/Prague:20251126T160000 DTSTAMP:20260418T213029 CREATED:20251118T072631Z LAST-MODIFIED:20251118T072631Z UID:2461-1764169200-1764172800@www.biomed.cas.cz SUMMARY:Seminář Tomas Paulenda DESCRIPTION:“The immunoregulatory properties of the metabolite itaconate” \nIn this talk I will present my latest findings of the function of immunoregulatory metabolite itaconate. Itaconate accumulates in innate immune cells upon Toll-like receptor stimulation. In response to macrophage activation by lipopolysaccharide\, itaconate inhibits inflammasome activation and boosts type I interferon signalling; however\, the molecular mechanism of this immunoregulation remained unclear. Here\, we show that the enhancement of type I interferon secretion by itaconate depends on the inhibition of peroxiredoxin 5 and on mitochondrial reactive oxygen species. We find that itaconate non-covalently inhibits peroxiredoxin 5\, leading to the modulation of mitochondrial peroxide in activating macrophages. Through genetic manipulation\, we confirm that peroxiredoxin 5 modulates  type I interferon secretion in macrophages. The non-electrophilic itaconate mimetic 2-methylsuccinate inhibits peroxiredoxin 5 and phenocopies immunoregulatory action of itaconate on type I interferon and inflammasome activation\, providing further support for a non-covalent inhibition of peroxiredoxin 5 by itaconate. Our work shows Peroxiredoxin 5 as a novel Type I interferon regulator with big potential to future research of redox regulation of immune responses. URL:https://www.biomed.cas.cz/event/seminar-tomas-paulenda/ LOCATION:Posluchárna Milana Haška / Milan Hašek Auditorium ORGANIZER;CN="%C3%9AMG":MAILTO:leona.krausova@img.cas.cz END:VEVENT BEGIN:VEVENT DTSTART;TZID=Europe/Prague:20251119T150000 DTEND;TZID=Europe/Prague:20251119T160000 DTSTAMP:20260418T213029 CREATED:20251113T085932Z LAST-MODIFIED:20251113T085932Z UID:2459-1763564400-1763568000@www.biomed.cas.cz SUMMARY:Seminář Nándor Nagy DESCRIPTION:“Understanding the enteric nervous system development through the avian embryo model: implications for regenerative biology” \nIn this presentation\, I will provide an overview of enteric nervous system (ENS) development from the perspective of the avian embryo. The ENS\, often referred to as the “second brain\,” originates from vagal neural crest cells that migrate into the developing gut and differentiate into neuronal and glial networks that regulate intestinal function. \nAvian species\, particularly chicken and quail\, have long served as powerful model organisms for studying the cellular and molecular mechanisms regulating ENS formation\, because their embryos are easily accessible\, rapidly developing\, and highly suitable for experimental manipulation. Today\, with new tools such as in ovo electroporation\, retroviral gene transfer\, and CRISPR-based editing\, the avian model again provides unique opportunities to study enteric neurogenesis in vivo. \nIntegrating classical embryonic manipulation approaches with single-cell and spatial transcriptomic analyses now provides a comprehensive framework for understanding enteric neurogenesis\, lineage diversification\, and the evolutionary conservation of neural crest–derived systems across vertebrates. URL:https://www.biomed.cas.cz/event/seminar-nandor-nagy/ LOCATION:Posluchárna Milana Haška / Milan Hašek Auditorium ORGANIZER;CN="%C3%9AMG":MAILTO:leona.krausova@img.cas.cz END:VEVENT BEGIN:VEVENT DTSTART;TZID=Europe/Prague:20251118T093000 DTEND;TZID=Europe/Prague:20251118T123000 DTSTAMP:20260418T213029 CREATED:20251013T070142Z LAST-MODIFIED:20251013T070142Z UID:2421-1763458200-1763469000@www.biomed.cas.cz SUMMARY:FlowJo™ 11 training DESCRIPTION:Dear colleagues\, \non November 18th 2025 we will organize FlowJo™ 11 training with Ioannis Panetas\, Ph.D.- application specialist from BD. \nFlowJo is a powerful cytometry data analysis software available to users of our Flow Cytometry Core Facility. \nMore information about FlowJo: https://www.flowjo.com/solutions/flowjo \nThis year\, the training will focus on the new version 11\, which\, in terms of the user interface and some features\, is quite different from the previous version. This training is therefore also suitable for more experienced FlowJo users who have not yet become familiar with version 11. \n  \nTuesday November 18th 2025\, 9:30 am — 12:30pm \nInstitute of Molecular Genetics of the Czech Academy of Sciences \nVídeňská 1083\n142 20 Prague 4\, Czech Republic \nHašek Auditorium \n  \n9:30 AM – 10:15 AM | Unveiling FlowJo 11\, the future of data analysis\nCome hear about the newest version of FlowJo Software! We’ll tour the new immersive workbench and discuss features unique to this version such as the Metadata Manager\, Graph Gallery\, virtual concatenation\, charts\, and integrated algorithms. \n10:30 AM – 12:30 PM | Hands-on training for FlowJo 11\nAttendees will be guided through the analysis of a shared data set. To participate\, users are expected to bring their own laptop\, complete all downloads and installs\, and have an active FlowJo Portal license. Please follow the instructions here \n  \nIf you don‘ have license on your laptop\, please check yes in the related question in registration form and BD will send you temporary license. \n  \nThere is no fee for the training. \n  \nIf you want to attend\, please fill the registration form: https://forms.gle/23UrU6fmdn7TxSxCA \nBest regards \nMatyáš Šíma URL:https://www.biomed.cas.cz/event/flowjo-11-training/ LOCATION:Posluchárna Milana Haška / Milan Hašek Auditorium ORGANIZER;CN="%C3%9AMG":MAILTO:leona.krausova@img.cas.cz END:VEVENT BEGIN:VEVENT DTSTART;VALUE=DATE:20251118 DTEND;VALUE=DATE:20251122 DTSTAMP:20260418T213029 CREATED:20250923T132640Z LAST-MODIFIED:20250923T132716Z UID:2411-1763424000-1763769599@www.biomed.cas.cz SUMMARY:Single-cell course – laboratory methods DESCRIPTION:Dear Colleagues\,\n\nWe are excited to invite you to an intensive introductory three-day course on Single-cell analysis - from qPCR to sequencing\, organised by the Institute of Biotechnology (Radek Šindelka) and the Institute of Molecular Genetics (Michal Kolář).\n\nDates: November 18\, 19\, and 21\nTime: 9:00 AM – 3:00 PM\nLocation: Krč Campus (IMG Lecture room 0.195 – Jágr’s hall)\n\nThis course will provide valuable insights and practical skills in the rapidly evolving field of laboratory methods for single-cell analyses and spatial transcriptomics. With a maximum of 25 participants\, you’ll receive personalised attention and the opportunity to engage deeply with the subject.\n\nIn early December\, a course on bioinformatical analysis will follow up with particular focus on single-cell RNA-seq data.\n\nIf you’re interested in joining us\, please respond via email to sindelka@ibt.cas.cz as soon as possible\, as space is limited.\n\nWe look forward to an enriching experience together!\n\nBest regards\,\n\nRadek Šindelka & Michal Kolář URL:https://www.biomed.cas.cz/event/single-cell-course-laboratory-methods/ LOCATION:Posluchárna 0.195 / Lecture room 0.195 END:VEVENT BEGIN:VEVENT DTSTART;TZID=Europe/Prague:20251112T150000 DTEND;TZID=Europe/Prague:20251112T160000 DTSTAMP:20260418T213029 CREATED:20251103T081947Z LAST-MODIFIED:20251103T081947Z UID:2433-1762959600-1762963200@www.biomed.cas.cz SUMMARY:Seminář Igor Adameyko DESCRIPTION:“The atlas of placodal development helps to decipher their evolutionary origin” \nIn this talk I will present our single cell and spatial transcriptomics data\, which I will place into the light of the current ideas about the placodal evolution. Neurogenic placodes are specialized embryonic ectodermal thickenings in the head that give rise to sensory neurons\, sensory receptor and glial cell\, and endocrine tissues. They\, together with the neural crest\, form the basis of the vertebrate cranial sensory and neurosecretory systems. Placodes are unique to vertebrates and represent a major evolutionary innovation that allowed the elaboration of the vertebrate „new head“ – the complex sensory apparatus and associated ganglia that support the information flows necessary for effective predation and movement. They may have evolved from ancestral epidermal sensory cell fields present in protochordates (like tunicates or amphioxus). URL:https://www.biomed.cas.cz/event/seminar-igor-adameyko/ LOCATION:Posluchárna Milana Haška / Milan Hašek Auditorium ORGANIZER;CN="%C3%9AMG":MAILTO:leona.krausova@img.cas.cz END:VEVENT BEGIN:VEVENT DTSTART;TZID=Europe/Prague:20251105T150000 DTEND;TZID=Europe/Prague:20251105T160000 DTSTAMP:20260418T213029 CREATED:20250925T102537Z LAST-MODIFIED:20250925T102537Z UID:2416-1762354800-1762358400@www.biomed.cas.cz SUMMARY:Seminář Ludger Klein DESCRIPTION:“Role of B cells in central T cell tolerance” \nTolerance to self-antigens is a fundamental property of the immune system. During central tolerance induction in the thymus\, autoreactive T cells are either eliminated or diverted into the regulatory T cell lineage. Both processes are dependent on the presentation of self-antigens by thymic antigen presenting cells (APCs). Commonly\, dendritic cells and medullary thymic epithelial cells are believed to represent the key APCs for central T cell tolerance. The thymus also harbours a distinct population of B cells\, raising the possibility that thymic B cells serve a non-redundant APC function in central T cell tolerance induction. I will discuss our ongoing work to test this hypothesis through large scale comparisons of the TCR composition of ‘normally’ selected T cell repertoires versus repertoires selected in the absence of B cells or Dendritic cells or both. URL:https://www.biomed.cas.cz/event/seminar-ludger-klein/ LOCATION:Posluchárna Milana Haška / Milan Hašek Auditorium ORGANIZER;CN="%C3%9AMG":MAILTO:leona.krausova@img.cas.cz END:VEVENT BEGIN:VEVENT DTSTART;VALUE=DATE:20251103 DTEND;VALUE=DATE:20251115 DTSTAMP:20260418T213029 CREATED:20250912T070739Z LAST-MODIFIED:20250912T070739Z UID:2406-1762128000-1763164799@www.biomed.cas.cz SUMMARY:49th Advances in Molecular Biology & Genetics DESCRIPTION:Dear colleagues\, \n\nI would like to invite you to register online for the seminar course Advances in Molecular Biology and Genetics and share news concerning the course. The program of the 49th course is available here:\n\nhttps://pokroky.img.cas.cz/programme/\n\nThe course is a two week lecture series and is particularly suitable for individual study plans (ISPs) of the first year PhD students in biomedicine as lectures cover broad area of molecular biology and biomedical research. The course is accredited at the Charles University as MPGS0034 (https://is.cuni.cz/studium/eng/predmety/index.php?do=predmet&kod=MPGS0034). Therefore\, attending the seminar course is recognized as a fulfilled study requirement in PhD programs in biomedicine at the Charles University. In the past\, some students came from the University of Chemistry and Technology (VSCHT) and the Czech University of Life Sciences (CZU). The course is held in English and will take place in Hasek lecture hall at IMG from November 3rd until 14th\, 2025.\n\nFor attending the course\, we kindly (but firmly) ask all participants to register at the site of the course:\n\nhttps://event.img.cas.cz/registration/advances2025/\n\nThe course is for free\, i.e. there is no registration fee. Please\, note that this registration is in addition to the course registrations/recordings at universities in PhD programs. It is for us and it is necessary\, so we can have a list of all attending students before the meeting starts. Then we can make sure that there is enough cakes during coffee breaks and can keep track of attendance\, which is needed for the credit.\n\nFurther details can be found at the website of the course: https://pokroky.img.cas.cz/\n\nIf you have any questions\, please\, contact: pokroky@img.cas.cz\n\nWith kind regards \nPetr Svoboda & Jiri Jonak on behalf of the organizing team URL:https://www.biomed.cas.cz/event/49th-advances-in-molecular-biology-genetics/ LOCATION:Posluchárna Milana Haška / Milan Hašek Auditorium ORGANIZER;CN="%C3%9AMG":MAILTO:leona.krausova@img.cas.cz END:VEVENT BEGIN:VEVENT DTSTART;VALUE=DATE:20251031 DTEND;VALUE=DATE:20251101 DTSTAMP:20260418T213029 CREATED:20250306T130439Z LAST-MODIFIED:20250306T130439Z UID:2322-1761868800-1761955199@www.biomed.cas.cz SUMMARY:RNA Club DESCRIPTION: URL:https://www.biomed.cas.cz/event/rna-club/ LOCATION:Posluchárna Milana Haška / Milan Hašek Auditorium END:VEVENT END:VCALENDAR