Seminář Lenka Ungrová, M.Sc.
2025-02-05 15:00 - 16:00 (Europe/Prague) | Posluchárna Milana Haška / Milan Hašek Auditorium
“Birds do it their way: avian interferon regulatory factors (almost) resolved”
Interferon regulatory factors (IRFs) are transcription factors that play a key role in vertebrate innate antiviral immunity. IRF3 and IRF7 activate the expression of IFN-β, which in response induces the assembly of STAT1, STAT2 and IRF9 into the ISGF3 complex. Subsequently, this complex activates the expression of interferon-stimulated genes (ISGs) mounting the antiviral state of the cell. Interestingly, IRF9 and IRF3 have been reported to be missing in birds. As part of our efforts to search for hidden genes in avian genomes, we have identified IRF3 in paleognathous birds and IRF9 in most bird orders with the notable exception of the chicken. We focus on the experimental characterization of IRF9 in a duck cell line and also look for a possible substitute for IRF9 in chickens.

Seminář - Prof. Cheng Zhu - "Mechanotransduction through immunoreceptors"
2025-02-06 14:00 - 15:00 | Posluchárna Milana Haška / Milan Hašek Auditorium
Dear colleagues,
I am delighted to invite you to a seminar by Prof. Cheng Zhu (Georgia Institute of Technology, USA) at the Institute of Molecular Genetics on February 6 at 14:00 (Hasek Lecture Hall). Prof. Zhu is a recognized leader and pioneer at the interface of immunology, structural biology, and mechanosensing with groundbreaking contributions that have advanced our understanding of how immune receptors work at the cell-cell contacts.
Invitation: https://www.img.cas.cz/files/2025/01/20250206_Cheng_Zhu.pdf
Publication profile: https://scholar.google.com/citations?user=_tPv2wEAAAAJ&hl=en&oi=ao
With best regards,
Ondrej Stepanek

Školení Genderová dimenze v obsahu výzkumu
2025-02-11 09:00 - 12:00 | Posluchárna Milana Haška / Milan Hašek Auditorium
Seminář povedou Eva Oliva a Gabriela Langhammerová z NKC – gender a věda.
Na semináři se dozvíte:
– jaké jsou požadavky evropských i domácích grantových agentur
– co je genderová dimenze a proč je důležité ji zohlednit
– jaká je souvislost s ins􀀃tucionální změnou a plány genderové rovnos􀀃
– v čem spočívají koncepty intersekcionality a inkluzivity
– a jak vše začlenit do obsahu výzkumu

Seminář Ludovica Antiga, M.Sc.
2025-02-12 15:00 - 16:00 | Posluchárna Milana Haška / Milan Hašek Auditorium
“PIP2-RNA-protein interactions in phase separation of Pol II transcription”
The RNA Polymerase II transcription is a multifaceted process crucial for gene expression in eukaryotic cells. Over the last decade, liquid-liquid phase separation (LLPS) was shown to be crucial in regulation of RNA pol II transcription. We have shown that nuclear phosphoinositides are pivotal for regulating biological processes and PIP2 has been shown to localize with RNA Pol II machinery and nascent RNA, linking it to the initiation step of transcription. Here, by using lipid-interacting RNA sequencing (LIPRNAseq) and confocal microscopy, we found a PIP2-binding AU-rich RNA motif and identified the long non-coding RNA HANR (lncHANR) in complex with PIP2 as a part of oncogenic super-enhancers in perinucleolar region of the nucleus.Furthermore, we used PIP2-pull down coupled with mass spectrometry to identify RNA-dependent PIP2 interactome associated with nuclear processes emphasizing the role of PIP2 in liquid-liquid phase separation of RNA Pol II transcription machinery.

Lecture „Sleep, synuclein and microbiome“
2025-02-17 15:00 - 16:00 | Kinosal, Vídeňská 1083, Praha

Seminář Jana Smolíková, Ph.D. - ZRUŠENO
2025-02-19 15:00 - 16:00 | Posluchárna Milana Haška / Milan Hašek Auditorium
“Analysis of a novel chicken Pax6 mutant”
Pax6 is known as the „master control gene“ for eye formation, playing a crucial role in the development of the lens, retina, and other ocular structures. It drives the formation of the eye field, initiates lens differentiation, and helps establish the retina—all vital for vision.
While the role of Pax6 in eye development is well-studied in other species, there has been little research on Pax6 in chickens specifically. This gap in knowledge presents an exciting opportunity to understand how the mutation of Pax6 impacts eye formation in birds.
In this seminar we’ll explore the role of the Pax6 transcription factor in chicken eye development and this could provide valuable insights into vertebrate eye development.

Seminář Iris Durić
2025-02-26 15:00 - 16:00 | Posluchárna Milana Haška / Milan Hašek Auditorium
“Transmembrane adaptor protein WBP1L: searching for its binding partners to understand its role in hematopoiesis”
Transmembrane adaptor protein WBP1L, also known as Outcome Predictor of Acute Leukemia 1 (OPAL1) was identified nearly 20 years ago as a positive prognostic marker in childhood acute lymphoblastic leukemia. More recently, elevated WBP1L expression has also been linked to positive outcomes in ovarian cancer. Despite these clinical associations, the physiological function of WBP1L has long remained unclear. Recently we demonstrated its involvement in the regulation of hematopoiesis, hematopoietic stem cell functionality and hematopoietic stem cell performance during transplantation. As an adaptor protein, WBP1L’s activity depends on its interacting partners. This presentation will describe our efforts to identify these proteins and understand their contributions to WBP1L function, focusing on two novel interactions: one with the receptor for interferon gamma and the other with the SCFβ-TRCP1 ubiquitin ligase complex.

Seminář Andrea Pauli
2025-03-05 15:00 - 16:00 | Posluchárna Milana Haška / Milan Hašek Auditorium
“Fundamental principles during the egg-to-embryo transition”
Fertilization initiates the egg-to-embryo transition, one of the most dramatic developmental transition resulting in the transformation of the egg from a dormant state into regulatorily and functionally distinct embryonic cells. While this transition has been studied extensively in respect to zygotic genome activation, the molecular mechanisms that mediate sperm-egg binding and fusion during fertilization and regulate the maintenance of dormancy in the egg and re-activation in the embryo remain poorly understood. The vision of the Pauli lab is to gain mechanistic insights into the egg-to-embryo transition, with a specific focus on the molecular control of fertilization and developmentally programmed dormancy and re-activation.
Andrea (Andi) Pauli will talk about recent findings from her lab related to their work towards uncovering the mechanism of vertebrate fertilization and translational regulation during the egg-to-embryo transition. By combining genetic, molecular, cellular, biochemical, structural and genomics approaches in their main model organism, the zebrafish, the long-term vision of the Pauli lab is to unravel new concepts and molecular mechanisms governing this fascinating developmental transition that marks the beginning of life.

Seminář Jakub Onhajzer, M.Sc.
2025-03-12 15:00 - 16:00 | Posluchárna Milana Haška / Milan Hašek Auditorium
“Reactivation of oncofetal genes reveals cellular heterogeneity and zonation in intestinal tumors”
Colorectal cancer is one of the most common malignancies. Recent studies have shown that tumor cells can reactivate so-called oncofetal genes — genes normally expressed during embryonic development but silenced in healthy adult intestinal tissue. These include Trop2 and Sca1, both of which are reactivated during the development and progression of intestinal tumors. Using a mouse model, we investigated the timing and molecular mechanisms driving the reactivation of these genes during the transformation of intestinal epithelial cells into tumor cells. By combining flow cytometry, 3D microscopy, and transcriptional profiling, we demonstrate that the re-expression of oncofetal genes is not uniform. Instead, it creates spatial heterogeneity — a phenomenon known as tumor zonation. This zonation is associated with the activation of fetal gene programs and the acquisition of stem cell-like properties. We also show that Sca1– and/or Trop2-positive tumor cells activate distinct molecular pathways and display unique biological properties. As several oncofetal genes (antigens) are already being explored as potential therapeutic targets, understanding the mechanisms underlying their reactivation could contribute to the development of new targeted therapies for colorectal cancer.

Seminář Jakub Sumbal
2025-03-19 15:00 - 16:00 | Posluchárna Milana Haška / Milan Hašek Auditorium
“Probing the functional heterogeneity and hierarchy in mammary gland fibroblasts”
In this talk, I will focus on the heterogeneity and hierarchy of mouse mammary fibroblasts – the predominant cell type of mammary stroma. By single cell RNA sequencing, genetic lineage tracing and microscopy we show that fibroblast transcriptomic clusters represent spatially differentiated populations with specific functions. Moreover, we used the lineage tracing to show differentiation traits within fibroblast populations and other stromal cell types. Finally, I will discuss how the regulation of fibroblast differentiation may be important for proper organ morphogenesis and cancer progression.

Přednáška pro nevědce a nevědkyně - V. Hořejší
2025-03-24 14:00 - 15:00 | Posluchárna Milana Haška / Milan Hašek Auditorium
Poučení z covidové epidemie – co dělat příště jinak
Covidová krize v letech 2020-2022 výrazně zasáhla do životů nás všech. Co se udělalo dobře a co naopak špatně? Jak obstáli občané, politici, vědci a zdravotníci? Jak je možné, že se americkým ministrem zdravotnictví stává výrazný antivaxer a šiřitel „fejků“? Pochází covidový virus z přírody nebo z laboratoře? Pomohou nám získané zkušenosti při příští epidemii, která jistě někdy přijde?
Zajímavých otázek je mnoho…

Lectures – Laboratory of Experimental Hypertension, IPHYS
2025-03-24 15:00 - 16:00 | Kinosal, Vídeňská 1083, Praha
Silvie Hojná: Anorexigenic neuropeptides in the treatment of obesity
Obesity and its complications have reached epidemic proportions. Therefore, finding new, more effective drugs for the treatment of obesity without side effects is a major challenge. Natural anorexigenic neuropeptides have this potential, but their major disadvantage is their low stability in plasma and inability to cross the blood-brain barrier (BBB) after peripheral administration. One strategy to develop peptidic drugs is based on peptide lipidization. This modification leads to increased stability and half-life of the peptide in the organism and allows its application in the periphery because lipidized peptides are able to cross the BBB. In Monday’s seminar, you will hear the full story of the development of anorexigenic analogs of prolactin-releasing peptide (PrRP), including their implications not only for the treatment of obesity, but also for their other potential applications.
Mahak Arora: Role of mTOR in cardiometabolic dysfunction
Cardiometabolic disorders (CMDs) such as hypertension, diabetes and obesity are associated with damage to key organs such as the heart, liver and kidneys. The mammalian target of rapamycin (mTOR) signaling pathway, which is critical for cell metabolism and growth, is often dysregulated in these diseases. In a mouse model of steatohepatitis, the mTOR inhibitor KU-0063794 ameliorated oxidative stress, reduced liver triglycerides and downregulated TNF-alpha mRNA by altering mTOR signaling. Recent pilot studies in high-fat fed SHR rats treated with empagliflozin and salt-sensitive Dahl rats exposed to high or low salt diet showed significant changes in mTOR-related genes such as mtor, akt, pgc1a, srebp, etc. in liver and kidney. These results suggest that mTOR plays a key role in the regulation of cardiometabolic functions

Seminář Jakub Gemperle, Ph.D.
2025-03-26 16:00 - 17:00 | Posluchárna Milana Haška / Milan Hašek Auditorium
“Live-cell magnetic micromanipulation of recycling endosomes reveals their direct effect on actin-based protrusions to promote invasive migration”
Endocytic recycling pathways play key roles in the re-routing of cargoes through the cell to control a broad range of cellular processes, and many vesicle trafficking regulators are implicated in progression of disease such as cancer. The Rab11 family (Rab11a, Rab11b, and Rab25) control return of internalised cargoes to the plasma membrane, and Rab25 has been implicated in the aggressiveness of cancer by promoting invasive migration. However, whilst Rab25 vesicles distribute to the leading of edge of moving cells, how directly they contribute to cell protrusion is not clear. Here we adopt a magnetogenetic approach that allows direct manipulation of Rab25 positioning to show that localisation to the cell periphery drives the formation of F-actin protrusions. We demonstrate that endogenous Rab25 vesicles coordinate the positioning of key cargoes, including the actin regulator FMNL1 and integrin β1, with the activation of Rho GTPases at the plasma membrane to generate and maintain F-actin rich filopodial protrusions and promote cancer cell invasive migration in 3D matrix.

Course Processing and Analysis of Microscopic Images in Biomedicine
2025-03-31 - 2025-04-04 | IMG
Dear Colleagues,
the Institute of Molecular Genetics of the Czech Academy of Sciences would like to invite you to the 5-day course „Processing and analysis of microscopic images in biomedicine (PAMIB25)“ intended for PhD students and junior researchers.
The course will address fundamental aspects of image data acquisition, processing, and analysis, encompassing techniques in stereology. Alongside theoretical principles, the course will prioritize hands-on practical learning. Participants will gain proficiency in utilizing the freely available software package Fiji for both basic and advanced analyses. They will learn to assess co-localizations, analyze data from FRAP and electron microscopy, track particles, segment objects in images, and explore methods of employing artificial intelligence for image segmentation.
Additionally, participants will master techniques to enhance data quality through deconvolution using Huygens software. An interactive session featuring Imaris software will be complemented by practical exercises. Furthermore, this year introduces a session utilizing NIS-Elements, a platform for image processing developed in Prague, and a lecture about the publishing images in scientific journals. Independent practical tasks involving Fiji and Huygens will also constitute a significant component of the course.
The course will be held at the Institute of Molecular Genetics of the Czech Academy of Sciences in Prague in March 31 – April 4, 2025. For more information and link to the registration form, please, visit the course website: https://course.img.cas.cz/pamib/. The registration deadline is March 17.
While the course aligns with the Microscopy Methods in Biomedicine, prior attendance is not a prerequisite for participation.
The course will be taught in English.
On behalf of the organizers,
Michaela Blažíková and Martin Čapek

20. Metodický seminář BIOCEV-Krč
2025-03-31 15:00 - 16:00 | Posluchárna Milana Haška / Milan Hašek Auditorium
„Etika a integrita vědeckého výzkumu a právní otázky“
Seminář se zaměří na témata: testy vědecké integrity; kdy zapojovat etické komise při řešení deliktů na pracovišti; význam závěrů etických komisí v právních přístupech; autorský zákon v praxi a z hlediska práva.
JUDr. Ján Matejka, Ph.D.
ředitel Ústavu státu a práva AV ČR, v. v. i.

Seminář Diego F. Joseph, M.Sc.
2025-04-02 15:00 - 16:00 | Posluchárna Milana Haška / Milan Hašek Auditorium
“Life Through Colors: Single-cell fluorescence-based reporter system for monitoring gene expression”
The presentation will depict a long journey from decision to actual making a novel reporter system for RNA silencing, which could also be adopted for studying other mechanisms manipulating gene expression. This development lead to production of two types of triple-color reporter HeLa lines where fluorescence reporters are inserted in frame in into endogenous genes encoding highly abundant soluble nuclear proteins. The system now allows for monitoring repression (loss of specific dluorescence) and derepression (appearence of previously silenced fluorescence) across the entire population of cultured cells and, possibly, for high throughput screening.

Euro-BioImaging Image Data Community Days 2025
2025-04-07 - 2025-04-11
Dear imaging users,
we would like to inform you about the Image Data Community Days that will be happening from 7-11. April this year.
This free week-long online event will bring together Euro-BioImaging Node staff, researchers and other experts from around the world to explore and exchange on the latest advances in image data through a mix of workshops, lectures and interactive discussions.
The Euro-BioImaging Data Team is pleased to announce that as of now we have officially opened registrations for this event! Find out all about the event and the preliminary agenda here:
https://www.eurobioimaging.eu/our-events/image-data-events/image-data-community-days/
We are very much looking forward to your participation in the week!
Best,
Euro-BioImaging Data Team

Seminář Jana Smolíková, Ph.D.
2025-04-09 15:00 - 16:00 | Posluchárna Milana Haška / Milan Hašek Auditorium

Seminář Goncalo Oliveira, Ph.D.
2025-04-16 15:00 - 16:00 | Posluchárna Milana Haška / Milan Hašek Auditorium
“Deciphering PNKP Functions in Neurological Disorders”
Hereditary mutations in polynucleotide kinase-phosphatase (PNKP), a key enzyme in DNA strand break repair, result in a broad spectrum of neurological disorders, ranging from mild neurodegeneration to severe neurodevelopmental defects. PNKP has dual phosphatase and kinase activities essential for DNA break repair, but their specific contributions to preventing neuropathology remains unclear. In this seminar, we will present findings from mouse genetic models that highlight the crucial role of the PNKP phosphatase domain in neurodevelopment. Additionally, we will introduce a novel cell-based system to investigate the immediate effects of acute PNKP depletion and explore how this model helps identify key sources of DNA damage and the molecular mechanisms underlying PNKP-related disease pathology.

Seminář Irena Adkins, Ph.D.
2025-04-23 15:00 - 16:00 | Posluchárna Milana Haška / Milan Hašek Auditorium
“Preclinical development of IL-15-based immunocytokine SOT201 and antibody-drug conjugate SOT106 for treatment of solid tumors”
SOT201 is a novel cis-acting immunocytokine consisting of an antibody against PD-1 genetically fused to a sushi+ domain of IL-15Ra and IL-15 mutein. SOT201 targets PD-1+ TILs with a balanced cytokine potency to revive exhausted T cells in tumor microenvironment. SOT201 is currently being evaluated in Phase I clinical study VICTORIA-01 (NCT06163391) in metastatic advanced cancer patients.SOT106 is a novel antibody-drug conjugate (ADC) targeting LRRC15, comprised of a proprietary humanized monoclonal antibody conjugated to monomethyl auristatin E (MMAE) via a beta-glucuronidase (GUSB) cleavable linker. SOT106 anti-tumor efficacy was confirmed in soft tissue sarcoma and pediatric osteosarcoma models with moderate to low LRRC15 expression supporting its clinical development as a novel therapy for sarcomas. Combined with its superior performance over a clinical benchmark ADC, these results underscore the potential of SOT106 as a best-in-class targeted treatment for these challenging malignancies.

Seminar: "The unfolded protein sensor IRE1 as a regulator of homeostatic dendritic cell maturation"
2025-04-28 10:00 - 11:00 | Posluchárna Milana Haška / Milan Hašek Auditorium
Dear friends and colleagues,
it is my pleasure to invite you to a special seminar given by

Prof. Sophie Janssens, PhD.
VIB-UGent Center for Inflammation Research, Belgium
Title: The unfolded protein sensor IRE1 as a regulator of homeostatic dendritic cell maturation

Monday, April 28, 2025 at 10 am, in Hasek’s lecture hall, Institute of Molecular Genetics (Videnska 1083)
Then, after the seminar you are invited to join us for an informal post-seminar hangout with the speaker, 🍕 A PIZZA TALK 🍕🎤 Open Q&A on science, careers, life in academia, women in science & more.👩‍🔬 For students & early-career scientists.🍕 Yes, there will be pizza.
Prof. Sofie Janssens’s research team focuses on the role of the unfolded protein response (UPR) in several immune cell types. Their recent work has shown that the UPR is an important component in the functioning of the immune system. In CD8α conventional dendritic cells (DCs) the UPR sensor IRE-1α and its downstream transcription factor XBP-1s is constitutively active and deficiency of XBP-1s leads to defects in cross-presentation of dead cell-derived antigens.
Examples of recent publications:
Striking a balance: new perspectives on homeostatic dendritic cell maturation.
Bosteels Victor, Janssens Sophie
NATURE REVIEWS. IMMUNOLOGY, 125-140, 25, 2025
DOI: 10.1038/s41577-024-01079-5
Paneth cell TNF signaling induces gut bacterial translocation and sepsis.
Wallaeys Charlotte, Garcia Gonzalez Natalia, Timmermans Steven, Vandewalle Jolien, Vanderhaeghen Tineke, De Beul Somara, Dufoor Hester, Eggermont Melanie, Moens Elise, Bosteels Victor Show more
CELL HOST & MICROBE, 1-19, 32, 2024
DOI: 10.1016/j.chom.2024.08.007
Activation of goblet-cell stress sensor IRE1ß is controlled by the mucin chaperone AGR2.
Cloots Eva, Guilbert Phaedra, Provost Mathias, Neidhardt Lisa, Van De Velde Evelien, Fayazpour Farzaneh, De Sutter Delphine, Savvides Savvas, Eyckerman Sven, Janssens Sophie
THE EMBO JOURNAL, 695-718, 43, 2024
DOI: 10.1038/s44318-023-00015-y
The IRE1ß-mediated unfolded protein response is repressed by the chaperone AGR2 in mucin producing cells.
Neidhardt Lisa, Cloots Eva, Friemel Natalie, Weiss Caroline A, Harding Heather, Mclaughlin Stephen, Janssens Sophie, Ron David
THE EMBO JOURNAL, 719-753, 43, 2024
DOI: 10.1038/s44318-023-00014-z
Canonical IRE1 function needed to sustain vigorous natural killer cell proliferation during viral infection.
Vetters Jessica, Van Helden Mary, De Nolf Clint, Rennen Sofie, Cloots Eva, Van De Velde Evelien, Fayazpour Farzaneh, Van Moorleghem Justine, Vanheerswynghels Manon, Vergote Karl Show more
ISCIENCE, 108570, 26, 2023
DOI: 10.1016/j.isci.2023.108570
LXR signaling controls homeostatic dendritic cell maturation.
Bosteels Victor, Marechal Sandra, De Nolf Clint, Rennen Sofie, Maelfait Jonathan, Tavernier Simon, Vetters Jessica, Van De Velde Evelien, Fayazpour Farzaneh, Deswarte Kim Show more
SCIENCE IMMUNOLOGY, eadd3955, 8, 2023
DOI: 10.1126/sciimmunol.add3955
— Dominik Filipp, PhD Laboratory of Immunobiology Institute of Molecular Genetics AS CR Videnska 1083 CZ-142 20 Prague 4 Czech Republic

Seminář Mohamed Elgendy, Ph.D.
2025-04-30 15:00 - 16:00 | Posluchárna Milana Haška / Milan Hašek Auditorium
„SHOULD CANCER PATIENTS EAT DIFFERENTLY? Metabolomics-guided dietary intervention for optimal clinical use of anti-cancer MCL1 inhibitors.”
Diet is the major source of nutrients for tumors. However, dietary interventions in cancer patients lack personalization. One of our active research areas is aimed at tailoring metabolomics-guided strategies to enhance the therapeutic effects of anti-cancer agents and/or ameliorate their toxicity. A new class of anti-cancer agents targeting Myeloid Cell Leukemia 1 (MCL1) protein is MCL1 is upregulated in around 10% of all tumors and contributes to tumorigenersis by allowing tumors to evade apoptosis. However, our recent findings (Gui et al., In revision in Nature Comm.) have identified unexpected functions for MCL1 in regulating cellular metabolism positioning MCL1 as a hinge linking the two hallmarks of cancer of “deregulated energetics” and “evading cell death”. Using these novel insights and exploiting state-of-the-art humanized Mcl-1 mice, we devised dietary approaches to optimize the clinical use of MCL1 inhibitors currently under clinical testing.

Seminář César Nombela-Arrieta
2025-05-07 15:00 - 16:00 | Posluchárna Milana Haška / Milan Hašek Auditorium
“Structural and functional remodeling of the bone marrow stromal microenv. during tissue regeneration and acute myeloid leukemia”
Bone marrow (BM) tissues are the prime sites for hematopoietic cell production, long-term reservoirs for immunological memory and hubs in which bone metabolism is orchestrated. The BM is comprised of a complex hematopoietic compartment, as well as a heterogeneous stromal fraction made of endothelial, mesenchymal and neural cells, which beyond proving the necessary tissue infrastructure, play fundamental regulatory roles in fine-tuning hematopoietic cell production. Stromal cells have also been shown to crucially contribute to hematological disease and marrow regeneration post-myeloablative damage. We combine 3D quantitative microscopy (3D-QM) techniques pioneered in our lab, novel reporter mouse models and single cell sequencing to analyze the compositional and structural organization of BM stroma in murine and human marrow samples. In this talk I will describe our findings on the dynamic spatial reorganization of major stromal components, and the localization of HSCs during the remodeling process that leads to the regeneration of normal marrow tissues following myeloablation. I will also present our latest work on the detailed single cell analysis of the human BM microenvironment and the alterations presented during aging as well as in patients with Acute Myeloid Leukemia.

4th 10x Genomics Experience Meeting 2025
2025-05-12 10:00 - 16:00 | Posluchárna Milana Haška / Milan Hašek Auditorium
Dear Colleagues,

We are pleased to invite you to the 4th 10x Genomics Experience Meeting, taking place on May 12, 2025, from 10:00 AM to 4:00 PM at IMG (Hašek's Auditorium).

Please find the detailed workshop program attached and join us for discussions on the current advancements in single-cell and spatial technologies.

To ensure your participation please register at https://10xgen.com/1Ls

We look forward to seeing you there!

Warm regards,

Michal Kolář

—
Laboratory of Genomics and Bioinformatics
Institute of Molecular Genetics of the Czech Academy of Sciences

AI-BASED SEGMENTATION AND TRACKING
2025-05-13 - 2025-05-14 | IMG
Dear all!
If you wish to analyze your microscopy image data efficiently using techniques of artificial intelligence,
YOU ARE INVITED to the practical course:
AI-BASED SEGMENTATION AND TRACKING
This intensive 2-day course takes place on May 13-14, 2025, at the Institute of Molecular Genetics of the Czech Academy of Sciences and focuses intensively on image data segmentation, cell tracking and denoising using state-of-the-art deep learning methods. Detailed information can be found at:
https://course.img.cas.cz/aist/
The deadline for registration is April 30.
Best regards, Martin Čapek, Michaela Blažíková, Jan Valečka and Pavel Krist (Zeiss)

Seminář Jacobus van Grootel, B.Sc.
2025-05-14 15:00 - 16:00 | Posluchárna Milana Haška / Milan Hašek Auditorium
“Evolutionary diversification of actomyosin-driven early embryo morphogenesis”
The shape of an animal arises during embryo morphogenesis and involves rotatory movements of
cells relative to eachother. These movements are driven by forces that arise in the actomyosin
cytoskeleton. Biophysical studies in C. elegans have unraveled many of the physcial mechanisms
underlying morphogenetic cell rotations. However, little is known about whether and how these
physical mechanisms diversified. We set out to study this by analyzing actomyosin-driven
morphogenesis in a panel of nematode species that covers more than 100 million years of
evolutionary distance. Although the overall pattern of cell orientations displays little variability in
these nematodes, we find striking differences in the actomyosin behavior. This indicates that
different force-generating mechanisms underlie similar types of morphogenetic cell rotations.
Altogether, these results are indicative of developmental systems drift, where different physical
mechanisms give rise to a similar developmental outcome.

Seminář David Machač, M.Sc.
2025-05-21 15:00 - 16:00 | Posluchárna Milana Haška / Milan Hašek Auditorium
“RORγt⁺ Regulatory T Cells establish a memory footprint for the primary microbiota”
A balanced immune system relies on regulatory T cells (Tregs) to prevent excessive immune responses. While thymus-derived Tregs are well known, a unique subset of microbiota-specific, peripherally induced RORγt⁺ Tregs has emerged as a critical regulator of intestinal immunity and crosstalk with microbial communities. Our data suggests that RORγt⁺ Tregs function as an immunological “memory,” safeguarding beneficial primordial microbiota, thus offering a novel paradigm for gut immune homeostasis.

Lectures „Thermoneutrality and thermogenesis“ and „Thermogenesis: by which means?“
2025-05-26 15:00 - 16:00 | Kinosal, Vídeňská 1083, Praha

Seminář Alexandra Urbančoková, M.Sc.
2025-05-28 15:00 - 16:00 | Posluchárna Milana Haška / Milan Hašek Auditorium
“PML nucleolar association: What is it and what does it do?”
Promyelocytic leukemia protein (PML) is a multivalent stress response protein that, upon specific genotoxic stress, can organize into a unique structure that encloses nucleolar material, termed the PML-nucleolar association (PNA). PNAs were described in both senescent cells and cells harboring DNA damage. We investigated the origin, dynamics, and signaling pathways leading to PNA formation. Our recent findings suggest that PNAs are involved in ribosomal DNA (rDNA) damage response by sequestration of persistent rDNA lesions and promote cellular senescence. Further analysis of PNA-associated proteins reinforces their involvement in the rDNA damage response. Together, these insights contribute to a more comprehensive understanding of the functional relevance of PNAs in rDNA maintenance and cellular genotoxic stress response.

Přednáška pro nevědce a nevědkyně - V. Varga
2025-06-02 15:00 - 16:00 | Posluchárna Milana Haška / Milan Hašek Auditorium
„Cesta do nitra buňky“
Během přednášky podnikneme cestu do nitra živé buňky. Ukážeme si, jaké nástroje vědci používají, aby odhalili její mikroskopické struktury, a jak lze tyto detaily převést do podoby, kterou můžeme spatřit
pouhým okem. Zároveň zjistíme něco překvapivého – naše buňky mají víc společného, než byste čekali, s buňkami nebezpečných parazitů, například s původcem spavé nemoci. Jak moc jsme si podobní a co to pro nás znamená? To vše odhalíme v průběhu přednášky.
