Seminář Prasoon Kumar Thakur, M.Sc.
2024-02-28 15:00 - 16:00 (Europe/Prague) | Posluchárna Milana Haška / Milan Hašek Auditorium
“Splicing in the world of marine diplonemids”
Recent surveys of marine biodiversity have revealed the abundance and diversity of planktonic diplonemids. By sequencing the genomes and transcriptomes of six marine diplonemids and performing comprehensive splice site analyses, we identified numerous non-canonical introns and mapped their common features, shedding light on the evolution of RNA splicing in these abundant unicellular organisms.

IEM CAS μLectures: Microscope objectives
2024-02-27 13:00 - 14:00
μLectures is a new, monthly seminar series designed to enhance researchers’ understanding of the fundamental workings and principles of microscopy. Each session, lasting 30 minutes, will focus on a specific concept or topic within microscopy. The practical information presented aims to deepen researchers’ comprehension of their microscopy experiments, giving them better overview of the options available and enabling them to improve their experimental design, and more effectively identify and resolve potential issues.
Annotation
The first session will focus on objectives. It will introduce different types of objectives and their relevant parameters, explore the interaction between immersion and medium/fixation methods, and discuss how objective selection can impact image quality.
The seminar will take place on 27. 2. 2024 from 1 p.m. in the Turquoise Auditorium of the IEM CAS. It will also be possible to join online using this link (opens in new window).
It will be held in English.
Registration
Registration is not required, but if you are interested, you can let us know (in person or on iva.svecova@iem.cas.cz) to give us an idea about how many people are planning to come.
Lecturer
Iva Švecová

Den fyziologie a medicíny pro vysokoškoláky
2024-02-22 09:30 - 15:00 | Kinosal, Vídeňská 1083, Praha
Studujete vysokou školu přírodovědeckého nebo medicínského zaměření? Připravili jsme pro vás speciální den otevřených dveří s nabídkou zajímavých témat pro vaši bakalářskou či magisterskou práci i témata pro studium PhD. Vědci z FGÚ vás s nimi seznámí v dopoledním bloku (prezenční i online formou) a odpoledne budete mít možnost nahlédnout přímo do jejich laboratoří. Celý program je v angličtině.
Přihlašování a program: https://www.fgu.cas.cz/articles/1114-program-den-fyziologie-a-mediciny-pro-vysokoskolaky-2024

Seminář Ing. Eliška Gáliková
2024-02-21 15:00 - 16:00 | Posluchárna Milana Haška / Milan Hašek Auditorium
“Knocking out spo11 in chicken arrests meiosis but does not prevent further development of germ cells”
Knockout of the mouse spo11 prevents double-strand breaks and formation of synaptonemal complex during meiosis. As a result, the development of spermatocytes is arrested at zygotene and the arrested cells undergo apoptosis. Not so in the chicken. Here, although we also observe arrest of meiosis and impaired fertility, diploid spermatocytes and oocytes continue to develop with interesting biological consequences.

Hodnocení výzkumu v Akademii věd ČR
2024-02-20 14:00 - 15:00 | Posluchárna Milana Haška / Milan Hašek Auditorium
Přednášející: prof. Ing. Jiří Homola, CSc., DSc.
Organizace přednášky byla projednána a schválena na schůzi Rady areálu dne 15.1.2024.
Vzhledem k projednávanému tématu a v návaznosti na administraci procesu hodnocení, se přednáška bude konat pouze v českém jazyce.

Lecture "Microtubule associated proteins and their regulation - between brain development and disease"
2024-02-19 15:00 - 16:00 | Kinosal, Vídeňská 1083, Praha
Martin Balaštík, Ph.D., Laboratory of Molecular Neurobiology, IPHYS

Seminář Miroslav Stoyanov, M.Sc.
2024-02-14 15:00 - 16:00 | Posluchárna Milana Haška / Milan Hašek Auditorium
“PPM1D activity promotes genome instability and cellular transformation”
Protein phosphatase magnesium dependent 1 (PPM1D) is a negative regulator of the tumour suppressor p53 and is implicated in termination of DNA damage response. Increased PPM1D activity allows proliferation in the presence of DNA breaks caused by ionizing radiation or replication stress induced by expression of active RAS oncogene. Active PPM1D promotes cell growth in the soft agar and formation of tumours in nude mice confirming the oncogenic potential of PPM1D.

Seminář Dr. rer. nat. Leoš Shivaya Valášek, DSc.
2024-02-07 15:00 - 16:00 | Posluchárna Milana Haška / Milan Hašek Auditorium
“Ribosomal A-site interactions with near-cognate tRNAs drive stop codon readthrough”
Imagine a gene is a sentence starting with a capital letter and ending with a period, and a genome as a book telling an entire story. In some protozoans, extra periods infiltrated their sentences, replacing specific letters of arbitrary words (namely E and W). As a r.sult, r.aders (ribosom.s) are confus.d about .here these sentenc.s really .nd, and the story b.comes disjoint.d. We described how these protozoans handled this mystery, shedding a new light on (non)universality of the genetic code.

IEM CAS Institutional Seminar Series 02/2024
2024-02-01 14:00 - 15:00
The role of miR-20a in the injured CNS
Lecturer: Mgr. Ivan Arzhanov / Department of Neuroregeneration
Annotation: Spinal cord injury (SCI) involves damage to any part of the spinal cord, leading to nervous cell death and disruption of conduction pathways. This damage results in the loss of motor and sensory function below the injury site, potentially causing paralysis and, in severe cases, complete paralysis. Following SCI, a myriad of genes undergo dysregulation, accompanied by alterations in the expression of microRNAs (miRNAs). MiRNAs, small non-coding RNA molecules, play a pivotal regulatory role in various biological processes by interfering with mRNA. In our experiments, the expression of miR-20a was significantly upregulated for at least one week post-trauma. MiR-20a targets numerous genes, hindering the regenerative process of nervous tissue. However, inhibiting miR-20a decreases pro-apoptotic gene expression while facilitating the expression of anti-apoptotic genes, thereby diminishing neural cell death after injury. During this seminar, we will summarise the data regarding changes in mRNAs, miRNAs, and protein expression following SCI. Subsequently, we will specifically focus on miR-20a and its role in basic cellular processes following SCI.
The seminar will take place on 1. 2. 2024 from 2 p.m. in the Turquoise Auditorium of the IEM CAS.
We look forward to your participation.

Seminář Maria Kuzmina, M.Sc.
2024-01-31 15:00 - 16:00 | Posluchárna Milana Haška / Milan Hašek Auditorium
“HSCs in inflammation: go extramedullary or go home”
Hematopoietic stem cells (HSCs) are a rare cell type residing in the bone marrow and giving rise to all hematopoietic cells. Despite their quiescence, HSCs respond to extracellular signals which regulate their fate. In this presentation, we will determine how of chronic inflammation affects HSC location and function.

BioCEV-Krč Methodological Seminar
2024-01-29 15:00 - 16:00 | Kinosal, Vídeňská 1083, Praha
Topic: Spatial metabolomics
Program: https://www.fgu.cas.cz/significant/event/495

Seminář Martin Estermann, Ph.D.
2024-01-24 15:00 - 16:00 | Posluchárna Milana Haška / Milan Hašek Auditorium
“The cell biology and molecular genetics of avian gonadal development”
I am interested in investigating the regulatory mechanisms governing sex differentiation in avian species, with a specific focus on utilizing the chicken as a model organism. In the course of my presentation, I will explore the cellular foundations and molecular components dictating the development of ovarian and testicular fate in avian species. Employing comparative approaches, I will scrutinize the distinctive attributes of the ZZ and ZW chromosome sex determination system, elucidating its role in cell-autonomous sex determination. Lastly, I will share my recent postdoctoral findings particularly the metabolic regulation of sex differentiation.

IEM CAS Institutional Seminar Series (replacement date from 10/2023)
2024-01-18 14:00 - 15:00
Lsp1 partially substitutes Pil1 function in eisosome assembly under stress conditions
Lecturer: Ing. Petra Veselá, Ph.D. / Department of Functional Organisation of Biomembranes
Annotation: Eisosomes are large hemitubular structures that underlie the invaginated microdomains in the plasma membrane of various organisms and take part in the cellular stress response. In the yeast Saccharomyces cerevisiae, they are organised by BAR-domain containing proteins Pil1 and Lsp1. While under normal laboratory conditions, deletion of the PIL1 gene results in the inability of cells to assemble eisosomes, deletion of LSP1 shows no apparent phenotype. We show that under stress conditions, Lsp1 takes over the Pil1 function and forms eisosomes even in the absence of Pil1.
The seminar will take place on 18. 1. 2024 from 2 p.m. in the Turquoise Auditorium of the IEM CAS.
We look forward to your participation.

Seminář Viktor Bugajev, Ph.D.
2024-01-17 15:00 - 16:00 | Posluchárna Milana Haška / Milan Hašek Auditorium
“The novel sphingolipid synthesis regulators in cell life”
Sphingolipids are bioactive lipids and critical structural components of cell membranes. Regulation of their de novo synthesis is critical for normal mammalian development and physiology. Although the ORMDL family has been identified as the gatekeeper of the serine palmitoyl transferase (SPT) activity, our new data suggest that other components are also involved. We will present our results on new regulators of the SPT complex based on their interaction with members of the ORMDL family using genetic, immunogenetic and mass spectrometry approaches. Our data indicate that SPT regulatory circuits are more complex than previously thought.

IEM CAS Institutional Seminar Series 01/2024
2024-01-04 14:00 - 15:00
Improvement of healing of bone defects by new approaches of regenerative medicine
Lecturer: Mgr. Eva Šebová / Department of Tissue Engineering
Annotation: The PLA and PCL block copolymer combined with hydroxyapatite nanoparticles is a promising material for bone tissue engineering. We expect a high ability to seamlessly interact with bone cells and promote bone growth, which is at the core of our research. By exploring electrospun nanofibers, we aim to uncover the potential of copolymer with hydroxyapatite in new bone healing strategies, offering promising avenues for more effective and efficient bone regeneration therapies.
The seminar will take place on 4. 1. 2024 from 2 p.m. in the Turquoise Auditorium of the IEM CAS.
We look forward to your participation.

Seminář - Miles Joseph Raishbrook, M.Sc.
2023-12-20 15:00 - 16:00 | Posluchárna Milana Haška / Milan Hašek Auditorium
„The impact of FAM84B in retinal homeostasis“
Fam84b is an incompletely characterised protein. Its aberrant expression has been associated with various human cancers, with a possible reported role in endosomal trafficking. We genetically ablated Fam84b expression in mice, and extensively characterised the subsequent age-related, degenerative phenotype in the retinas of knockout animals. The aim of the project is to explain how the lack of Fam84b unsettles retinal homeostasis and induces the presented phenotype.

Seminář - Valeria Buccheri, M.Sc.
2023-12-13 15:00 - 16:00 | Posluchárna Milana Haška / Milan Hašek Auditorium
“Canonical RNAi in vivo in mice”
Canonical RNAi, i.e. long dsRNA-induced mRNA degradation, is essentially not operating in vivo in mammalian somatic cells. Low efficiency of dsRNA conversion into small interfering RNA by Dicer was identified as key limiting factor for canonical RNAi in mammals. We have genetically engineered mice to express a more active Dicer variant and investigated how this impacts mouse development and RNAi activity in vivo.

IEM CAS Institutional Seminar Series 12/2023
2023-12-07 14:00 - 15:00
Sensorineural hearing loss in mice lacking GABAB receptor-associated KCTD12 protein
Lecturer: Mgr. Adolf Melichar / Department of Auditory Neuroscience
Annotation: KCTD12 protein is the auxiliary subunit of the G-protein coupled receptor for γ-aminobutyric acid (GABA-B). KCTD12 constitutively binds to the intracellular portion of GABA-B receptor and significantly modulates its structural and functional properties. KCTD12/GABA-B complexes are characterized by high expression levels in the mammalian peripheral and central auditory system, suggesting their important role in auditory function. In this presentation, we summarize our results showing that deletion of the KCTD12 gene in mice leads to hearing loss and behavioral manifestations of hyperacusis and tinnitus.
The seminar will take place on 7. 12. 2023 from 2 p.m. in the Turquoise Auditorium of the IEM CAS.
We look forward to your participation.

Seminář - Mgr. Luděk Štěpánek, Ph.D.
2023-12-06 15:00 - 16:00 | Posluchárna Milana Haška / Milan Hašek Auditorium
„Studying the eukaryotic cilium by a single organelle approach“
Cilia are present on the surface of many eukaryotic cells and have critical motility, sensory, and signalling functions. Yet, many fundamental aspects of their biology are poorly understood. Taking advantage of the fact, that the cilia are experimentally well accessible, I have been developing approaches for imaging and manipulation of individual cilia. These include correlative light and electron microscopy, blocking transport within the cilia by physical and chemical means, as well as in vitro reconstitution approaches. In this talk, I will discuss the development of such methods and present the advancements in our understanding of processes responsible for building and maintenance of the cilia these approaches have enabled.

IEM CAS Mini-Symposium BIOCEV Regeneration III.
2023-11-23 - 2023-11-24
Dear Colleagues,
We are pleased to announce the annual Mini-Symposium BIOCEV Regeneration III, which will be held on 23-24th November 2023 in BIOCEV, Vestec.
Our symposium aims to exchange knowledge in regenerative medicine, tissue engineering, 3D in vitro modelling, organoid research, new tools and technologies in cell culture, and many more.
Along with several lectures given by well-known experts in the fields, this year, we encourage early career researchers to contribute to our symposium and share research achievements, exchange opinions, or find new contacts and cooperations. To achieve the best welcoming atmosphere during the event, our organising team comprises PhD students of Charles University working in the Institute of Experimental Medicine of the CAS, v. v. i. and the Institute of Physiology of the CAS, v. v. i.
In a student-to-student format, we would like to invite our colleagues (PhD students, early post-doctoral scientists) to submit the abstracts and share their research via an oral presentation or a poster combined with a short pitch talk. Additionally, the active participants will benefit from a specially organised Student Workshop on the 24th of November, where our colleagues from research organisations and companies will share their knowledge in 3D culture, tissue engineering tools and methods, advise on the commercialisation of the research or the translation of the results into clinical applications. The Symposium will be held in English.
The main topics of the mini-symposium:

Tissue engineering
Regenerative medicine
In vitro models
3D cultures
Organoids
New tools and technologies in regenerative medicine and tissue engineering

Registration for the event is free of charge. To register as a delegate as well as an active participant with the abstract submission, please fill out the registration form here:

Registration and abstract submission: https://forms.gle/YhLqptsLwnzYuTTj8 (open in a new window)
The abstract submission deadline is 24.10. 2023.
The registration deadline is 10.11.2023

We are looking forward to seeing you in November!
Sincerely, Organising team

Lecturers and Committee Members

Pre-confirmed invited lecturers

Natalia Rozwadowska, PhD (The Institute of Human Genetics of the PAS, Poznan, Poland)
Dasa Bohaciaková, PhD (Masaryk University, Brno, Czech Republic)
Oleg Lunov, PhD (Institute of Physics of the CAS, Prague, Czech Republic)
Tomasz Kolanowski, PhD (The Institute of Human Genetics of the PAS, Poznan, Poland)
Lukas Valihrach, PhD (Institute of Biotechnology of the CAS, BIOCEV, Vestec, Czech Republic)

Organising committee

Jarmila Havelkova, MSc (Institute of Physiology of the CAS, v. v. i. & Institute of Experimental Medicine of the CAS, v. v. i., BIOCEV, Charles University)
Kristyna Sintakova, MSc (Institute of Experimental Medicine of the CAS, v. v. i., BIOCEV, Charles University)
Eliska Vavrinova, MSc (Institute of Experimental Medicine of the CAS, v. v. i., BIOCEV, Charles University)

Scientific committee

Assoc. Prof. Lucie Bacakova, MD, PhD (Institute of Physiology of the CAS, v. v. i.)
Assoc. Prof. Pavla Jendelova, PhD (Institute of Experimental Medicine of the CAS, v. v. i.)
Yuriy Petrenko, PhD (Institute of Experimental Medicine of the CAS, v. v. i. & Institute of Physiology of the CAS, v. v. i., BIOCEV)
Olena Rohulska, PhD (Institute of Experimental Medicine of the CAS, v. v. i. & Institute of Physiology of the CAS, v. v. i., BIOCEV)
Nataliya Romanyuk, PhD (Institute of Experimental Medicine of the CAS, v. v. i.)

Our sponsors

East Port Life Science
Elmarco
Schoeller Instruments
SVEN Biolabs
TATAA Biocenter

Seminář - RNDr. Ondřej Mihola, Ph.D.
2023-11-22 15:00 - 16:00 | Posluchárna Milana Haška / Milan Hašek Auditorium
“A chance for fertility despite bad DNA breaks (AKA The effect of crossing-over rate on fertility)”
Programmed meiotic DNA breaks are essential for mammalian fertility but dangerous for genome integrity. DNA-binding H3-lysine-trimethyltransferase PRDM9 determines the positions of the meiotic DNA breaks, which can be repaired as crossovers or noncrossovers. The crossover/noncrossover decision is regulated by many factors that include RING-domain E3 ligase RNF212. The dosage of the Rnf212 gene in mouse affects the crossover rate per meiotic cell and variants in the human RNF212 gene are associated with crossover rates. Deficiency for RNF212 leads to complete arrest of meiosis at the metaphase stage. The deficiency for human and rodent PRDM9 causes partial to complete pachytene arrest, depending on unknown genetic factors. We will present results demonstrating that the dosage of Rnf212 (and thus crossover rate) affects meiotic progression and germ cell counts of semisterile Prdm9-deficient mice. Our findings offer an insight into the mechanisms and treatment possibilities of some cases of infertility.

Lecture
2023-11-20 15:00 - 16:00
Laboratory of Developmental Epileptology, IPHYS

IEM CAS Distinguished Guest Seminar Series - Prof. Jens Pahnke
2023-11-20 14:30 - 16:00
ABC transporter blood-brain barrier function in neurodegenerative and neuroinflammatory diseases – from basic research to treatment
Lecturer: Prof. Jens Pahnke, M.D., Ph.D., E.F.N./ University of Oslo, Faculty of Medicine / Oslo University Hospital, Department of Pathology, Norway
The seminar will take place on 20. 11. 2023 from 2:30 p.m. in the Turquoise Auditorium of the IEM CAS.
Annotation:
The current understanding of neurodegenerative diseases focusses on the deposition of toxic peptide species as larger aggregates in the brain of effected individuals. However, patients with sporadic neurodegenerative diseases do not overexpress these peptides. Moreover, they are hampered in removing the physiologically produced peptides, e.g. Aβ and α-synuclein. The blood-brain barrier plays a prominent role in the active and effective removal of metabolites and in the efflux of toxic peptides from the brain into the blood stream.
Here, ABC transporters were discovered by us and others to have a fundamental role in the homeostasis of the brain’s environment. In 2011, we described a new ABC transporter (ABCC1) that increases Aβ in the brain of knock-out animals by 12-14fold. Since then, we could show that the activation of ABC transporters can be used as a diagnostic tool and a new treatment option in elderly with Alzheimer’s disease and other neurodegenerative diseases, such as Huntington’s disease.
1 Pahnke, J. et al. Free Neuropathol 2, 33, (2021)
2 Pahnke, J. et al. Neurobiol Dis 72 Pt A, 54-60, (2014)
3 Pahnke, J. et al. Curr Alzheimer Res 5, 396-405, (2008)
We look forward to your participation.

Seminář - Prof. Jiří Forejt, M.D., Ph.D., DSc.
2023-11-15 15:00 - 16:00 | Posluchárna Milana Haška / Milan Hašek Auditorium
“Genetic architecture of Prdm9-driven reproductive isolation between mouse subspecies”
Hybrid incompatibility, the inviability or infertility of species hybrids, has played a central role in speciation research since Darwin’s Origin of Species. However, the search for genes controlling hybrid sterility, mainly using Drosophila species hybrids, has been largely unsuccessful, revealing a polygenic control with complex epistatic interactions. Fifty years ago, we identified the first hybrid sterility locus, Hst1, in the house mouse genome, and 14 years ago, we positionally cloned Prdm9 as the gene responsible for Hst1-controlled hybrid sterility.
In this talk, I will summarize our current understanding of the molecular genetic mechanisms that control this unique model of mammalian hybrid sterility.

Den otevřených dveří ÚMG / IMG Open House Day
2023-11-14 09:00 - 15:00

Lecture "Gliflozins in the treatment of non-diabetic cardiovascular diseases"
2023-11-13 15:00 - 16:00 | Kinosal, Vídeňská 1083, Praha
RNDr. Ivana Vaněčková, DSc., Laboratory of Experimental Hypertension, IPHYS

IEM CAS Open House Day / Week of the Czech Academy of Sciences Festival
2023-11-10
Week of the Czech Academy of Sciences is a science festival that follows the previous Week of Science and Technology of the CAS. The festival offers scientific lectures, exhibitions, events at institutes, documentary films, workshops and many other activities across the Czech Republic and all scientific disciplines. As part of the festival, the IEM CAS traditionally organises an Open House Day, which includes individual and group excursions around the research departments, as well as a cycle of lectures which presents the latest discoveries and achievements of the IEM CAS to the public. The morning programme is focused primarily on high school students.
Registration

Den otevřených dveří - FGÚ
2023-11-09 | Kinosal, Vídeňská 1083, Praha
Začátek v 9,00 hod a ve 13,00 hod.

Seminář – Assoc. Prof. Pavel Plevka
2023-11-08 15:00 - 16:00 | Posluchárna Milana Haška / Milan Hašek Auditorium

Lecture "Clocks in our body – how do they synchronize to shape our physiology?"
2023-11-06 15:00 - 16:00 | Kinosal, Vídeňská 1083, Praha
Prof. PharmDr. Alena Sumová, DSc., Laboratory of Biological Rhythms, IPHYS
