Seminář Miroslav Stoyanov, M.Sc.
2024-02-14 15:00 - 16:00 (Europe/Prague) | Posluchárna Milana Haška / Milan Hašek Auditorium
“PPM1D activity promotes genome instability and cellular transformation”
Protein phosphatase magnesium dependent 1 (PPM1D) is a negative regulator of the tumour suppressor p53 and is implicated in termination of DNA damage response. Increased PPM1D activity allows proliferation in the presence of DNA breaks caused by ionizing radiation or replication stress induced by expression of active RAS oncogene. Active PPM1D promotes cell growth in the soft agar and formation of tumours in nude mice confirming the oncogenic potential of PPM1D.

Lecture "Microtubule associated proteins and their regulation - between brain development and disease"
2024-02-19 15:00 - 16:00 | Kinosal, Vídeňská 1083, Praha
Martin Balaštík, Ph.D., Laboratory of Molecular Neurobiology, IPHYS

Hodnocení výzkumu v Akademii věd ČR
2024-02-20 14:00 - 15:00 | Posluchárna Milana Haška / Milan Hašek Auditorium
Přednášející: prof. Ing. Jiří Homola, CSc., DSc.
Organizace přednášky byla projednána a schválena na schůzi Rady areálu dne 15.1.2024.
Vzhledem k projednávanému tématu a v návaznosti na administraci procesu hodnocení, se přednáška bude konat pouze v českém jazyce.

Seminář Ing. Eliška Gáliková
2024-02-21 15:00 - 16:00 | Posluchárna Milana Haška / Milan Hašek Auditorium
“Knocking out spo11 in chicken arrests meiosis but does not prevent further development of germ cells”
Knockout of the mouse spo11 prevents double-strand breaks and formation of synaptonemal complex during meiosis. As a result, the development of spermatocytes is arrested at zygotene and the arrested cells undergo apoptosis. Not so in the chicken. Here, although we also observe arrest of meiosis and impaired fertility, diploid spermatocytes and oocytes continue to develop with interesting biological consequences.

Den fyziologie a medicíny pro vysokoškoláky
2024-02-22 09:30 - 15:00 | Kinosal, Vídeňská 1083, Praha
Studujete vysokou školu přírodovědeckého nebo medicínského zaměření? Připravili jsme pro vás speciální den otevřených dveří s nabídkou zajímavých témat pro vaši bakalářskou či magisterskou práci i témata pro studium PhD. Vědci z FGÚ vás s nimi seznámí v dopoledním bloku (prezenční i online formou) a odpoledne budete mít možnost nahlédnout přímo do jejich laboratoří. Celý program je v angličtině.
Přihlašování a program: https://www.fgu.cas.cz/articles/1114-program-den-fyziologie-a-mediciny-pro-vysokoskolaky-2024

IEM CAS μLectures: Microscope objectives
2024-02-27 13:00 - 14:00
μLectures is a new, monthly seminar series designed to enhance researchers’ understanding of the fundamental workings and principles of microscopy. Each session, lasting 30 minutes, will focus on a specific concept or topic within microscopy. The practical information presented aims to deepen researchers’ comprehension of their microscopy experiments, giving them better overview of the options available and enabling them to improve their experimental design, and more effectively identify and resolve potential issues.
Annotation
The first session will focus on objectives. It will introduce different types of objectives and their relevant parameters, explore the interaction between immersion and medium/fixation methods, and discuss how objective selection can impact image quality.
The seminar will take place on 27. 2. 2024 from 1 p.m. in the Turquoise Auditorium of the IEM CAS. It will also be possible to join online using this link (opens in new window).
It will be held in English.
Registration
Registration is not required, but if you are interested, you can let us know (in person or on iva.svecova@iem.cas.cz) to give us an idea about how many people are planning to come.
Lecturer
Iva Švecová

Seminář Prasoon Kumar Thakur, M.Sc.
2024-02-28 15:00 - 16:00 | Posluchárna Milana Haška / Milan Hašek Auditorium
“Splicing in the world of marine diplonemids”
Recent surveys of marine biodiversity have revealed the abundance and diversity of planktonic diplonemids. By sequencing the genomes and transcriptomes of six marine diplonemids and performing comprehensive splice site analyses, we identified numerous non-canonical introns and mapped their common features, shedding light on the evolution of RNA splicing in these abundant unicellular organisms.

Lecture "AMPA glutamate receptor signaling – auxiliary subunits and beyond"
2024-03-04 15:00 - 16:00 | Kinosal, Vídeňská 1083, Praha
Dr. Ingo Greger, Neurobiology Division MRC Laboratory of Molecular Biology, Cambridge, UK

Seminář Simona Mikula Mršťáková, M.Sc.
2024-03-06 15:00 - 16:00 | Posluchárna Milana Haška / Milan Hašek Auditorium
“Role of pax6 genes in the eye during embryonal development of medaka”
Pax6 is remarkably conserved across a wide range of species, from flies to humans, signifying its fundamental role in the regulatory network governing eye formation. In this presentation, we will delve into the functions of three pax6 genes – Pax6.1, Pax6.2, and Pax6.3 – and their specific contributions to eye development during the embryonic stages of the Japanese medaka fish (Oryzias latipes). We will explore how these genes orchestrate the complex process of eye formation, underscoring their importance in developmental biology and evolutionary studies.

IEM CAS Institutional Seminar Series 03/2024
2024-03-07 14:00 - 15:00
Subpopulations of NG2 glia in the adult mouse brain after focal cerebral ischemia.
Lecturer: Mgr. Tomáš Knotek / Department of Cellular Neurophysiology
Annotation: Glia expressing NG2 antigen are known predominantly as precursors of oligodendrocytes. However, their differentiation potential in tissue affected by ischemic injury has not yet been fully explored. To further our understanding of these cells, we employed single-cell RNA sequencing, immunohistochemical staining, RT-qPCR, and patch-clamp technique on NG2-expressing cells in healthy tissue and in tissue affected by focal cerebral ischemia (FCI). Single-cell RNA sequencing data revealed that NG2 glia form distinct subpopulations, some of which can be ascribed to the proliferation of NG2 glia and their progression in the oligodendrocyte lineage. However, we also observed previously described astrocyte-like NG2 glia that appeared both in healthy and ischemic tissue. Interestingly, this subpopulation also shared properties with neural progenitor cells. Expression of genes linked to neurogenesis was confirmed by immunohistochemical staining and changed at different time points after FCI. Of note, 28 days after the stroke, we observed NG2-expressing cells positive for NeuN. Together, these results provide new insight into the plasticity of NG2 glia following ischemic injury.
The seminar will take place on 7. 3. 2024 from 2 p.m. in the Turquoise Auditorium of the IEM CAS.
We look forward to your participation.

Seminář Imtissal Krayem, Ph.D.
2024-03-13 15:00 - 16:00 | Posluchárna Milana Haška / Milan Hašek Auditorium
“Transmembrane adaptor protein OPAL1 regulates leukocyte hematopoiesis and thymus development”
Expression of transmembrane adaptor protein Outcome predictor of acute leukemia 1 (OPAL1), has been linked to the ETV6-RUNX1 translocation and favorable prognosis in childhood leukemia. Our research also suggests that it has an important role in leukocyte development. We show that in mice with germline deletion of OPAL1, hematopoiesis is dysregulated early on, affecting mainly hematopoietic stem cells and early progenitors. Dysregulations in this compartment also contribute to more efficient engraftment of OPAL1-deficient cells following hematopoietic stem and progenitor cell transplantations. In addition, the thymi of OPAL1-deficient mice are enlarged, housing increased numbers of thymocytes across all subsets. This phenomenon may be attributed to the elevated generation of MPP4/LMPP progenitors in the bone marrow, which subsequently give rise to thymus-seeding progenitors. In summary, WBP1L emerges as a key player in leukocyte homeostasis, maintaining leukocyte numbers during steady-state conditions and after bone marrow transplantation.

Seminář Zuzana Outlá, M.Sc.
2024-03-20 15:00 - 16:00 | Posluchárna Milana Haška / Milan Hašek Auditorium
„Targeting plectin-mediated cytoskeletal crosstalk in hepatocellular carcinoma“
Anotace: The study’s findings indicate that the elevation of plectin in tumor hepatocytes is associated with the progression of hepatocellular carcinoma (HCC). This progression can be inhibited through genetic or pharmacological targeting of plectin. The oncogenic and invasive potentials of HCC cells are attenuated through plectin ablation, suggesting that plectin contributes to HCC’s metastatic capacity. These results identify plectin as a potential therapeutic target in HCC.

Lecture "Hemodynamic mechanisms initiating salt-sensitive hypertension: Prevention options"
2024-03-25 15:00 - 16:00 | Kinosal, Vídeňská 1083, Praha
Ing. Michal Pravenec, DrSc., Laboratory of Genetics of Model Diseases, IPHYS

IEM CAS μLectures Seminar Series: Cameras and Detectors
2024-03-26 13:00 - 14:00
μLectures is a new monthly seminar series designed to enhance researchers’ understanding of microscopy’s fundamental workings and principles. Each session, lasting 30 minutes, will focus on a specific concept or topic within microscopy. The practical information presented aims to deepen researchers’ comprehension of their microscopy experiments, giving them a better overview of the options available and enabling them to improve their experimental design and more effectively identify and resolve potential issues.
Annotation
The second session will focus on cameras and detectors. We will talk about the difference between cameras and detectors, and what parameters are important for which kind of experiments.

The seminar will take place on 26. 3. 2024 from 1 p.m. in the Turquoise Auditorium of the IEM CAS. It will also be possible to join online using this link (open in a new window).
Registration
Registration is not required, but we will be happy if you let us know (in person or at iva.svecova@iem.cas.cz) and if we have an idea of how many people are planning to come.
Lecturer
Štěpán Kortus, PhD.

Seminář Veronika Zimolová, M.Sc.
2024-03-27 15:00 - 16:00 | Posluchárna Milana Haška / Milan Hašek Auditorium
“Janus Kinase 2 (JAK2) mutations: implications for blood diseases”
The project aims to elucidate the impact of the germline Jak2 R1063H variant on myeloproliferative disorders and its potential implication in myeloid malignancy predisposition. Utilizing a mouse model harboring the Jak2 R1063H mutation, we observed higher mortality rate, elevated D-dimer levels indicative of thrombotic events, and increased platelets counts. Furthermore, metabolic profiling revealed alterations in platelet bioenergetics and gene expression patterns associated with fatty acid metabolism. Additionally, we showed aberrations in hematopoietic stem cell functionality, manifesting as a skewed myeloid progenitor cell expansion and bone marrow remodeling. These findings collectively suggest that Jak2 R1063H variant represents risk factor in the pathogenesis of myeloid cell transformation.

Seminář Jose Ordovas-Montanes, M.Sc., Ph.D.
2024-04-03 15:00 - 16:00 | Posluchárna Milana Haška / Milan Hašek Auditorium
“Single-cell to tissue-scale memory of infection and inflammation”
The role of epithelial and stromal cells in driving inflammation, together with immune cells, is being increasingly appreciated. However, we currently lack tissue-scale frameworks to define and test how inflammation collectively impacts epithelial, stromal, and immune cells. This poses a fundamental challenge for understanding the cellular mechanisms that drive or sustain health and disease within, and across, barriers such as the skin, airway, and intestine. Our lab is addressing this challenge by quantifying and controlling how inflammation changes the ecology of barrier tissues. Our central hypothesis is that inflammation is distributed and stored in multiple cell types, with the capacity to form tissue-level collective cellular networks. Starting from our human single-cell RNA-sequencing data collected from infectious and inflammatory diseases, we are developing quantitative approaches to define how static cellular ecosystems are arranged in barrier tissues. These studies are informing time-series modeling of murine inflammatory models focused on Type 1, 2 and 17 inflammation to understand how the same pressure applied to epithelial, stromal, and immune cell subsets shifts functional states and interactions in distinct barrier tissues. We anticipate to identify the gradual inflammatory transitions which maintain essential tissue functionality and critical “tipping points” in tissue state that compromise barrier functions.

IEM CAS Institutional Seminar Series 04/2024
2024-04-04 14:00 - 14:30
Maternal diet quality and health status of newborns
Lecturer: Mgr. Jitka Pavlíková, Ph.D. / Department of Toxicology a Molecular Epidemiology
Annotation: Persistent organic pollutants are compounds of mostly anthropogenic origin that persist in the environment for a long time, become part of food chains, and some of them accumulate in living organisms. These substances are being monitored for their adverse effects on organisms, and the use of some of them has already been banned or at least significantly restricted. In our study, we analyzed possible associations between the health status of newborns (birth weight, oxidative damage levels) and maternal diet. We obtained information about diet quantity and quality from the maternal diet records and determined the concentrations of 67 different persistent organic pollutants in the collected samples.
The seminar will take place on 4. 4. 2024 from 2 p.m. in the Turquoise Auditorium of the IEM CAS.
We look forward to your participation.

Lecture "Cancer metabolism"
2024-04-08 15:00 - 16:00 | Kinosal, Vídeňská 1083, Praha
RNDr. Katarína Smolková, Ph.D., Laboratory of Mitochondrial Physiology, IPHYS

Seminář Takashi Akera, Ph.D.
2024-04-10 15:00 - 16:00 | Posluchárna Milana Haška / Milan Hašek Auditorium
“The impact of genetic cheating on mammalian reproduction and speciation”
Selfish DNA violate Mendel’s Law of Segregation by biased segregation to the egg cell. This genetic cheating, meiotic drive, is a widespread phenomenon across eukaryotes with associated fitness costs such as fertility issues. I will discuss our recent work on the cell biological mechanism of meiotic drive and how it can impact mammalian reproduction and speciation.

Lecture "How Foxk1 and Foxk2 Regulate Aerobic Glycolysis – Implications for Systemic Energy Homeostasis and Type 2 Diabetes"
2024-04-15 15:00 - 16:00 | Kinosal, Vídeňská 1083, Praha
Prof. Sven Enerbäck, Department of Medical Biochemistry and Cell Biology, Institute of Biomedicine, University of Gothenburg, Sweden.

Seminář Kristýna Hrychová, M.Sc.
2024-04-17 15:00 - 16:00 | Posluchárna Milana Haška / Milan Hašek Auditorium
“Chromatin ADP-ribosylation in neurodegenerative disease”
ADP-ribosylation is a common protein post-translational modification that regulates numerous cellular processes, including transcription, cell proliferation and differentiation, immune and stress responses, aging, and cell death. One of its most extensively studied roles is in DNA damage response and subsequent DNA repair. Moreover, deregulation of ADP-ribose metabolism is linked to neurological and neurodevelopmental disorders. Interestingly, our recent work reveals that serine ADP-ribosylation is largely dispensable for DNA repair. We propose that deregulated serine ADP-ribosylation might instead lead to alterations in other histone modifications, changes in chromatin remodeling and gene expression, and consequently could contribute to the development of neurological diseases.

Lecture "Epoxylipids from biological actions to therapies for cardiovascular, kidney, and metabolic diseases"
2024-04-23 14:00 - 15:00 | Kinosal, Vídeňská 1083, Praha
Prof. John D. Imig, University of Arkansas for Medical Sciences, USA

Seminář Farahdokht Nourisanami, M.Sc.
2024-04-24 15:00 - 16:00 | Posluchárna Milana Haška / Milan Hašek Auditorium
“Molecular mechanisms of proteoglycan-mediated semaphorin signaling in axon guidance”
The precise assembly of a functional nervous system relies on axon guidance cues. Beyond engaging their cognate receptors and initiating signaling cascades that modulate cytoskeletal dynamics, guidance cues also bind components of the extracellular matrix, notably proteoglycans, yet the role and mechanisms of these interactions remain poorly understood. We found that Drosophila secreted semaphorins bind specifically to glycosaminoglycan (GAG) chains of proteoglycans, showing a preference based on the degree of sulfation. Structural analysis of Sema2b unveiled multiple GAG binding sites positioned outside canonical plexin-binding site, with the highest affinity binding site located at the C-terminal tail, characterized by a lysine-rich helical arrangement that appears to be conserved across secreted semaphorins. In vivo studies revealed a crucial role of the Sema2b C-terminal tail in specifying the trajectory of olfactory receptor neurons. We propose that secreted semaphorins tether to the cell surface through interactions with GAG chains of proteoglycans, facilitating their presentation to cognate receptors on passing axons.

Lecture "Redox mechanisms of insulin secretion"
2024-04-29 15:00 - 16:00 | Kinosal, Vídeňská 1083, Praha
RNDr. Petr Ježek, DrSc., Laboratory of Mitochondrial Physiology, IPHYS

IEM CAS Distinguished Guest Seminar Series - Cyril Hanus
2024-04-30 10:00 - 12:00
The dendrite’s sweet spot: Atypical glycosylation of the neuronal surface and impact on synaptic receptor trafficking, structure and function
Lecturer: Cyril Hanus, PhD. / The Institute of Psychiatry and Neuroscience of Paris
The seminar will take place on 30. 04. 2024 from 10:00 p.m. in the Turquoise Auditorium of the IEM CAS.
Annotation:
Like 75% of membrane proteins, neurotransmitter receptors and synaptic adhesion molecules are literally covered by covalently linked sugars, N-glycans. Despite a general awareness that N-glycans may have a strong impact on protein structure and function and are altered in a number of brain pathologies, we know surprisingly little about N-glycosylation in the brain. Our work indicates that a substantial fraction of neuronal surface proteins, notably glutamate AMPA receptors, are locally synthesized in dendrites and use unconventional Golgi-independent pathways to reach the neuronal surface. This local processing modifies protein N-glycosylation states and hence regulates receptor chemical composition and function, unravelling a previously unrecognized mechanism controlling the sensing properties and plasticity of the neuronal membrane.
Keywords: cell-biology, neurotransmitter receptors, synaptic plasticity, cell type-specific gene knockout, electrophysiology, mass spectrometry, molecular dynamics, structural biology
We look forward to your participation.

IEM CAS μLectures Seminar Series: Confocal Microscopy
2024-04-30 13:00 - 14:00
μLectures is a monthly seminar series designed to enhance researchers’ understanding of microscopy’s fundamental workings and principles. Each session, lasting 30 minutes, focuses on a specific concept or topic within microscopy. The practical information presented aims to deepen researchers’ comprehension of their microscopy experiments, giving them a better overview of the options available and enabling them to improve their experimental design and more effectively identify and resolve potential issues.
Annotation
This session will focus on confocal microscopy. We will talk about the basic principles of confocal microscopy, its practical implications and the difference between different confocal microscopy techniques (scanning vs spinning disk).
The seminar will take place on 30. 4. 2024 from 1 p.m. in the Turquoise Auditorium of the IEM CAS. It will also be possible to join online using this link (open in a new window).
Registration
Registration is not required, but we will be happy if you let us know (in person or at iva.svecova@iem.cas.cz) and if we have an idea of how many people are planning to come.
Lecturer
Barbora Hyklová, PhD.

IEM CAS Institutional Seminar Series 05/2024
2024-05-02 14:00 - 15:00
Monitoring of telomeres through colorectal adenomas to advanced stages of colorectal cancer
Lecturer: Mgr. Kateřina Balounová / Department of the Molecular Biology of Cancer
Annotation: Telomeres, repetitive sequences located at the ends of chromosomes, naturally shorten with ageing. Disruption in telomere biology is closely associated with the onset and progression of cancers, including colorectal carcinoma (CRC). Given that CRC is one of the most common cancer-related deaths, we focus on studying telomeres and their potential use to characterise CRC subtypes, as well as serving as a suitable prognostic biomarker for this disease.
The seminar will take place on 2. 5. 2024 from 2 p.m. in the Turquoise Auditorium of the IEM CAS.
We look forward to your participation.

Lecture "Preclinical Drug Development for Epilepsy: Animal Models and New Horizons"
2024-05-09 15:00 - 16:00
Cameron S.Metcalf, Dept.Pharmacology, University of Utah, Salt Lake City, USA
venue: Conference room of Laboratory of Biomathematics in Krč areal (Vídeńská 1083)

Lectures of Laboratory of Developmental Cardiology
2024-05-13 15:00 - 16:00 | Kinosal, Vídeňská 1083, Praha
Vevo 3100: The Short Story of a High-tech Upgrade
Jaroslav Hrdlička, Laboratory of Developmental Cardiology, IPHYS

Cardioprotection: the role of HIF-1α and mitochondria
Petra Alánová, Laboratory of Developmental Cardiology, IPHYS

3rd 10x Genomics Experience Meeting 2024
2024-05-14 08:30 - 16:30 | Posluchárna Milana Haška / Milan Hašek Auditorium
