Představení kandidáta na předsedu AV ČR pro funkční období 2025–2029 - prof. RNDr. David Honys, Ph.D.
2024-09-10 14:00 - 15:30 (Europe/Prague)
Dne 24. 3. 2025 skončí druhé čtyřleté období ve funkci předsedkyně AV ČR prof. RNDr. Evě Zažímalové, CSc. Volba kandidáta na předsedu AV ČR pro následné funkční období proběhne na zasedání sněmu AV ČR dne 10. 12. 2024.
Jedním z kandidátů na předsedu AV ČR je prof. RNDr. David Honys, Ph.D., který představí zaměstnancům krčského kampusu svou vizi o řízení AV ČR na jednání v úterý 10. 9. 2024 od 14:00 hod. v sále Milana Haška na ÚMG.

IEM CAS Distinguished Guest Seminar Series – Prof. Kai Liu
2024-07-18 15:00 - 16:30
Lecturer: Prof. Kai Liu / Division of Life Science, Hong Kong University of Science and Technology
The seminar will take place on 18. 07. 2024 from 15:00 in the Turquoise Auditorium of the IEM CAS.
Introduction:
Prof. Kai Liu received his Bachelor degree from School of Life Sciences at Peking University in 1998, and received his Ph.D. from Rutgers University at New Brunswick in 2006. Then he did his postdoc research at Children’s Hospital Boston/Harvard Medical School. He joined Division of Life Science at HKUST as Assistant Professor in 2011, and is currently Cheng Associate Professor of Science. His research interest focuses on the intrinsic mechanisms regulating axonal regeneration and functional repair after central nervous system injuries.

IEM CAS Distinguished Guest Seminar Series – Cian O'Connor
2024-07-18 14:00 - 15:00
Lecturer: Cian O’Connor, PhD. / Tissue engineering research group, University of Medicine and Health Sciences Dublin
The seminar will take place on 18. 07. 2024 from 14:00 in the Turquoise Auditorium of the IEM CAS.
Introduction:
Cian O’Connor is a postdoctoral researcher in the Tissue engineering research group led by Prof. Fergal O’Brien, RCSI & physiology graduate from Trinity College Dublin with an interest in cellular neuroscience and biomaterials. His interest lies in studying glial cells and technological advancements associated with therapeutic treatments particularly via stem cell culture, development of scaffold implants, the use of non-viral gene delivery and electroconductive materials.
We look forward to your participation.

Seminář Prof. Dirk Schübeler
2024-06-26 16:00 - 17:00 | Posluchárna Milana Haška / Milan Hašek Auditorium
“Finding your place: Transcription factors as sensors and modifiers of chromatin”
Transcription factors only bind a minority of their motifs in large mammalian genomes. One likely explanation is that many motifs are not accessible for binding due to the action of chromatin and DNA methylation. We are using mammalian stem cell models and their differentiation combined with genomics to understand this important interplay between gene regulation, chromatin structure and DNA methylation. We study the dynamics of the epigenome and test regulatory models in cellular models by genetic perturbation and genome editing approaches.
I will discuss our recent efforts in understanding how the sensitivity to DNA methylation and nucleosomes can limit transcription factor binding in the context of the cell and how TFs rely on specific chromatin remodelers for access to their binding sites.

IEM CAS μLectures: Checklists for Publishing Images and Introduction to Image Archives
2024-06-25 13:00 - 14:00
μLectures is a monthly seminar series designed to enhance researchers‘ understanding of microscopy’s fundamental workings and principles. Each session, lasting 30 minutes, focuses on a specific concept or topic within microscopy. The practical information presented aims to deepen researchers‘ comprehension of their microscopy experiments, giving them a better overview of the options available and enabling them to improve their experimental design and more effectively identify and resolve potential issues.
Annotation
Thanks to speedy technological developments, microscopy images document a significant part of scientific discoveries in this data-intensive era. It is becoming increasingly important to ensure that images are presented in a transparent and reproducible manner. However, there are only a few guidelines available for publishing terabytes to petabytes of image data, while research funding agencies require data to be published in open repositories. The objective of this seminar is to provide an overview of the key aspects of data publication, with a particular focus on image data. In addition, the seminar will offer a brief introduction to repositories and other relevant resources.
The seminar will take place on 25. 6. 2024 from 1 p.m. in the Turquoise Auditorium of the IEM CAS. It will also be possible to join online using this link.
Registration
Registration is not required, but we will be happy if you let us know (in person or at iva.svecova@iem.cas.cz) and if we have an idea of how many people are planning to come.

Představení kandidáta na předsedu AV ČR pro funkční období 2025–2029 - Ing. Jiří Plešek, CSc.
2024-06-20 10:00 - 11:30 | Posluchárna Milana Haška / Milan Hašek Auditorium
Dne 24. 3. 2025 skončí druhé čtyřleté období ve funkci předsedkyně AV ČR
prof. RNDr. Evě Zažímalové, CSc. Volba kandidáta na předsedu AV ČR pro následné funkční období proběhne na zasedání sněmu AV ČR dne 10. 12. 2024.

Jedním z kandidátů na předsedu AV ČR je Ing. Jiří Plešek, CSc.

Seminář Josef Novák, Ph.D.
2024-06-19 15:00 - 16:00 | Posluchárna Milana Haška / Milan Hašek Auditorium
“Switch of ADP/ATP translocase isoforms in cellular senescence and malignancy”
Four isoforms of human ATD/ADP translocators (ANTs) have poorly understood functional differences. Due to their high sequence similarity, antibody-based detection of these proteins is challenging. We, therefore, developed a sensitive proteomic method for detecting ATD/ADP translocator isoforms and their relative ratio. Using this technique, we observed a switch of ANT isoforms during senescence establishment associated with changes in cellular energy metabolism. The switch of ANT protein isoforms was not correlated with the change of ANT transcripts. Similarly, we found a non-correlation of ANT transcripts with corresponding proteins in patients‘ glioblastoma and lung cancer samples. We concluded that protein levels of ANT isoforms are regulated on multiple levels and are tightly linked to mitochondria biogenesis.

Lecture "Obesity cannot be cured, but increasingly better treated"
2024-06-17 16:30 - 17:30 | Kinosal, Vídeňská 1083, Praha
Prof. Matthias Blüher, Director of the Helmholtz Institute for Metabolism, Obesity and Vasculature research at the Helmholtz Center Munich and the University of Leipzig, Germany

Seminář Jiří Březina, M.Sc.
2024-06-12 15:00 - 16:00 | Posluchárna Milana Haška / Milan Hašek Auditorium
“How antigens get distributed in the thymus”
The presentation of self-antigens in the thymus by medullary thymic epithelial cells (mTECs) and dendritic cells (DCs) is crucial for the establishment of central tolerance. While mTECs produce and present self-antigens in an autonomous manner, DCs acquire them from mTECs by cooperative antigen transfer (CAT). Although CAT represents a key process of central tolerance, molecular determinants which define CAT’s effectivity as well as the the capacity of DCs to subsequently present highjacked antigens are largely unknown. To reveal the nature of these determinants, we compared the transcriptomes of CAT-experienced and inexperienced DCs via scRNAseq. In this seminar, we will focus on one such a determinant of CAT, gene product encoding the tight junction protein, CLAUDIN 1. A series of experiments demonstrated that CLAUDIN 1 is a regulatory molecule which coordinates CAT and T cell selection processes. The comprehension of these processes will aid in the resolution of the current controversy concerning the role of DCs in central tolerance.

IEM CAS μLectures Seminar Series: Digital Image Basics
2024-06-12 13:00 - 14:00
μLectures is a monthly seminar series designed to enhance researchers’ understanding of microscopy’s fundamental workings and principles. Each session, lasting 30 minutes, focuses on a specific concept or topic within microscopy. The practical information presented aims to deepen researchers’ comprehension of their microscopy experiments, giving them a better overview of the options available and enabling them to improve their experimental design and more effectively identify and resolve potential issues.
Annotation
The seminar aims to provide participants with the basic knowledge and skills to work with graphics and digital images. The participants will be introduced to key concepts and techniques. For example, they will learn how information is stored in a digital image or what bit depth is. Understanding these principles is essential for the proper processing of microscopic data.
The seminar will take place on 12.6.2024 from 1 p.m. in the Turquoise Auditorium of the IEM CAS. It will also be possible to join online using this link.
Registration
Registration is not required, but we will be happy if you let us know (in person or at iva.svecova@iem.cas.cz) and if we have an idea of how many people are planning to come.
Lecturer
Jan Setnička

Practical course SUPER-RESOLUTION IN LIGHT MICROSCOPY
2024-06-12 - 2024-06-14 | IMG

IEM CAS Institutional Seminar Series 06/2024
2024-06-06 14:00 - 15:00
Open Access Publishing & Green Open Access
Lecturer: Arzuv Caryjeva, Ph.D. / Data Manager / Director’s Secretariat
Annotation: The Open Science requirements of project funders and available options and tools to satisfy these requirements will be introduced. Mainly, Open access publishing and the green route to ensure open access to Scientific articles will be discussed. Moreover, there will be an introduction to the Institutional data repository and how researchers can use it. This seminar is particularly important for lab leaders and grant holders.
The seminar will take place on 6. 6. 2024 from 2 p.m. in the Turquoise Auditorium of the IEM CAS.
We look forward to your participation.

Seminář Subhadip Chakraborty, M.Sc.
2024-06-05 15:00 - 16:00 | Posluchárna Milana Haška / Milan Hašek Auditorium
“Understanding the mechanochemical transitions that underlie early embryo development”
During embryogenesis a single fertilized oocyte develops into an adult organism via tightly controlled patterns of cell division, cell polarization and cell movements. These mechanical processes depend on the actomyosin cortex, a thin 2D layer that resides underneath the plasma membrane. Importantly, for normal development the actomyosin cortex in rapidly dividing embryonic cells needs to transition between contractile and non-contractile phases. Surprisingly little is known about how these mechanical transitions are regulated in a developing embryo. To understand mechanical transitions during embryogenesis, I use C. elegans early embryos as a model, and perform quantitative live imaging of the actomyosin cortex as it transitions between contractile and non-contractile phases. In the IMG seminar I will present my recent results in which we demonstrate how two major signaling cascades, Cdc42 and RhoA, affect distinct mechanical phases and the transitions between them.

Lecture "How are the epilepsy molecular clocks ticking?"
2024-06-03 15:00 - 16:00 | Kinosal, Vídeňská 1083, Praha
Cristina Reschke, RCSI University of Medicine and Health Sciences, School of Pharmacy and Biomolecular Sciences, Dublin, Ireland

Seminář Prof. Dominique Bonnet
2024-05-29 15:00 - 16:00 | Posluchárna Milana Haška / Milan Hašek Auditorium
“Dissecting the cross-talk between normal, leukaemic stem cells and the bone marrow microenvironment”
Haematopoietic stem cells (HSC) sustain the life-long maintenance of mature blood cells. Over the last decade progress has been made in our understanding of the contribution of the different stroma cells in the regulation of normal HSCs. Our current knowledge is predominantly derived from mouse studies. In humans, the cellular and molecular composition of the bone marrow (BM) niche, its functional organisation remains largely unknown. In recent years, deregulation of the BM microenvironment has emerged to be an important factor in the development of myeloid malignancies. Indeed, like normal HSC, leukaemic stem cells (LSCs) remain dependent on signals from the BM niche for survival and proliferation, and niche factors serves as a sanctuary for their chemoresistance. Thus, targeting BM niche factors represents a promising avenue for therapeutic interventions. Determining the niche associated alterations in the composition/ organisation along with dissecting the interactome of LSCs and their niche is thus fundamental, which will ultimately enable us to understand the mechanism of leukaemia initiation, evolution and resistance.
I will summarise some of the recent published data from the lab, showing the alterations of endothelial cells by AML as well as described the development of our new humanised scaffold system that is allowing us to better study the cross-talk between normal and malignant HSPCs and their bone marrow niche in a human/human context. I will show the utility of this model to study MDS and their dependence to the human niche components. Lastly, I will also discuss new un/published data on the effect of pre-leukaemic mutation on haematopoieisis.

BioCEV-Krč Methodological Seminar
2024-05-27 15:00 - 16:00 | IMG
BioCEV-Krč Methodological Seminar
Topic: Campus Animal Facility
27. 5. 2024, start: 15:00
venue: Milan Hašek lecture hall, IMG CAS
The Animal facility methodological seminar will include a series of presentations by representatives of individual institutions located on the campus. Each institution will briefly present its local or detached animal facility and the services it provides

Seminář Vadym Sulimenko, Ph.D.
2024-05-22 15:00 - 16:00 | Posluchárna Milana Haška / Milan Hašek Auditorium
“Differential roles of GIT1 and GIT2 adaptor proteins in regulating microtubule nucleation in glioblastoma cells”
Microtubules play a critical role in cell division and migration, impacting the viability and invasiveness of malignant tumors. Although the reorganization of highly dynamic microtubules is central to these processes, the precise mechanism underlying microtubule involvement in glioblastoma cell motility remains poorly understood. G protein-coupled receptor kinase-interacting proteins (GITs) are GTPase-activating proteins (GAPs) for ADP-ribosylation factor (Arf) small GTPases. GITs are structurally conserved multidomain scaffold/adaptor proteins that link Arf GTPases to other intracellular signaling events. Here we show that the signalling proteins GIT1 and GIT2 form complexes with γ-Tubulin ring complexes (γTuRCs), associate with centrosomes and differentially regulate microtubule nucleation and cell migration in glioblastoma cells.

IEM CAS Entry Level Super-Resolution Microscopy
2024-05-22 - 2024-05-24
Basic Information
The course covers theoretical and practical aspects of an “entry-level” super-resolution imaging methods based on the principle of Optical-Photon-Reassignment available in our facility at confocal microscopes Zeiss LSM880
with AiryScan and Olympus SpinSR10 with OSR.
What is meant by “Entry-Level Super-Resolution Microscopy”? It is a group of methods that improve resolution by a factor of ~2 over diffraction-limited imaging and are very easy to use. While these methods cannot offer the resolution improvement of factor 5x-10x like STED or STROM/PALM, they are fast, easy to operate and require no special sample preparation or extra skills. They are also very suitable for live cell imaging.

Duration: 3 days
Language: English
Location: IEM library, microscope rooms

Registration form and more information can be found on our website.

Lecture "Single-particle tracking of neurotransmitter receptors as a window on synaptic organization and function"
2024-05-20 15:00 - 16:00 | Kinosal, Vídeňská 1083, Praha
RNDr. Martin Zápotocký, Ph.D., Laboratory of Computational Neuroscience, IPHYS

Seminář Milan Reiniš, Ph.D.
2024-05-15 15:00 - 16:00 | Posluchárna Milana Haška / Milan Hašek Auditorium
“Targeting the STAT3 signaling pathway in cancer and senescence”
STAT3 signaling pathway plays important roles in many cellular processes, such as cell differentiation, carcinogenesis, cellular senescence or regulation of the immune responses. The STAT3 molecule thus represents an attractive therapeutic target. However, its druggability is still questionable. We have tested a number of small molecules designed to inhibit STAT3 phosphorylation for their inhibitory capabilities and impacts on the tumor cell growth. Importantly, we have documented that selected compounds with limited cytotoxicity were able to inhibit secretion of immunosuppressive cytokines by senescent cells. These results suggest that the STAT3 inhibitors could serve as senomodulatory agents.

3rd 10x Genomics Experience Meeting 2024
2024-05-14 08:30 - 16:30 | Posluchárna Milana Haška / Milan Hašek Auditorium

Lectures of Laboratory of Developmental Cardiology
2024-05-13 15:00 - 16:00 | Kinosal, Vídeňská 1083, Praha
Vevo 3100: The Short Story of a High-tech Upgrade
Jaroslav Hrdlička, Laboratory of Developmental Cardiology, IPHYS

Cardioprotection: the role of HIF-1α and mitochondria
Petra Alánová, Laboratory of Developmental Cardiology, IPHYS

Lecture "Preclinical Drug Development for Epilepsy: Animal Models and New Horizons"
2024-05-09 15:00 - 16:00
Cameron S.Metcalf, Dept.Pharmacology, University of Utah, Salt Lake City, USA
venue: Conference room of Laboratory of Biomathematics in Krč areal (Vídeńská 1083)

IEM CAS Institutional Seminar Series 05/2024
2024-05-02 14:00 - 15:00
Monitoring of telomeres through colorectal adenomas to advanced stages of colorectal cancer
Lecturer: Mgr. Kateřina Balounová / Department of the Molecular Biology of Cancer
Annotation: Telomeres, repetitive sequences located at the ends of chromosomes, naturally shorten with ageing. Disruption in telomere biology is closely associated with the onset and progression of cancers, including colorectal carcinoma (CRC). Given that CRC is one of the most common cancer-related deaths, we focus on studying telomeres and their potential use to characterise CRC subtypes, as well as serving as a suitable prognostic biomarker for this disease.
The seminar will take place on 2. 5. 2024 from 2 p.m. in the Turquoise Auditorium of the IEM CAS.
We look forward to your participation.

IEM CAS μLectures Seminar Series: Confocal Microscopy
2024-04-30 13:00 - 14:00
μLectures is a monthly seminar series designed to enhance researchers’ understanding of microscopy’s fundamental workings and principles. Each session, lasting 30 minutes, focuses on a specific concept or topic within microscopy. The practical information presented aims to deepen researchers’ comprehension of their microscopy experiments, giving them a better overview of the options available and enabling them to improve their experimental design and more effectively identify and resolve potential issues.
Annotation
This session will focus on confocal microscopy. We will talk about the basic principles of confocal microscopy, its practical implications and the difference between different confocal microscopy techniques (scanning vs spinning disk).
The seminar will take place on 30. 4. 2024 from 1 p.m. in the Turquoise Auditorium of the IEM CAS. It will also be possible to join online using this link (open in a new window).
Registration
Registration is not required, but we will be happy if you let us know (in person or at iva.svecova@iem.cas.cz) and if we have an idea of how many people are planning to come.
Lecturer
Barbora Hyklová, PhD.

IEM CAS Distinguished Guest Seminar Series - Cyril Hanus
2024-04-30 10:00 - 12:00
The dendrite’s sweet spot: Atypical glycosylation of the neuronal surface and impact on synaptic receptor trafficking, structure and function
Lecturer: Cyril Hanus, PhD. / The Institute of Psychiatry and Neuroscience of Paris
The seminar will take place on 30. 04. 2024 from 10:00 p.m. in the Turquoise Auditorium of the IEM CAS.
Annotation:
Like 75% of membrane proteins, neurotransmitter receptors and synaptic adhesion molecules are literally covered by covalently linked sugars, N-glycans. Despite a general awareness that N-glycans may have a strong impact on protein structure and function and are altered in a number of brain pathologies, we know surprisingly little about N-glycosylation in the brain. Our work indicates that a substantial fraction of neuronal surface proteins, notably glutamate AMPA receptors, are locally synthesized in dendrites and use unconventional Golgi-independent pathways to reach the neuronal surface. This local processing modifies protein N-glycosylation states and hence regulates receptor chemical composition and function, unravelling a previously unrecognized mechanism controlling the sensing properties and plasticity of the neuronal membrane.
Keywords: cell-biology, neurotransmitter receptors, synaptic plasticity, cell type-specific gene knockout, electrophysiology, mass spectrometry, molecular dynamics, structural biology
We look forward to your participation.

Lecture "Redox mechanisms of insulin secretion"
2024-04-29 15:00 - 16:00 | Kinosal, Vídeňská 1083, Praha
RNDr. Petr Ježek, DrSc., Laboratory of Mitochondrial Physiology, IPHYS

Seminář Farahdokht Nourisanami, M.Sc.
2024-04-24 15:00 - 16:00 | Posluchárna Milana Haška / Milan Hašek Auditorium
“Molecular mechanisms of proteoglycan-mediated semaphorin signaling in axon guidance”
The precise assembly of a functional nervous system relies on axon guidance cues. Beyond engaging their cognate receptors and initiating signaling cascades that modulate cytoskeletal dynamics, guidance cues also bind components of the extracellular matrix, notably proteoglycans, yet the role and mechanisms of these interactions remain poorly understood. We found that Drosophila secreted semaphorins bind specifically to glycosaminoglycan (GAG) chains of proteoglycans, showing a preference based on the degree of sulfation. Structural analysis of Sema2b unveiled multiple GAG binding sites positioned outside canonical plexin-binding site, with the highest affinity binding site located at the C-terminal tail, characterized by a lysine-rich helical arrangement that appears to be conserved across secreted semaphorins. In vivo studies revealed a crucial role of the Sema2b C-terminal tail in specifying the trajectory of olfactory receptor neurons. We propose that secreted semaphorins tether to the cell surface through interactions with GAG chains of proteoglycans, facilitating their presentation to cognate receptors on passing axons.

Lecture "Epoxylipids from biological actions to therapies for cardiovascular, kidney, and metabolic diseases"
2024-04-23 14:00 - 15:00 | Kinosal, Vídeňská 1083, Praha
Prof. John D. Imig, University of Arkansas for Medical Sciences, USA

Seminář Kristýna Hrychová, M.Sc.
2024-04-17 15:00 - 16:00 | Posluchárna Milana Haška / Milan Hašek Auditorium
“Chromatin ADP-ribosylation in neurodegenerative disease”
ADP-ribosylation is a common protein post-translational modification that regulates numerous cellular processes, including transcription, cell proliferation and differentiation, immune and stress responses, aging, and cell death. One of its most extensively studied roles is in DNA damage response and subsequent DNA repair. Moreover, deregulation of ADP-ribose metabolism is linked to neurological and neurodevelopmental disorders. Interestingly, our recent work reveals that serine ADP-ribosylation is largely dispensable for DNA repair. We propose that deregulated serine ADP-ribosylation might instead lead to alterations in other histone modifications, changes in chromatin remodeling and gene expression, and consequently could contribute to the development of neurological diseases.
