Seminář - RNDr. Ondřej Mihola, Ph.D.
2023-11-22 15:00 - 16:00 (Europe/Prague) | Posluchárna Milana Haška / Milan Hašek Auditorium
“A chance for fertility despite bad DNA breaks (AKA The effect of crossing-over rate on fertility)”
Programmed meiotic DNA breaks are essential for mammalian fertility but dangerous for genome integrity. DNA-binding H3-lysine-trimethyltransferase PRDM9 determines the positions of the meiotic DNA breaks, which can be repaired as crossovers or noncrossovers. The crossover/noncrossover decision is regulated by many factors that include RING-domain E3 ligase RNF212. The dosage of the Rnf212 gene in mouse affects the crossover rate per meiotic cell and variants in the human RNF212 gene are associated with crossover rates. Deficiency for RNF212 leads to complete arrest of meiosis at the metaphase stage. The deficiency for human and rodent PRDM9 causes partial to complete pachytene arrest, depending on unknown genetic factors. We will present results demonstrating that the dosage of Rnf212 (and thus crossover rate) affects meiotic progression and germ cell counts of semisterile Prdm9-deficient mice. Our findings offer an insight into the mechanisms and treatment possibilities of some cases of infertility.

Lecture
2023-11-20 15:00 - 16:00
Laboratory of Developmental Epileptology, IPHYS

IEM CAS Distinguished Guest Seminar Series - Prof. Jens Pahnke
2023-11-20 14:30 - 16:00
ABC transporter blood-brain barrier function in neurodegenerative and neuroinflammatory diseases – from basic research to treatment
Lecturer: Prof. Jens Pahnke, M.D., Ph.D., E.F.N./ University of Oslo, Faculty of Medicine / Oslo University Hospital, Department of Pathology, Norway
The seminar will take place on 20. 11. 2023 from 2:30 p.m. in the Turquoise Auditorium of the IEM CAS.
Annotation:
The current understanding of neurodegenerative diseases focusses on the deposition of toxic peptide species as larger aggregates in the brain of effected individuals. However, patients with sporadic neurodegenerative diseases do not overexpress these peptides. Moreover, they are hampered in removing the physiologically produced peptides, e.g. Aβ and α-synuclein. The blood-brain barrier plays a prominent role in the active and effective removal of metabolites and in the efflux of toxic peptides from the brain into the blood stream.
Here, ABC transporters were discovered by us and others to have a fundamental role in the homeostasis of the brain’s environment. In 2011, we described a new ABC transporter (ABCC1) that increases Aβ in the brain of knock-out animals by 12-14fold. Since then, we could show that the activation of ABC transporters can be used as a diagnostic tool and a new treatment option in elderly with Alzheimer’s disease and other neurodegenerative diseases, such as Huntington’s disease.
1 Pahnke, J. et al. Free Neuropathol 2, 33, (2021)
2 Pahnke, J. et al. Neurobiol Dis 72 Pt A, 54-60, (2014)
3 Pahnke, J. et al. Curr Alzheimer Res 5, 396-405, (2008)
We look forward to your participation.

Seminář - Prof. Jiří Forejt, M.D., Ph.D., DSc.
2023-11-15 15:00 - 16:00 | Posluchárna Milana Haška / Milan Hašek Auditorium
“Genetic architecture of Prdm9-driven reproductive isolation between mouse subspecies”
Hybrid incompatibility, the inviability or infertility of species hybrids, has played a central role in speciation research since Darwin’s Origin of Species. However, the search for genes controlling hybrid sterility, mainly using Drosophila species hybrids, has been largely unsuccessful, revealing a polygenic control with complex epistatic interactions. Fifty years ago, we identified the first hybrid sterility locus, Hst1, in the house mouse genome, and 14 years ago, we positionally cloned Prdm9 as the gene responsible for Hst1-controlled hybrid sterility.
In this talk, I will summarize our current understanding of the molecular genetic mechanisms that control this unique model of mammalian hybrid sterility.

Den otevřených dveří ÚMG / IMG Open House Day
2023-11-14 09:00 - 15:00

Lecture "Gliflozins in the treatment of non-diabetic cardiovascular diseases"
2023-11-13 15:00 - 16:00 | Kinosal, Vídeňská 1083, Praha
RNDr. Ivana Vaněčková, DSc., Laboratory of Experimental Hypertension, IPHYS

IEM CAS Open House Day / Week of the Czech Academy of Sciences Festival
2023-11-10
Week of the Czech Academy of Sciences is a science festival that follows the previous Week of Science and Technology of the CAS. The festival offers scientific lectures, exhibitions, events at institutes, documentary films, workshops and many other activities across the Czech Republic and all scientific disciplines. As part of the festival, the IEM CAS traditionally organises an Open House Day, which includes individual and group excursions around the research departments, as well as a cycle of lectures which presents the latest discoveries and achievements of the IEM CAS to the public. The morning programme is focused primarily on high school students.
Registration

Den otevřených dveří - FGÚ
2023-11-09 | Kinosal, Vídeňská 1083, Praha
Začátek v 9,00 hod a ve 13,00 hod.

Seminář – Assoc. Prof. Pavel Plevka
2023-11-08 15:00 - 16:00 | Posluchárna Milana Haška / Milan Hašek Auditorium

Lecture "Clocks in our body – how do they synchronize to shape our physiology?"
2023-11-06 15:00 - 16:00 | Kinosal, Vídeňská 1083, Praha
Prof. PharmDr. Alena Sumová, DSc., Laboratory of Biological Rhythms, IPHYS

IEM CAS Institutional Seminar Series 11/2023
2023-11-02 14:00 - 14:45
Single-cell gene expression analysis of developing craniofacial region
Lecturer: Erika Hudáčová, M.Sc. / Department of Developmental Biology
The seminar will take place on 2. 11. 2023 from 2 p.m. in the Turquoise Auditorium of the IEM CAS.
Annotation: In vertebrates, the skull is composed of various skeletal elements originating from cranial neural crest cells (CNCCs). CNCCs are responsible for the formation of the cranium vault, frontonasal prominence, upper and lower jaw, hyoid bone, external ear and middle ear. The morphogenesis of the craniofacial region encompasses a number of processes, from epithelial-mesenchymal induction and mesenchymal condensation to endochondral or intramembranous ossification. However, the molecular and genetic pathways regulating cranial cartilage shaping and growth during embryogenesis are poorly understood.
In our research, we present Wnt1-Cre2, Meis2 conditional knock-out (cKO) mice with several craniofacial bone abnormalities resembling severe human pathologies due to improper molecular mechanisms guiding mesenchymal condensation. Using single-cell RNA sequencing, we further focus on describing the Meis2-dependent gene regulatory network involved in mesenchymal condensation. Comparison of wild-type and Meis2 cKO embryonic stages E12.5 and E13.5 reveals multiple mesenchymal cell types responsible for craniofacial formation. We have identified markers for cell type specification and positional cues in developing craniofacial regions. Furthermore, differences identified in specific cell types governing mesenchymal condensation and endochondral ossification correlate with the phenotype present in Meis2 cKO mice. Our transcriptomics data in Meis2 cKO show elevated expression of the genes involved in ossification, cell-cell adhesion and cell-extracellular matrix contact. Altogether, the identification of hitherto unknown factors controlling the growth and shape of cranial bones may potentially contribute to the mitigation or prevention of human craniofacial abnormalities.
We look forward to your participation.

Lecture "Understanding the molecular mechanisms of thermoTRP ion channel activation"
2023-10-23 15:00 - 16:00 | Kinosal, Vídeňská 1083, Praha
Mgr. Lucie Zímová, Ph.D., Laboratory of Cellular Neurophysiology

Lecture "Parvalbumin interneurons: A hothouse flower in the brain?"
2023-10-16 15:00 - 16:00 | Kinosal, Vídeňská 1083, Praha
RNDr. Jan Svoboda Ph.D. – Laboratory of Neurophysiology of Memory

Bureš lecture series "Skeletal muscle function in the context of the neuromuscular, musculoskeletal and metabolic system"
2023-10-09 15:00 - 16:30 | Kinosal, Vídeňská 1083, Praha
Prof. Stefano Schiaffino, Venetian Institute of Molecular Medicine, Padova, Italy

Pravidelný středeční seminář / Regular Wednesday Seminar
2023-10-04 15:00 - 16:00 | Posluchárna Milana Haška / Milan Hašek Auditorium

Miloš Pěkný - Astrocytes as a treatment target in neurological diseases Marcela Pěkná - Novel therapeutic strategy to promote recovery after stroke
2023-10-02 12:30 - 15:00 | Posluchárna Milana Haška / Milan Hašek Auditorium

NIVB Networking Meeting
2023-08-21
