Granulocyte Colony-Stimulating
Factor and Drugs Elevating Extracellular Adenosine Act
Additively to Enhance the Hemopoietic Spleen Colony Formation in
Irradiated Mice
M. HOFER, M. POSPÍŠILl, J. NETÍKOVÁ,
V. ZNOJIL1, J. VÁCHA1
Institute of Biophysics, Academy of Sciences of the Czech
Republic and 1Institute of Pathological Physiology, Medical
Faculty, Masaryk University, Brno, Czech Republic
Received May 7, 1998
Accepted October 1, 1998
Summary
The effects of combined administration of two drugs elevating
extracellular adenosine, namely dipyridamole (DP) and adenosine
monophosphate (AMP), and granulocyte colony-stimulating factor
(G-CSF) on hemopoietic stem cells in vivo were investigated. The
experiments were performed on mice using the endogenous spleen
colony formation in gamma-irradiated animals as an endpoint. The
results have shown that DP and AMP act additively with G-CSF to
enhance spleen colony formation and thus the erythroid
repopulation of the spleen. These findings indicate that the
signaling pathways of G-CSF and drugs elevating extracellular
adenosine can interact at the level of primitive hemopoietic
stem cells. The enhancement of hemopoiesis-stimulating effects
of G-CSF by DP and AMP, which are low-priced and clinically
available drugs, could improve the cost-effectiveness of the
therapy with G-CSF.
Key words
Hemopoietic stem cells - Granulocyte colony-stimulating factor -
Dipyridamole - Adenosine monophosphate
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