Physiol. Res. 48: 113-117, 1999

Lipolysis Induced by Alloxan in Rat Adipocytes is Not Inhibited by Insulin

K. KANDULSKA, T. SZKUDELSKI, L. NOGOWSKI

Department of Animal Physiology and Biochemistry, University of Agriculture, Poznań, Poland

Received June 12, 1998
Accepted January 7, 1999


Summary
Isolated rat adipocytes were incubated with adrenaline, adrenaline plus insulin, alloxan or alloxan plus insulin. Glycerol release was taken as a measure of lipolysis. It was observed that alloxan in the concentration of 3, 10 and 20 mmol/l intensifies lipolysis in adipocytes in the absence of adrenaline. Insulin (10-6 mol/l) treatment of cells did not inhibit lipolysis caused by this compound, but significantly restricted lipolysis induced by adrenaline (10-6 mol/l). It was also shown that alloxan in the concentration of 3 and 10 mmol/l intensified lipolysis stimulated by adrenaline (10-6 mol/l). Addition of 20 mmol/l of alloxan strongly inhibited glycerol release in the presence of adrenaline. The results presented here clearly indicate that the action of alloxan concerns cells of the white adipose tissue.


Key words
Adipocytes · Alloxan · Lipolysis · Adrenaline · Insulin · Rat


© 1999 by the Institute of Physiology, Czech Academy of Sciences