Lipolysis Induced by Alloxan
in Rat Adipocytes is Not Inhibited by Insulin
K. KANDULSKA, T. SZKUDELSKI, L.
NOGOWSKI
Department of Animal Physiology and
Biochemistry, University of Agriculture, Poznań, Poland
Received June 12, 1998
Accepted January 7, 1999
Summary
Isolated rat adipocytes were incubated with adrenaline,
adrenaline plus insulin, alloxan or alloxan plus insulin.
Glycerol release was taken as a measure of lipolysis. It was
observed that alloxan in the concentration of 3, 10 and
20 mmol/l intensifies lipolysis in adipocytes in the absence of
adrenaline. Insulin (10-6 mol/l) treatment of cells did not
inhibit lipolysis caused by this compound, but significantly
restricted lipolysis induced by adrenaline (10-6 mol/l). It was
also shown that alloxan in the concentration of 3 and 10 mmol/l
intensified lipolysis stimulated by adrenaline (10-6 mol/l).
Addition of 20 mmol/l of alloxan strongly inhibited glycerol
release in the presence of adrenaline. The results presented
here clearly indicate that the action of alloxan concerns cells
of the white adipose tissue.
Key words
Adipocytes · Alloxan · Lipolysis · Adrenaline · Insulin · Rat
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