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Cross-Talk Between Nitric Oxide and HSP70 in the Antihypotensive Effect of Adaptation to Heat
I. Yu. MALYSHEV1, L.A. BAYDA1, A.I. TRIFONOV2, N.P.
LARIONOV2, L.D. KUBRINA3, V.D. MIKOYAN3, A.F. VANIN3,
E.B. MANUKHINA1
1Institute of General Pathology and Pathophysiology, Moscow, 2Vladimir University, Vladimir and 3Institute
of Chemical Physics, Moscow, Russia
Received July 30, 1999
Accepted September 21, 1999
Summary
In this work, we evaluated the effect of adaptation to heat on the fall of blood pressure
(BP) induced by heat shock (HS) and the interrelation between nitric oxide (NO) and heat
shock protein, HSP70. Experiments were carried out on Wistar rats. It was shown that HS
resulted in a generalized and transient increase in NO production (the electron
paramagnetic resonance method) and a fall of BP from 113± 3 to 88± 1 mm Hg (?<0.05).
Adaptation to heat itself did not affect BP, but completely prevented the NO
overproduction and hypotension induced by HS. The adaptation simultaneously increased the
brain NO-synthase content and induced HSP70 synthesis (the Western blot analysis) in
various organs. Both the antihypotensive effects of adaptation and HSP70 accumulation were
completely prevented by L-NNA, an inhibitor of NO synthesis, or quercetin, an inhibitor of
HSP70 synthesis. The data suggest that adaptation to heat stimulates NO synthesis and NO
activates synthesis of HSP70. HSP70, which hampers NO overproduction, thus restricts the
BP fall induced by heat shock.
Key words
Nitric oxide · Heat shock protein · Heat shock · Adaptation · Hypotension
Reprint requests
Prof. Igor Yu. Malyshev, Institute of General Pathology and Pathophysiology, Baltijskaya
8, Moscow 125315, Russia.
fax: +7(095)151-04-21, e-mail: nii@pathophys.msk.ru
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