Short-Term Changes of NADPH Diaphorase-Exhibiting
Neuronal Pools in the Spinal Cord of Rabbit after Repeated Sublethal Ischemia
J. Maršala, P. Jalč
Institute of Neurobiology, Slovak Academy of Sciences, Košice, Slovak Republic
Received July 30, 1999
Accepted September 21, 1999
Summary
The aim of this study was the histochemical characterization of NADPH diaphorase-positive
neuronal pools in the rabbit lumbosacral segments using a model of single, repeated and
multiple sublethal spinal cord ischemia. Following a single 8-min sublethal spinal cord
ischemia and 1-hour reperfusion, the staining of NADPH diaphorase-exhibiting neurons in
the dorsal horn, pericentral region, dorsal gray commissure and sacral parasympathetic
nucleus was comparable with the control sections. In contrast to the foregoing sublethal
ischemia, a regionally different somatic NADPH diaphorase (NADPHd) staining was found
after multiple sublethal spinal cord ischemia. Whereas an almost complete loss of the
staining of large NADPHd-exhibiting somata in the pericentral region was detected, the
staining of the NADPHd-exhibiting neuronal pools in the deep dorsal horn and sacral
parasympathetic nucleus was fully preserved. Concomitantly, a prominent reduction of small NADPH diaphorase -positive neurons was noted in the superficial dorsal horn layers of
lower lumbar and sacral segments.
Key words
NADPH diaphorase · Rabbit · Spinal cord · Sublethal ischemia
Reprint requests
Prof. MUDr. J. Maršala, DrSc., Institute of Neurobiology, Slovak Academy of Sciences, Šoltésovej 4, 040 01 Košice, Slovak Republic. e-mail: marsala@saske.sk
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