Effect of
Leptin and Insulin on Chick Embryonic Muscle
Cells and Hepatocytes
D.
LAMOŠOVÁ1, M. ZEMAN1,2
1Institute
of Animal Biochemistry and Genetics, Slovak
Academy of Sciences, Ivanka pri Dunaji, and 2Department
of Animal Physiology and Ethology, Comenius
University, Bratislava, Slovak Republic
Received May 9, 2000
Accepted August 14, 2000
Summary
In the present study we used the primary
cultures of chick embryonic muscle and liver
cells as a model for potential mutual combination
effects of leptin and insulin, respectively. The
influence of both hormones on the proliferation
and protein synthesis was dose-dependent and
related to the age of embryos from which the
cells were isolated. Leptin (10 and 100 ng/well)
increased the proliferation (estimated by DNA
content and incorporation of labeled thymidine
into DNA) and protein synthesis (determined by
incorporation of labeled leucine into proteins)
of muscle cells. The effect of leptin and insulin
in muscle cells was similar. In younger embryo
(11-day-old) the lower dose of leptin was more
effective than the higher one compared to the
insulin effect. Mutual effects of leptin and
insulin were neither additive nor synergistic and
were equivalent to the effects of individual
hormones. In hepatocytes the influence of leptin
was dependent on the age at which the cells were
isolated (11- and 19-day-old embryos). The
presence of insulin neither potentiated nor
inhibited the effect of leptin.
Key
words
Chick embryo · Hepatocytes · Isulin ·
Leptin · Muscle cells
Reprint requests
Ing. Dalma Lamošová, CSc., Institute
of Animal Biochemistry and Genetics, Slovak
Academy of Sciences, Moyzesova 61, 900 28 Ivanka
pri Dunaji. Slovak Republic. Fax: +421-7-45943
932, E-mail: ubzlamo@nic.savba.sk
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