Changes of
Gastric Lipase Activity after Ethanol and
Indomethacin Administration: Influence of
Pretreatment with Allopurinol, Pentoxifylline and
L-DOPA
A. SEDLÁKOVÁ, A.
KOHÚT, M. ŠARIŠSKÝ
Department
of Pharmacology, Faculty of Medicine, P.J.
Šafárik University, Košice, Slovak Republic
Received
February 2, 2000
Accepted October 12, 2000
Summary
Gastric
lipase (GL) plays an important role in
emulsification and digestion of food fat. Lipids
are components of the hydrophobic mucus and
mucosa barrier. Damage of the gastric mucosa may
therefore be related to changes in the lipid
content and GL activity. In the present paper, we
studied the effect of administration of a single
dose of 96 % ethanol (E) and indomethacin 20
mg.kg-1 (IND) on the activity of GL and on the
concentrations of nonesterified fatty acids
(NEFA) and triacylglycerols (TG) in the gastric
mucosa of rats. Furthermore, we studied how these
changes are affected by allopurinol (ALO),
pentoxifylline (PX) and L-DOPA pretreatment 30
min before administration of E or IND. The effect
of sialoadenectomy (SA) on these parameters was
also evaluated. We found: 1) significant
(p<0.01) inhibition of GL activity after
administration of E and IND and also ALO, as well
as after pretreatment with ALO before E and PX
before IND. L-DOPA administered alone stimulated
GL activity, but its administration before IND
significantly (p<0.01) inhibited this
enzymatic activity. GL activity was decreased to
the threshold values in SA rats and after
administration of E to SA animals. 2) NEFA
concentrations were decreased after E and
increased significantly (p<0.01) after IND
administration. A marked significant (p<0.01)
decrease in NEFA was found after PX and L-DOPA
administration. The administration of ALO also
lowered the concentration of NEFA. Pretreatment
by drugs before E and IND resulted in a
significant increase of NEFA in comparison with
the drugs given alone (p<0.05 for ALO + E;
p<0.01 for PX + IND). 3) TG were also
decreased in all experimental groups in
comparison with the control group, i.e. after E
and IND, after ALO and SA and also after
pretreatment by ALO before E. The concentration
of TG decreased after PX, significantly
(p<0.05) after L-DOPA and after pretreatment
by PX before IND. Pretreatment by ALO before E
and L-DOPA before IND resulted in the increase of
TG in comparison with drugs alone. Thus, these
results suggest certain protective effect of
pretreatment with ALO, PX and L-DOPA against the
E- and IND-induced decrease in NEFA and TG during
injury of the gastric mucosa. On the other hand,
inhibition of GL activity was also apparent after
administration of these drugs before E and IND,
which suggest presence of a persisting impairment
of lipid digestion in the stomach.
Key
words
Gastric
lipase · Lipids · Ethanol · Indomethacin ·
Allopurinol · Pentoxifylline · L-DOPA
Reprint
requests
Anton Kohút, Department of Pharmacology, Faculty
of Medicine, P.J. Šafárik University, Tr. SNP
1, 040 66 Košice, Slovak Republic, akohut@central.medicin.upjs.sk
|