Antioxidant
Vitamin Levels and Glutathione Peroxidase
Activity During Ischemia/Reperfusion in
Myocardial Infarction
V. MUŽÁKOVÁ, R.
KANĎÁR, P. VOJTÍŠEK1, J. SKALICKÝ2,
R. VAŇKOVÁ3, A. ČEGAN, Z.
ČERVINKOVÁ4
Department
of Biological and Biochemical Sciences, Faculty
of Chemical Technology, University of Pardubice, 1Department
of Cardiology, Hospital of Pardubice, 2Department
of Clinical Biochemistry, Hospital of Pardubice, 3Institute
of Experimental Botany, Academy of Sciences of
the Czech Republic, Prague and 4Department
of Physiology, Faculty of Medicine, Charles
University, Hradec Králové, Czech Republic
Received September 11,
2000
Accepted January 19, 2001
Summary
The
consequences of increased oxidative stress,
measured as the level of malondialdehyde (MDA)
during ischemia/reperfusion, were studied in 48
patients in the acute phase of myocardial
infarction (AMI) and a control group (21 blood
donors). The serum levels of a-tocopherol and
b-carotene were followed. Immediately after the
treatment onset the level of a-tocopherol started
to decrease, reaching a plateau after 24 h. The
consumption of b-carotene was delayed by 90 min.
Steady decline was detected during the whole time
interval studied (48 h). Glutathione peroxidase
(GPx) activity, as a representative of
antioxidant enzymes, was estimated in whole
blood. The influx of oxygenated blood was
accompanied by a stimulation of GPx activity,
which reached its maximum at the time of
completed reperfusion. When comparing the AMI
patients with the control group, the levels of
MDA were found significantly increased, which
indicates that oxidative stress is already
increased during ischemia. Lower antioxidant
levels found in the patients might either already
be the result of vitamin consumption during
ischemia or be a manifestation of their
susceptibility to AMI. Monitored consumption of
a-tocopherol and b-carotene during reperfusion
indicated that in the case of patients, whose
level of antioxidant vitamins is below the
threshold limit, a further substantial decrease
of antioxidant vitamins during reperfusion could
enhance the oxidative damage of the myocardium.
Key
words
Myocardial
infarction · Oxidative stress · Malondialdehyde
· Glutathione peroxidase · Alpha tocopherol ·
Beta carotene
Reprint
requests
V.
Mužáková, M.D., Department of Biological and
Biochemical Sciences, Faculty of Chemical
Technology, University of Pardubice, Štrossova
239, 530 03 Pardubice, Czech Republic. e-mail: vladimira.muzakova@upce.cz
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