| Improvement of the
Accuracy by the Measurement of the Electrical
Cell Membrane Parameters
V.
ROHLÍČEK, F. RECH
Institute of Physiology, Czech Academy of
Sciences, Prague, Czech Republic
Received April 19, 201
Accepted June 29, 2001
Summary
The electrical parameters of the cell
membrane are mostly estimated employing ac
methods. The measurement is based on the analysis
of the current(s) flowing through an access
resistance and the membrane. A current/potential
transducer is used at the input of the device.
The parameters of this transducer, especially its
feedback capacity, degrades the accuracy of the
measurement and hence diminishes the suppression
of mutual influences of the individual
parameters. The paper suggests a possible
software correction and is supplemented by
remarks for practical application.
Key
words
Patch
clamp technique · Electrical parameters of the
cell membrane · Membrane capacity · Membrane
conductance · Access resistance · Dual
frequency method · Exocytosis
Reprint
requests
V. Rohlíček, Institute of Physiology, Academy
of Sciences of the Czech Republic, Vídeňská
1083, 142 20 Prague 4, Czech Republic. E-mail : Rohlicek@biomed.cas.cz
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