| Streptozotocin
Induces Lipolysis in Rat Adipocytes in Vitro
T.
SZKUDELSKI, K. SZKUDELSKA
Department
of Animal Physiology and Biochemistry, University
of Agriculture, Poznań, Poland
Received May 14, 2001
Accepted September 11, 2001
Summary
Streptozotocin (STZ) is used to induce
experimental diabetes in animals and is also
applied for the treatment of patients with
insulinoma. The aim of the present work was to
investigate the direct effect of STZ on lipolysis
in isolated rat adipocytes. After the isolation,
the cells were incubated in a Krebs-Ringer buffer
of pH 7.4, at the temperature 37 °C for 90 min
with different concentrations of STZ: 0.5, 1 or 2
mmol/l. STZ caused a significant rise in basal
values (99 %, 199 %, and 377 %, respectively) and
epinephrine-stimulated (1 µmol/l) lipolysis (15
%, 24 % and 46 %, respectively). Augmentation of
basal lipolysis by STZ was neither restricted by
insulin (1 nmol/l) nor by H-89 (an inhibitor of
protein kinase A, 50 µmol/l). These results
indicate the stimulatory influence of STZ on the
action of hormone-sensitive lipase in isolated
cells of white adipose tissue. The obtained
outcomes suggest that in studies employing STZ,
it is necessary to consider its direct effect
upon lipolysis in adipocytes.
.
Key
words
Adipocytes
· Streptozotocin · Lipolysis
Reprint
requests
Tomasz Szkudelski, Department of Animal
Physiology and Biochemistry, University of
Agriculture, 60-637 Wołyńska 35, Poznań,
Poland, fax: +48 61 8487196, e-mail: tszkudel@jay.au.poznan.pl
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